Let be a weighted fractional Brownian motion with indices and satisfying . In this paper, motivated by the asymptotic property for all , we consider the generalized quadratic covariation defined by provided the limit exists uniformly in probability. We construct a Banach space of measurable functions such that the generalized quadratic covariation exists in and the generalized Bouleau–Yor identity holds for all , where is the weighted local time of and is the Beta function.