In this work, the core–shell bovine hemoglobin (BHb)-imprinted superparamagnetic nanoparticles (Fe₃O₄@BHb-MIPs) were synthesized by combining for the first time a surface imprinting technique and a two-step immobilized template strategy. Initially, amino-functionalized Fe₃O₄ nanoparticles (Fe₃O₄@NH₂) were synthesized directly through a facile one-pot hydrothermal method. Next, BHb was immobilized on the surface of Fe₃O₄@NH₂ through non-covalent interactions. Then, siloxane co-polymerization on the Fe₃O₄@NH₂–protein complex surface resulted in a polymeric network molded around BHb which then became further immobilized. Finally, a thin polymer layer with specific recognition cavities for BHb was formed on the surface of Fe₃O₄@NH₂ after the removal of the template protein. The morphology and structure property of the prepared magnetic nanoparticles were characterized by transmission electronic microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometer (XRD), and vibrating sample magnetometer (VSM). To obtain the best selectivity and binding performance, the polymerization and adsorption conditions were investigated in detail. Under the optimized conditions, the Fe₃O₄@BHb-MIPs exhibited fast adsorption kinetics, large binding capacity, significant selectivity, and favorable reproducibility. The resultant Fe₃O₄@BHb-MIPs could not only specifically extract BHb from a mixed standard protein mixture, but also selectively enriched BHb from a real bovine blood sample. In addition, the synthetic process was quite simple and the stability and regeneration of the Fe₃O₄@BHb-MIPs were also satisfactory.