Readily available environmental covariates in current digital soil mapping usually do not indicate the spatial differences between deep soil attributes. This, to a large extent, leads to a decrease in the accuracy of 3D soil mapping with depth, which seriously affects the quality of soil information generated. This study tested the hypothesis that spatialized laboratory soil spectral information can be used as environmental covariates to improve the accuracy of 3D soil attribute mapping and proposed a new type of environmental covariable. In the first step, with soil-forming environmental covariates and independent soil profiles, laboratory vis-NIR spectral data of soil samples resampled into six bands in Anhui province, China, were spatially interpolated to generate spatial distributions of soil spectral measurements at multiple depths. In the second step, we constructed three sets of covariates using the laboratory soil spectral distribution maps at multiple depths: conventional soil-forming variables (C), conventional soil-forming variables plus satellite remote sensing wavebands (C+SRS) and conventional soil-forming variables plus spatialized laboratory soil spectral information (C+LSS). In the third step, we used the three sets of environmental covariates to develop random forest models for predicting soil attributes (pH; CEC, cation exchange capacity; Silt; SOC, soil organic carbon; TP, total phosphorus) at multiple depths. We compared the 3D soil mapping accuracies between these three sets of covariates based on another dataset of 132 soil profiles (collected in the 1980s). The results show that the use of spatialized laboratory soil spectral information as additional environmental covariates has a 50% improvement in prediction accuracy compared with that of only conventional covariates, and a 30% improvement in prediction accuracy compared with that of the satellite remote sensing wavebands as additional covariates. This indicates that spatialized laboratory soil spectral information can improve the accuracy of 3D digital soil mapping.