Objective : The aim of our study is to investigate the cytotoxic, antioxidant, and antimicrobial effects of newly synthesized boron compounds in U87MG glioblastoma cell treatment.Methods : We synthesized boron glycine monoester (BGM) and boron glycine diester (BGD) structures containing boron atoms and determined their cytotoxic activities on glioblastoma by the MTT method. The inhibitory concentration 50 (IC50) value was calculated with GraphPad Prism 5.0 program. The IC50 values were administered 48 hours on U87MG glioblastoma cell. Catalase (CAT), acid phosphatase (ACP) and alkaline phosphatase (ALP) enzyme activity, malondialdehyde (MDA), total glutathione (GSH), and total protein levels were detected using spectrophotometric methods. We determined the antimicrobial activities of BGM and BGD with the disc diffusion method.Results : After 48 hours of BGM and BGD application to U87MG glioblastoma cells, we found the IC50 value as 6.6 mM and 26 mM, respectively. CAT and ACP enzyme activities were decreased in BGM and BGD groups. MDA which is a metabolite of lipid peroxidation was increased in both boron compounds groups. GSH level was reduced especially in BGD group. BGM and BGD have been found to be antimicrobial effects.Conclusion : Boron compounds, especially the BGM, can provide a new therapeutic approach for the treatment of glioblastoma with their anticancer, antioxidant, and antimicrobial effects.

This study aims to determine the toxic effects of heavy metal pollution on carp (Cyprinus carpio) in Sapanca Lake by biochemical and histological analyses. For this reason, fish and water samples were taken from the lake in 2015. Heavy metal (Cu, Fe, Zn, Pb, Cd) analyzes in the water column and tissues (muscle, liver, gill) were determined by ICP-OES. CAT, GSH and MDA levels, which are oxidative stress bioindicators in tissues, were measured by spectrophotometric methods. Histopathological findings in tissues were determined by Hematoxylin-Eosin staining. As a result, heavy metal concentrations in water were determined as Fe > Zn > Pb > Cu > Cd. The accumulation of Cu, Fe and Cd in the tissues of the fish were liver > gill > muscle, and the accumulation of Zn was gill > liver > muscle. CAT activity, MDA and GSH level of the tissues changed with the water temperature. General signs of destruction were observed in the gill tissues of the fish. Necrotic conditions in hepatocytes were observed. In conclusion, the presence of biochemical and histopathological findings in tissues suggests that the lake is not only affected by heavy metals but also by other pollutants.