Leaf area index (LAI) and leaf dry matter (LDM) are important indices of crop growth. Real-time, nondestructive monitoring of crop growth is instructive for the diagnosis of crop growth and prediction of grain yield. Unmanned aerial vehicle (UAV)-based remote sensing is widely used in precision agriculture due to its unique advantages in flexibility and resolution. This study was carried out on wheat trials treated with different nitrogen levels and seeding densities in three regions of Jiangsu Province in 2018–2019. Canopy spectral images were collected by the UAV equipped with a multi-spectral camera during key wheat growth stages. To verify the results of the UAV images, the LAI, LDM, and yield data were obtained by destructive sampling. We extracted the wheat canopy reflectance and selected the best vegetation index for monitoring growth and predicting yield. Simple linear regression (LR), multiple linear regression (MLR), stepwise multiple linear regression (SMLR), partial least squares regression (PLSR), artificial neural network (ANN), and random forest (RF) modeling methods were used to construct a model for wheat yield estimation. The results show that the multi-spectral camera mounted on the multi-rotor UAV has a broad application prospect in crop growth index monitoring and yield estimation. The vegetation index combined with the red edge band and the near-infrared band was significantly correlated with LAI and LDM. Machine learning methods (i.e., PLSR, ANN, and RF) performed better for predicting wheat yield. The RF model constructed by normalized difference vegetation index (NDVI) at the jointing stage, heading stage, flowering stage, and filling stage was the optimal wheat yield estimation model in this study, with an R² of 0.78 and relative root mean square error (RRMSE) of 0.1030. The results provide a theoretical basis for monitoring crop growth with a multi-rotor UAV platform and explore a technical method for improving the precision of yield estimation.

Leaf area index (LAI) is a fundamental indicator of plant growth status in agronomic and environmental studies. Due to rapid advances in unmanned aerial vehicle (UAV) and sensor technologies, UAV-based remote sensing is emerging as a promising solution for monitoring crop LAI with great flexibility and applicability. This study aimed to determine the feasibility of combining color and texture information derived from UAV-based digital images for estimating LAI of rice (Oryza sativa L.). Rice field trials were conducted at two sites using different nitrogen application rates, varieties, and transplanting methods during 2016 to 2017. Digital images were collected using a consumer-grade UAV after sampling at key growth stages of tillering, stem elongation, panicle initiation and booting. Vegetation color indices (CIs) and grey level co-occurrence matrix-based textures were extracted from mosaicked UAV ortho-images for each plot. As a solution of using indices composed by two different textures, normalized difference texture indices (NDTIs) were calculated by two randomly selected textures. The relationships between rice LAIs and each calculated index were then compared using simple linear regression. Multivariate regression models with different input sets were further used to test the potential of combining CIs with various textures for rice LAI estimation. The results revealed that the visible atmospherically resistant index (VARI) based on three visible bands and the NDTI based on the mean textures derived from the red and green bands were the best for LAI retrieval in the CI and NDTI groups, respectively. Independent accuracy assessment showed that random forest (RF) exhibited the best predictive performance when combining CI and texture inputs (R² = 0.84, RMSE = 0.87, MAE = 0.69). This study introduces a promising solution of combining color indices and textures from UAV-based digital imagery for rice LAI estimation. Future studies are needed on finding the best operation mode, suitable ground resolution, and optimal predictive methods for practical applications.

The Soil Plant Analysis Development (SPAD) chlorophyll meter is one of the most commonly used diagnostic tools to measure crop nitrogen status. However, the measurement method of the meter could significantly affect the accuracy of the final estimation. Thus, this research was undertaken to develop a new methodology to optimize SPAD meter measurements in rice (Oryza sativa L.). A flatbed color scanner was used to map the dynamic chlorophyll distribution and irregular leaf shapes. Calculus algorithm was adopted to estimate the potential positions for SPAD meter measurement along the leaf blade. Data generated by the flatbed color scanner and SPAD meter were analysed simultaneously. The results suggested that a position 2/3 of the distance from the leaf base to the apex (2/3 position) could represent the chlorophyll content of the entire leaf blade, as indicated by the relatively low variance of measurements at that positon. SPAD values based on di-positional leaves and the extracted chlorophyll a and b contents were compared. This comparison showed that the 2/3 position on the lower leaves tended to be more sensitive to changes in chlorophyll content. Finally, the 2/3 position and average SPAD values of the fourth fully expanded leaf from the top were compared with leaf nitrogen concentration. The results showed the 2/3 position on that leaf was most suitable for predicting the nitrogen status of rice. Based on these results, we recommend making SPAD measurements at the 2/3 position on the fourth fully expanded leaf from the top. The coupling of dynamic chlorophyll distribution and irregular leaf shapes information can provide a promising approach for the calibration of SPAD meter measurement, which can further benefit the in situ nitrogen management by providing reliable estimation of crops nitrogen nutrition status.

Predicting the grain yield during early to mid-growth stages is important for initial diagnosis of rice and quantitative regulation of topdressing. In this study, we conducted four experiments using different nitrogen (N) application rates (0–400 kg N∙ha⁻¹) in three Japonica rice cultivars (Wuyunjing24, Ningjing4, and Lianjing7) grown in Jiangsu province, Eastern China, from 2015–2016. Spectral reflectance data were collected multiple times during early to mid-growth stages using an active mounted sensor (RapidScan CS-45, Holland Scientific Inc., Lincoln, NE, USA). Data were then used to calculate optimal vegetation indexes (normalized difference red edge, NDRE; normalized difference vegetation index, NDVI; ratio vegetation index, RVI; red-edge ratio vegetation index, RERVI), which were used to develop a dynamic change model and in-season grain yield prediction model. The NDRE index was more stable than other indexes (NDVI, RVI, RERVI), showing less standard deviation at the same N fertilizer rate. The R² of the relationships between leaf area index (LAI), plant nitrogen accumulation (PNA), and NDRE also increased compared to other indexes. These findings suggest that NDRE is suitable for analysis of paddy rice N nutrition. According to real-time series changes in NDRE, the resulting dynamic model followed a sigmoid curve, with a coefficient of determination (R²) >0.9 and relative root-mean-square error 0.7. For every 0.1 increase in SV, grain yield increased by 3608.1 kg·ha⁻¹. Overall, our new dynamic model effectively predicted grain yield at stem elongation and booting stages, providing real-time crop N nutrition data for management of N fertilizer topdressing in rice production.

The accurate estimation of aboveground biomass (AGB) and leaf area index (LAI) is critical to characterize crop growth status and predict grain yield. Unmanned aerial vehicle (UAV) -based remote sensing has attracted significant interest due to its high flexibility and easiness of operation. The mixed effect model introduced in this study can capture secondary factors that cannot be captured by standard empirical relationships. The objective of this study was to explore the potential benefit of using a linear mixed-effect (LME) model and multispectral images from a fixed-wing UAV to estimate both AGB and LAI of rice. Field experiments were conducted over two consecutive years (2017–2018), that involved different N rates, planting patterns and rice cultivars. Images were collected by a compact multispectral camera mounted on a fixed-wing UAV during key rice growth stages. LME, simple regression (SR), artificial neural networks (ANN) and random forests (RF) models were developed relating growth parameters (AGB and LAI) to spectral information. Cultivar (C), growth stage (S) and planting pattern (P) were selected as candidates of random effects for the LME models due to their significant effects on rice growth. Compared to other regression models (SR, ANN and RF), the LME model improved the AGB estimation accuracy for all stage groups to varying degrees: the R² increased by 0.14–0.35 and the RMSE decreased by 0.88–1.80 t ha⁻¹ for the whole season, the R² increased by 0.07–0.15 and the RMSE decreased by 0.31–0.61 t ha⁻¹ for pre-heading stages and the R² increased by 0.21–0.53 and the RMSE decreased by 0.72–1.52 t ha⁻¹ for post-heading stages. Further analysis suggested that the LME model also successfully predicted within the groups when the number of groups was suitable. More importantly, depending on the availability of C, S, P or combinations thereof, mixed effects could lead to an outperformance of baseline retrieval methods (SR, ANN or RF) due to the inclusion of secondary effects. Satisfactory results were also obtained for the LAI estimation while the superiority of the LME model was not as significant as that for AGB estimation. This study demonstrates that the LME model could accurately estimate rice AGB and LAI and fixed-wing UAVs are promising for the monitoring of the crop growth status over large-scale farmland.

Rapid and effective acquisition of crop growth information is a crucial step of precision agriculture for making in-season management decisions. Active canopy sensor GreenSeeker (Trimble Navigation Limited, Sunnyvale, CA, USA) is a portable device commonly used for non-destructively obtaining crop growth information. This study intended to expand the applicability of GreenSeeker in monitoring growth status and predicting grain yield of winter wheat (Triticum aestivum L.). Four field experiments with multiple wheat cultivars and N treatments were conducted during 2013–2015 for obtaining canopy normalized difference vegetation index (NDVI) and ratio vegetation index (RVI) synchronized with four agronomic parameters: leaf area index (LAI), leaf dry matter (LDM), leaf nitrogen concentration (LNC), and leaf nitrogen accumulation (LNA). Duration models based on NDVI and RVI were developed to monitor these parameters, which indicated that NDVI and RVI explained 80%, 68–70%, 10–12%, and 67–73% of the variability in LAI, LDM, LNC and LNA, respectively. According to the validation results, the relative root mean square error (RRMSE) were all <0.24 and the relative error (RE) were all <23%. Considering the variation among different wheat cultivars, the newly normalized vegetation indices rNDVI (NDVI vs. the NDVI for the highest N rate) and rRVI (RVI vs. the RVI for the highest N rate) were calculated to predict the relative grain yield (RY, the yield vs. the yield for the highest N rate). rNDVI and rRVI explained 77–85% of the variability in RY, the RRMSEs were both <0.13 and the REs were both <6.3%. The result demonstrates the feasibility of monitoring growth parameters and predicting grain yield of winter wheat with portable GreenSeeker sensor.