Effects of the antiosteoblastogenesis factor Semaphorin 4D (Sema4D), expressed by thrombin-activated platelets (TPs), on osteoblastogenesis, as well as osteoclastogenesis, were investigated in vitro. Intact platelets released both Sema4D and IGF-1. However, in response to stimulation with thrombin, platelets upregulated the release of Sema4D, but not IGF-1. Anti-Sema4D-neutralizing monoclonal antibody (mAb) upregulated TP-mediated osteoblastogenesis in MC3T3-E1 osteoblast precursors. MC3T3-E1 cells exposed to TPs induced phosphorylation of Akt and ERK further upregulated by the addition of anti-sema4D-mAb, suggesting the suppressive effects of TP-expressing Sema4D on osteoblastogenesis. On the other hand, TPs promoted RANKL-mediated osteoclastogenesis in the primary culture of bone-marrow-derived mononuclear cells (BMMCs). Among the known three receptors of Sema4D, including Plexin B1, Plexin B2 and CD72, little Plexin B2 was detected, and no Plexin B1 was detected, but a high level of CD72 mRNA was detected in RANKL-stimulated BMMCs by qPCR. Both anti-Sema4D-mAb and anti-CD72-mAb suppressed RANKL-induced osteoclast formation and bone resorptive activity, suggesting that Sema4D released by TPs promotes osteoclastogenesis via ligation to a CD72 receptor. This study demonstrated that Sema4D released by TPs suppresses osteogenic activity and promotes osteoclastogenesis, suggesting the novel property of platelets in bone-remodeling processes.

In Indonesia, stunting continues to be a problem for public health, even in Pasuruan City. In Pasuruan City, 1,516 kids still run the risk of developing stunting. The Pasuruan City Government works to keep the rate of stunting under 14%. To reduce the likelihood that children with stunting will be born, pregnant mothers are the main focus of prevention efforts. Inflammatory diseases in the mouth can set off pregnancy complications that have the potential to result in preterm birth, low birth weight babies (LBW), and eventually become the cause of stunting in children. This community service is carried out in the form of training and counseling on periodontal tissue and oral health for pregnant women and health cadres at the Pasuruan City Health Center to increase knowledge of stunting prevention in Pasuruan City. Community service was carried out at four health centers in Pasuruan City, namely the Kandangsapi, Bugul Kidul, Kebonsari, and Trajeng Health Centers. The target partners in community service are health cadres and pregnant women in the work area of ​​each health center. The form of service carried out is counseling and training. Education initiatives were conducted on the subject of periodontal tissue health, its relationship to pregnancy, the prevalence of LBW, and the possibility of stunting effects on the periodontal tissue health of pregnant women. The cognitive and psychomotor health of cadres and pregnant women can be improved by community service in the form of counseling and training.  

Periodontitis remains a significant global health burden, and its accurate diagnosis is critical for effective management. The 2018 classification system for periodontitis introduces staging, grading, and extent parameters to enhance diagnostic consistency, but its application among trainees remains underexplored. This study evaluates the consistency of staging, grading, and extent diagnosis of periodontitis among Indonesian periodontics specialist students using the 2018 classification system, highlighting challenges and variability in interpretation. A total of 41 students across different training levels participated in the study, classifying 5 clinical cases based on comprehensive diagnostic materials, including radiographs, periodontal charts, and medical histories. Diagnostic concordance with gold-standard diagnoses was assessed and using Fleiss Kappa for inter-reliability analysis. Agreement rates were 56.10% for staging (Fleiss’ Kappa = 0.170, 95% CI: 0.145–0.196), 76.10% for grading (Kappa = 0.210, 95% CI: 0.185–0.235), and 74.15%. for extent (Kappa = 0.168, 95% CI: 0.144–0.192). Overestimation of severity occurred in 35.12% of staging decisions, while 20.93% of cases were underestimated in grading. Diagnostic variability was greatest in borderline cases. Indonesian periodontics specialist students demonstrated fair consistency in grading but poor agreement in staging and extent compared with expert consensus. These findings highlight the need for enhanced calibration exercises and curriculum reform to improve diagnostic reliability. Multi-institutional studies with larger cohorts are recommended to further validate these findings.