Demand for new food sources and production methods is increasing due to overall population growth, as well as the aim towards more sustainable use of natural resources and circular economy. Edible insects already used in many parts of the world have recently attracted interest as a new protein source in Europe, and novel food acceptance procedures are ongoing in the European Union for several insect species. In this paper, the effects of substate on the nutritional value, especially the fatty acid composition, of edible insects were reviewed and correlation calculations performed. The nutritional value of edible insects is an important health aspect, in particular, a high content of good-quality protein and unsaturated fatty acids with essential fatty acids, and an optimal fatty acid n6/n3 ratio. On the basis of our findings, the nutrient content of insects can be modified by using a feed substrate carefully designed for each individual insect species. In addition, our correlation calculations demonstrated that the contents of linoleic and alpha linolenic acids in insects reflected the contents of these acids in the substrate. In conclusion, optimizing the composition and structure of the substrate and rearing conditions and duration for each insect species might also aid standardization of the nutritional composition of edible insects.

High-pressure processing (HPP) is a promising method for preserving food, for example by inactivating pathogens and spoilage microbes. However, there is still a lack of knowledge about the optimal processing parameters for different food products. The aim of this study was to assess the effectiveness of different pressures to inactivate Listeria monocytogenes in cold-smoked and warm-smoked rainbow trout (Oncorhynchus mykiss) ready-to-eat (RTE) fish products. L. monocytogenes ATCC 7644 was inoculated into sliced cold-smoked rainbow trout fillets and whole warm-smoked rainbow trout fillets. The fish samples were pressure-treated at three different pressures, either at 200, 400, or 600 MPa, at 4 ± 1 °C for 3 min in each pressure. Bacterial enumeration of the samples and control samples were analysed 1, 14, and 28 days after the HPP treatment by using serial dilution and the spread plate technique. Based on the study results, the most effective pressure was 600 MPa and the number of L. monocytogenes colonies, both in cold-smoked and in warm-smoked fish samples, was within the official limit of the Regulation (EC) No. 2073/2005 (100 cfu/g) even after 28 days of storage.

The aim of this study was to evaluate the antimicrobial activity of wood distillates obtained from Scots pine (Pinus sylvestris) sawdust in order to explore new alternatives for the utilization of wood industry by-products. The distillates were produced by slow pyrolysis thermal conversion in three process phases with increasing temperatures, namely drying, torrefaction and pyrolysis, and three cooling units with different temperatures to condensate the distillates. This yielded nine different liquid fractions. The food-related pathogens, Salmonella, Listeria monocytogenes and Candida albicans, were evaluated for their susceptibility to the distillate fractions using an agar diffusion test. The antimicrobial activity was estimated by measuring the formed inhibition zones after the incubation period. In addition, the minimum inhibitory concentration (MIC) and microbicidic concentration were assayed for a selected fraction (T2) from the torrefaction phase with Bio-screen C. The results indicated that the distillates from the torrefaction and pyrolysis phases had antimicrobial activity against the tested microbes. The MIC value of the T2 fraction for all tested microbes was 0.83% (v/v). Furthermore, the T2 fraction was microbicidic for Salmonella and Listeria strains in 0.83% (v/v) solution and Candida strain in 1.67% (v/v) solution. In conclusion, Scots pine wood distillates obtained from slow pyrolysis have the potential to be developed as antimicrobial agents against pathogenic microbes. Next, research is needed to investigate the chemical composition of the distillates and to assess their safe use.