The occurrence of Aeromonas spp. in the Porsuk River, public drinking water and tap water in the City of Eskisehir (Turkey) was monitored. Fresh water samples were collected from several sampling sites during a period of one year. Total 102 typical colonies of Aeromonas spp. were submitted to biochemical tests for species differentiation and of 60 isolates were confirmed by biochemical tests. Further identifications of isolates were carried out first with the VITEK system (BioMe˜rieux) and then selected isolates from different phenotypes (VITEK types) were identified using the DuPont Qualicon RiboPrinter® system. Aeromonas spp. was detected only in the samples from the Porsuk River. According to the results obtained with the VITEK system, our isolates were 13% Aeromonas hydrophila, 37% Aeromonas caviae, 35% Pseudomonas putida, and 15% Pseudomonas acidovorans. In addition Pseudomonas sp., Pseudomonas maltophila, Aeromonas salmonicida, Aeromonas hydrophila, and Aeromonas media species were determined using the RiboPrinter® system. The samples taken from the Porsuk River were found to contain very diverse Aeromonas populations that can pose a risk for the residents of the city. On the other hand, drinking water and tap water of the City are free from Aeromonas pathogens and seem to be reliable water sources for the community.

Enterococcus faecium, a lactic acid bacterium (LAB), was evaluated for its ability to remove copper(II) ions from water. The effects of the pH, contact time, initial concentration of copper(II) ions, and temperature on the biosorption rate and capacity were studied. The initial concentrations of copper(II) ions used to determine the maximum amount of biosorbed copper(II) ions onto lyophilised lactic acid bacterium varied from 25 mg L-1 to 500 mg L-1. Maximum biosorption capacities were attained at pH 5.0 and 6.0. Temperature variation between 20°C and 40°C did not affect the biosorption capacity of the bacterial biomass. The highest copper(II) ion removal capacity was 106.4 mg per g dry biomass. The correlation regression coefficients show that the biosorption process can be well defined by the Freundlich equation. The change in biosorption capacity with time was found to fit a pseudo-second-order equation

New N-(naphthalen-1-yl)propanamide was synthesized and studied for their antimicrobial activity. The final compounds were procured by reacting N-(naphthalen-1-yl)propanamide with some 2-mercapto aryl or dithiocarbamate salt derivatives. Their structures were determined by ¹H-NMR and ¹³C-NMR spectroscopy and LC-MS/MS spectral data. The synthesized compounds were investigated for their antimicrobial activities against 10 bacteria and 10 fungi species. All compounds showed notable activity. Especially, compounds 2-[(5-methyl-1,3,4-thiadiazol-2-yl)thio]-N-(naphthalen-1-yl)propanamide (2a), 2-(benzothiazol-2-ylthio)-N-(naphthalen-1-yl)propenamide (2b), 2-[(1-methyl-1H-imidazol-2-yl)thio]-N-(naphthalen-1-yl)propanamide (2c), and N-(naphthalen-1-yl)-2-[(5-nitro-1H-benzimidazol-2-yl)thio]propanamide (2e) exhibited antifungal activity against at least one fungus at the half potency of ketoconazole. Also, 2-[(1-methyl-1H-imidazol-2-yl)thio]-N-(naphthalen-1-yl)propanamide (2c), N-(naphthalen-1-yl)-2-[(5-nitro-1H-benzimidazol-2-yl)thio]propanamide (2e) and 2-[(4-methyl-4H-1,2,4-triazol-3-yl)thio]-N-(naphthalen-1-yl)propanamide (2f) showed anti-gram-positive bacterial activity at the half potency of chloramphenicol. Only one compound, 2-(benzothiazol-2-ylthio)-N-(naphthalen-1-yl)propanamide (2b) displayed anti-gram-negative bacterial activity against Yersinia enterocolitica (Y53).

In this work, six 2-[(1/4-methylimidazol/triazol/tetrazol- 2/3/5-yl)thio]-N-(4-substituted thiazol-2-yl) acetamide derivatives (2a-f) were synthesized. The antimicrobial activities of these substances were examined against some foodborne Gram positive and Gram negative test bacteria, Candida albicans, Candida glabrata, Candida krusei, and Candida parapsilosis yeasts, and some Aspergillus and Penicillium filamentous fungi species. The substances were shown considerable antimicrobial effect to tested pathogenic microorganisms.

This study reports kinetics and equilibrium of lead sorption onto the biomass of Enterococcus faecium. E. faecium is a lactic acid bacterium and was isolated from meat. Batch experiments were carried out to analyze the effects of the initial lead concentration, initial pH of the medium, agitation time and temperature on the biosorption. The lead sorption was found to increase with the increase in the solution pH, reaching a plateau value beyond pH 5, and the most favorable pH for removal was determined as 5.0. The highest lead uptake capacity of the biomass was obtained at the initial lead concentration of 300 mg L(-1). The Langmuir and Freundlich adsorption models were applied to determine the biosorption isotherm, and the equilibrium data correlated well with the Langmuir model. The pseudo-second-order kinetic model was more suitable to fit the experimental data. The results were promising that the biomass of this lactic acid bacterium can be successfully used as a convenient adsorbent for lead removal from aqueous solutions.