The aim of this study was to evaluate the influence of hidden environmental pollution on some blood parameters of sheep to detect susceptible biomarkers able to reveal slight contamination. Four dairy sheep farms, two with semi-extensive and two with intensive type systems were involved in this study. Two farms in different systems were chosen as properly located in a southern area of Latium (Italy), close to the Sacco River, in which contamination with β-hexachlorocyclohexane (β-HCH) occurred in the past due to industrial waste. A recent study established the presence of low but detectable residual contamination in these areas. The other two farms were outside the contaminated area. Erythrocyte glutathione transferase (e-GST) and oxidative stress parameters were monitored as well as some immune response and metabolic profile parameters throughout the investigated period of four months. The present study showed a relevant and significant increase in e-GST (+63%) in the extensive farming system of the contaminated area, whereas some immune response biomarkers, i.e., white blood cells, neutrophils, lymphocytes, and lysozyme resulted within the physiological range. In all farms, oxidative stress and acute phase response parameters were also within the physiological range. Our results suggest that e-GST is a very effective alarm signal to reveal “hidden” persistent contamination by β-HCH, and reasonably, by many other different dangerous pollutants.

This work reports the results of a study carried out to verify the applicability of the CEN EN 13751 method, based on the photo-stimulated luminescence (PSL) technique, for the identification of irradiated fruit. A total of 15 types of fresh and dried fruit not irradiated and irradiated (0.5 and 1 kGy) were tested. Preliminary tests were performed on samples of fresh kiwis, lemons, mangoes, oranges, papayas, pineapples and tangerines, dried dates, dried prunes and raisins. Successively, an inter-comparative test was organized, whereby four Italian official control laboratories analyzed eight matrices, namely, kiwi, orange, dried fig, raisin, hazelnut, peanut, pistachio and walnut. The results indicated considerable differences in the radiation PSL sensitivities of the different types of fruit as well as among the PSL responses obtained from different batches of the same fruit. The best results were obtained with some types of dried fruit, namely, pistachios, peanuts, dried figs and raisins. Irradiated fresh fruit generally gave non-positive results, mainly intermediate and even very close to the negative threshold. Tests performed on kiwi, mango and orange samples indicated that the parts of the fruit peel close to the stem, which are more wrinkled, provide better results.