This study sought to determine the effect of fermented medicinal herbs (FMH), i.e. cutchery (Kaempferia galanga), turmeric (Curcuma longa) and curcuma (Curcuma xanthorrhiza) in combination with molasses and probiotic drink (Yakult), administered orally on the hematological and physiological profile of striped catfish (Pangasi a nodon hypophthalmus). A complete randomized design (CRD) experiment was used with four levels of treatments, namely P0 (control), P1 (FMH 100 mL/kg), P2 (FMH 200 mL/kg) and P3 (FMH 300 mL/kg) of feed. The fish were kept in a farm in cages at 75 fish/m3 and fed with the experimental diets for 60 days. The results revealed that FMH (P2) dietary administration improved hematological and physiological profile of catfish, i.e total erythrocytes of 2.81 x 106 cells/mm3, hematocrit values of 39.00%, hemoglobin levels of 10.73 g/dL, total leukocytes of 11.41 x 104 cells/mm3, blood glucose 97.33 mg/dL, and total serum protein 4.10 mg/dL compared to controls with 1.89 x 106 cells/mm3, 32.33 %, g/dL, 9.67 x 104 cells/mm3, 67.33 mg/dL, and total serum protein of 3.10 mg/dL, respectively. Moreover, the diet improved special growth rate, feed conversion ratio, feed efficiency and the survival rate of catfish. The hematological and physiological profile of catfish improvement are considered to be due to the content of secondary metabolites of FMH, namely curcuminoids, vitamin C, essential oils, tannins, and flavonoids, which trigger immunostimulation. The presence of curcuminoids provide an antioxidant effect on cell membranes reducing erythrocyte cell membrane damage due to oxidation. Similarly, flavonoids are natural antioxidants, which are credited with the ability of reducing free radicals and anti-free radicals.

One of the plants that have the potential as antibacterial ingredients is Rhizophora apiculata leaf because it contains secondary metabolites, such as tannins, saponins, flavonoids, steroids, and terpenoids. This study aims to find out the inhibition of hot water extract of R. apiculata leaves in inhibiting the growth of Edwardsiella tarda. The research method used is an experimental method which includes sample preparation, extraction, antibacterial activity test using the Kirby-Bauer disc method and applying a Completely Randomized Design (CRD). The doses used were 100% (10000 ppm), 90% (9000 ppm), 80% (8000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). The results showed that the hot water extract of R. apiculata leaves at a dose of 1000-10000 ppm gave various inhibitory diameters ranging from 6.25-10.08 mm and the clear zone formed was classified as medium

Guava leaves can be utilized as feed additives and medicine for aquaculture. Limiting factors in aquaculture, including stocking density, and salinity can cause stress in fish, so they are easily infected with disease. This study was conducted to determine the effect of guava on the blood glucose of carp reared in brackish water media. This research was conducted from August to September 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The method used in this study is an experimental method using a completely randomized design with 5 (five) treatments and 3 (three) replicates, the treatments are T0 (control), T1 (1.0g), T2 (1.5g), T3 (2 g), and T4 (2.5g). Carp measuring 5.00 ± 1.00 cm with a weight of 4.00 ± 0.50 g were obtained from farmers in the Koto Panjang hydroelectric reservoir, Sumatra, Indonesia. Fish rearing was carried out for 60 days. The results showed that the provision of guava leaves in the feed affected the blood glucose levels of carp. The dose of 1.5 g/100g feed (T2) was the optimal dose in increasing the blood glucose of carp reared in brackish water media and infected with A. hydrophila bacteria

Background: Strategies to increase body resistance and prevent disease in aquaculture include using vaccines, antibiotics, and probiotics. Today, the use of antibiotics with natural ingredients is becoming a trend. One of the natural ingredients that contain high antioxidants and antibiotics is Sargassum sp. Methods: This research was conducted from March to May 2022 at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau, in two stages: 1) the sensitivity of extracts of Sargassum sp. and 2) the application of Sargassum sp. extract orally in tilapia (O. niloticus). The parameters measured were clear zone, minimum inhibitory concentration, LD50 test of leaf extract of Sargassum sp. in tilapia (O. niloticus), hemoglobin levels, hematocrit, total leukocytes, total erythrocytes, leukocyte differentiation, and survival rate. Data on hematology parameters were tabulated and analyzed using a One-Way ANOVA followed by a Student Newman Keuls (SNK) test when deemed necessary. Results: The results showed that the extract of Sargassum sp. inhibited the growth of Aeromonas hydrophila bacteria with a clear zone of 6.5-15.0 mm, which is classified as resistant. At doses of 2000, 2500, and 3000 ppm, it did not cause death in fish for 96 hours (LD50). Hematological parameters can be a sign of the health status of fish. Tilapia given Sargassum sp. in different doses gave an effect between treatments, both after 30 days of rearing and post-test against A. hydrophila bacteria (p<0.05). The results showed that the hematology of fish fed with Sargassum sp. extract was in the normal or healthy range. Healthy tilapia had erythrocyte counts ranging from 1.34-2.11×106 cells/mm3, hematocrit 26.17-33.19%, hemoglobin 6.26-11.2 g/dL and total leukocytes 1.01-1.50×104 cells/mm3 and total erythrocytes 5.88-9.13×104 cells/ mm3. Conclusions: A dose of 3000 ppm provided the highest health improvement against A. hydrophila bacterial infection.

Chaetomorpha sp is a type of green marcoalgae that is rich in bioactive compound that function as antibacterial, anti-inflammatory and can increase fish immunity. This study aims to determine the immunostimulating effect of Chaetomorpha sp macroalgae extract on the leukocyte profile of Tilapia infected with A. hydrophila. The experimental method used a completely randomized design (CRD) with four extract dose treatments (0, 25, 50, 75 mL/kg feed) and three replicates. The test fish used were 2.63 ± 0.26g, reared in black tanks with a size of 60x30x30 cm, a volume of 80 L with a stocking density of 1 fish/4 L, and a recirculation system. Maintenance was carried out for 60 days with a frequency of feeding three times a day, namely 08.00 AM, 01.00 PM, and 05.00 PM, as much as 5% of body weight. The parameters observed were total leukocytes, leukocyte differentiation, and phagocytosis index. The results showed that the administration of Chaetomorpha sp extract significantly increased total leukocytes, lymphocyte differentiation, and phagocytosis index (p0.05). The dose of 50 mL/kg feed is the optimal dose that affects the leukocyte profile of Tilapia. T0 (without extract) experienced 100% mortality, while the treatment group showed increased resistance to infection. Bioactive compounds such as sulfated polysaccharides and flavonoids in Chaetomorpha sp are thought to play a role in stimulating the innate immune system. This study highlights the potential of Chaetomorpha sp as an alternative to antibiotics in sustainable tilapia aquaculture.