Brain ischaemia (stroke) triggers an intense inflammatory response predominately mediated by the accumulation of inflammatory cells and mediators in the ischaemic brain. In this context, regulatory T (Treg) cells, a subpopulation of CD4⁺ T cells with immunosuppressive and anti‐inflammatory properties, are activated in the late stages of the disease. To date, the potential therapeutic usefulness of Treg cells has not been tested. In this study, we aimed to investigate whether Treg cells exert protection/repair following stroke. Both the adoptive transfer of Treg cells into ischaemic rats and the stimulation of endogenous T‐cell proliferation using a CD28 superagonist reduced the infarct size at 3–28 days following the ischaemic insult. Moreover, T cell‐treated animals had higher levels of FoxP3 and lower levels of IL‐1β, CD11b+ and CD68+ cells in the infarcted hemisphere when compared with control animals. However, T‐cell treatment did not alter the rate of proliferation of NeuN‐, NCAM‐ or CD31‐positive cells, thereby ruling out neurogenesis and angiogenesis in protection. These results suggest that adoptive transfer of T cells is a promising therapeutic strategy against the neurological consequences of stroke.

Historically, the resolution of optical imaging systems was dictated by diffraction, and the Rayleigh criterion was long considered an unsurpassable limit. In superresolution microscopy, this limit is overcome by manipulating the emission properties of the object. However, in passive imaging, when sources are uncontrolled, reaching sub-Rayleigh resolution remains a challenge. Here, we implement a quantum-metrology-inspired approach for estimating the separation between two incoherent sources, achieving a sensitivity five orders of magnitude beyond the Rayleigh limit. Using a spatial mode demultiplexer, we examine scenes with bright and faint sources, through intensity measurements in the Hermite–Gauss basis. Analyzing sensitivity and accuracy over an extensive range of separations, we demonstrate the remarkable effectiveness of demultiplexing for sub-Rayleigh separation estimation. These results effectively render the Rayleigh limit obsolete for passive imaging.

Glutamate excitotoxicity, metabolic rate and inflammatory response have been associated to the deleterious effects of temperature during the acute phase of stroke. So far, the association of temperature with these mechanisms has been studied individually. However, the simultaneous study of the influence of temperature on these mechanisms is necessary to clarify their contributions to temperature-mediated ischemic damage. We used non-invasive Magnetic Resonance Spectroscopy to simultaneously measure temperature, glutamate excitotoxicity and metabolic rate in the brain in animal models of ischemia. The immune response to ischemia was measured through molecular serum markers in peripheral blood. We submitted groups of animals to different experimental conditions (hypothermia at 33°C, normothermia at 37°C and hyperthermia at 39°C), and combined these conditions with pharmacological modulation of glutamate levels in the brain through systemic injections of glutamate and oxaloacetate. We show that pharmacological modulation of glutamate levels can neutralize the deleterious effects of hyperthermia and the beneficial effects of hypothermia, however the analysis of the inflammatory response and metabolic rate, demonstrated that their effects on ischemic damage are less critical than glutamate excitotoxity. We conclude that glutamate excitotoxicity is the key molecular mechanism which is influenced by body temperature during the acute phase of brain stroke.

In the current quest for efficient and experimentally feasible platforms for implementation of multimode squeezing and entanglement in the continuous variable regime, we underpin and complement our results on the generation of versatile multimode entanglement and cluster states in nonlinear waveguide arrays presented by Barral et al., arXiv:1912.11154 [Phys. Rev. Appl. (to be published)]. We present detailed derivations of the equations that describe the propagation of light through this system, and then we focus on parameter regimes where these equations can be solved analytically. These analytical solutions build an intuition for the wide landscape of quantum states that are accessible through the activation of pumping, coupling, and measurement schemes. Furthermore, we showcase the acquired insights by using one of the identified analytical solutions to exhibit the generation, optimization, and scalability of spatial linear cluster states.

Triple-photon states generated by three-mode spontaneous parametric down-conversion are the paradigm of unconditional non-Gaussian states, essential assets for quantum advantage. How to fully characterize their non-Gaussian entanglement remains however elusive. We propose here a set of sufficient and necessary conditions for separability of the broad family of spontaneously generated three-mode non-Gaussian states. We further derive state-of-the-art conditions for genuine tripartite non-Gaussian entanglement, the strongest class of entanglement. We apply our criteria to triple-photon states revealing that they are fully inseparable and genuinely entangled in moments of order $3n$. Our results establish a systematic framework for characterizing the entanglement of triple-photon states and thus fostering their application in quantum information protocols.