Increasing energy demands and fossil fuel consumption causing global warming has motivated research to find alternative energy sources such as biofuels. Giant reed (Arundo donax L.), a lignocellulosic, perennial, rhizomatous grass has been proposed as an important bioenergy crop for advanced biofuel in the Mediterranean area. Anaerobic digestion for advanced biomethane seems to be a promising approach. However, the presence of lignin in lignocellulosic biomass represents the main obstacle to its production (due to its recalcitrance). Thus, to use effectively lignocellulosic biomass in anaerobic digestion, one or more pretreatment steps are needed to aid microorganisms access to the plant cell wall. To this end, the present study investigated the effect of fungal pretreatment of giant reeds obtained from two different harvesting time (autumn and winter) on biomethane production by anaerobic digestion using two white rot fungi (Pleurotus ostreatus and Irpex lactus, respectively). The highest biomass lignin degradation after 30 days incubation with P. ostreatus in both autumn (27.1%) and winter (31.5%) harvest time. P. ostreatus pretreatment showed promising results for anaerobic digestion of giant reed achieving a cumulative yield of 130.9 NmL g⁻¹ VS for the winter harvest, whereas I. lacteus showed a decrease in methane yield as compared with the untreated biomass (77.4 NmL g⁻¹ VS and 73.3 NmL g⁻¹ VS for winter and autumn harvest, respectively). I. lacteus pretreatment resulted in a loss of both holocellulose and lignin, indicating that this strain was less selective than P. ostreatus. Further studies are necessary to identify white rot fungi more suitable to lignocellulosic biomass and optimize biological pretreatment conditions to reduce its duration.

Castor (Ricinus communis L.) is an oilseed species that can be grown as a semi-perennial in Mediterranean environments, including the coastal areas of Sicily. The present study investigated the optimization of cultivation techniques for castor, with the crop being maintained over a two-year period, through the evaluation of different agronomical inputs in order to increase seed yield. The effects of irrigation (I) and nitrogen fertilization (N) on the seed and oil yield and their components were assessed in castor cultivated in a typical semi-arid environment. Four levels of irrigation (I0, I30, I60, and I100: 0, 30, 60, and 100% of crop evapotranspiration—ETm restoration, respectively) as the main plot and three levels of nitrogen fertilization (N) (0, 60, and 120 kg N ha⁻¹) as the sub-plot were considered. Irrigation mostly affected the number of racemes per plant, the number of capsules per raceme, and the seed weight. The oil content was, on average, 39.2% and 45.6% for the first and second year, respectively. The highest seed yield was obtained by I100N120 treatment (4154.0 kg ha⁻¹⁾; however, the combination of a high soil nitrogen level (N120) and medium water availability (I60) resulted in satisfactory seed and oil yields. The reduction in the irrigation water to an intermediate level could be also an environmentally friendly strategy not significantly affecting yields.

Raising water and energy productivity in agriculture can contribute to reducing the pressure on the limited freshwater availability and non-renewable energy sources. Bioenergy perennial grasses are efficient from a water perspective and can afford a low-energy cultivation system; however, crop selection and cultivation practices for minimizing land use change and maximizing resource use efficiencies remain a challenging task in view of sustainable bioeconomy development. The present work investigated the soil water effect on a long-term plantation of Saccharum (Saccharum spontaneum ssp. aegyptiacum), a bioenergy perennial grass holding great promise for semiarid Mediterranean areas. The plantation was in its 13th year following establishment and was subjected to three levels of irrigation for three successive growing seasons. Regression models between crop water use (CWU) and productivity, biomass composition, energy, and water indicators showed different prediction curves. Raising CWU (from 230 to 920 mm) enhanced the dry biomass yield (from 14.8 to 30.1 Mg ha⁻¹) and the net energy value (from 257.6 to 511 GJ ha⁻¹). On the same CWU range, unirrigated crops improved the energy efficiency (from 99.8 to 58.5 GJ ha⁻¹), the energy productivity (from 5.6 to 3.4 Mg GJ⁻¹) and the water productivity (from 114.5 to 56.1 MJ m⁻³) by reducing the water footprint (from 8.7 to 17.8 m³ GJ⁻¹). Biomass composition was also superior in unirrigated crops, as the lower heating value, structural polysaccharides, and the acid detergent lignin were higher, while ash and soluble compounds were lower. Present findings demonstrated the good yield levels and persistence of Saccharum, improving our knowledge of plant responses to changing soil water availability to maximize energy and conserve natural resources, paving the way for sustainable bioeconomy development in the Mediterranean area.