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Dr. Kumar Saurav
Institute of Microbiology of the CAS, v. v. i.

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0 Drug Discovery
0 quorum sensing
0 natural product
0 Cyanobacterial Toxins
0 Antivirulence therapy

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Article
Published: 25 August 2021 in mSphere
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The regulation of the production of cyanopeptides beyond microcystin is essential to understand their ecological role in complex microbial communities, e.g., harmful cyanobacterial blooms. The role of chemical communication between the cyanobacterium and the epibionts within its phycosphere is at an initial stage of research, and little is understood about its specificity.

ACS Style

Subhasish Saha; Paul-Adrian Bulzu; Petra Urajová; Jan Mareš; Grzegorz Konert; João Câmara Manoel; Markéta Macho; Daniela Ewe; Pavel Hrouzek; Jiří Masojídek; Rohit Ghai; Kumar Saurav. Quorum-Sensing Signals from Epibiont Mediate the Induction of Novel Microviridins in the Mat-Forming Cyanobacterial Genus Nostoc. mSphere 2021, 6, e0056221 .

AMA Style

Subhasish Saha, Paul-Adrian Bulzu, Petra Urajová, Jan Mareš, Grzegorz Konert, João Câmara Manoel, Markéta Macho, Daniela Ewe, Pavel Hrouzek, Jiří Masojídek, Rohit Ghai, Kumar Saurav. Quorum-Sensing Signals from Epibiont Mediate the Induction of Novel Microviridins in the Mat-Forming Cyanobacterial Genus Nostoc. mSphere. 2021; 6 (4):e0056221.

Chicago/Turabian Style

Subhasish Saha; Paul-Adrian Bulzu; Petra Urajová; Jan Mareš; Grzegorz Konert; João Câmara Manoel; Markéta Macho; Daniela Ewe; Pavel Hrouzek; Jiří Masojídek; Rohit Ghai; Kumar Saurav. 2021. "Quorum-Sensing Signals from Epibiont Mediate the Induction of Novel Microviridins in the Mat-Forming Cyanobacterial Genus Nostoc." mSphere 6, no. 4: e0056221.

Article
Published: 11 August 2021 in Applied and Environmental Microbiology
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All living organisms depend on iron as an essential cofactor for indispensable enzymes. However, the sources of bioavailable iron are often limited.

ACS Style

Tomáš Galica; Nicola Borbone; Jan Mareš; Andreja Kust; Alessia Caso; Germana Esposito; Kumar Saurav; Jan Hájek; Klára Řeháková; Petra Urajová; Valeria Costantino; Pavel Hrouzek. Cyanochelins, an Overlooked Class of Widely Distributed Cyanobacterial Siderophores, Discovered by Silent Gene Cluster Awakening. Applied and Environmental Microbiology 2021, 87, 1 .

AMA Style

Tomáš Galica, Nicola Borbone, Jan Mareš, Andreja Kust, Alessia Caso, Germana Esposito, Kumar Saurav, Jan Hájek, Klára Řeháková, Petra Urajová, Valeria Costantino, Pavel Hrouzek. Cyanochelins, an Overlooked Class of Widely Distributed Cyanobacterial Siderophores, Discovered by Silent Gene Cluster Awakening. Applied and Environmental Microbiology. 2021; 87 (17):1.

Chicago/Turabian Style

Tomáš Galica; Nicola Borbone; Jan Mareš; Andreja Kust; Alessia Caso; Germana Esposito; Kumar Saurav; Jan Hájek; Klára Řeháková; Petra Urajová; Valeria Costantino; Pavel Hrouzek. 2021. "Cyanochelins, an Overlooked Class of Widely Distributed Cyanobacterial Siderophores, Discovered by Silent Gene Cluster Awakening." Applied and Environmental Microbiology 87, no. 17: 1.

Preprint content
Published: 24 April 2021
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The regulation of oligopeptides production is essential in understanding their ecological role in complex microbial communities including harmful cyanobacterial blooms. The role of chemical communication between the cyanobacterium and the microbial community harboured as epibionts within its phycosphere is at an initial stage of research and little is understood about its specificity. Herein, we present insight into the role of a bacterial epibiont in regulating production of cyanobacterial oligopeptides microviridins, well-known elastase inhibitors with presumed anti-grazing effects, in an ecologically important cyanobacterial genus Nostoc. Heterologous expression and identification of specific signal molecules from the epibiont suggest the role of a quorum sensing-based interaction. Further, physiological experiments show an increase in microviridin production without affecting cyanobacterial growth and photosynthetic activity. Simultaneously, oligopeptides presenting a selective inhibition pattern provide support for their specific function in response to the presence of cohabitant epibionts. Thus, the chemical interaction revealed in our study provides an example of an interspecies signalling pathway monitoring the bacterial flora around the cyanobacterial filaments and induction of intrinsic species-specific metabolic responses. IMPORTANCE The regulation of cyanopeptide production beyond microcystin is essential to understand their ecological role in complex microbial communities, e.g. harmful cyanobacterial blooms. The role of chemical communication between the cyanobacterium and the epibionts within its phycosphere is at an initial stage of research and little is understood about its specificity. The frequency of cyanopeptide occurrence also demonstrates the need to understand the contribution of cyanobacterial peptides to the overall biological impact of cyanopeptides on aquatic organisms and vertebrates including humans. Our results shed light on the epibiont control of cyanopeptide production via quorum sensing mechanisms and we posit that such mechanisms may be widespread in natural cyanobacterial bloom community regulation.

ACS Style

Subhasish Saha; Paul-Adrian Bulzu; Petra Urajová; Jan Mareš; Grzegorz Konert; João Câmara Manoel; Markéta Macho; Daniela Ewe; Pavel Hrouzek; Jiří Masojídek; Rohit Ghai; Kumar Saurav. Quorum sensing signals from epibiont mediate the induction of bioactive peptides in mat-forming cyanobacteria Nostoc. 2021, 1 .

AMA Style

Subhasish Saha, Paul-Adrian Bulzu, Petra Urajová, Jan Mareš, Grzegorz Konert, João Câmara Manoel, Markéta Macho, Daniela Ewe, Pavel Hrouzek, Jiří Masojídek, Rohit Ghai, Kumar Saurav. Quorum sensing signals from epibiont mediate the induction of bioactive peptides in mat-forming cyanobacteria Nostoc. . 2021; ():1.

Chicago/Turabian Style

Subhasish Saha; Paul-Adrian Bulzu; Petra Urajová; Jan Mareš; Grzegorz Konert; João Câmara Manoel; Markéta Macho; Daniela Ewe; Pavel Hrouzek; Jiří Masojídek; Rohit Ghai; Kumar Saurav. 2021. "Quorum sensing signals from epibiont mediate the induction of bioactive peptides in mat-forming cyanobacteria Nostoc." , no. : 1.

Brief report
Published: 07 January 2021 in Microbiology Resource Announcements
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We report the draft genome sequence of Streptomyces sp. strain VITNK9, isolated from a soil sample collected in Vellore District (12.9165°N, 79.1325°E), Tamil Nadu, India, with an assembly size of 7,920,076 bp and 72.7% GC content.

ACS Style

Subhasish Saha; Nabila Mohammad Ishaque; Ilia Burgsdorf; Markéta Macho; Daniela Ewe; Laura Steindler; Pavel Hrouzek; Krishnan Kannabiran; Kumar Saurav. Draft Genome Sequence of Terrestrial Streptomyces sp. Strain VITNK9, Isolated from Vellore, Tamil Nadu, India, Exhibiting Antagonistic Activity against Fish Pathogens. Microbiology Resource Announcements 2021, 10, 1 .

AMA Style

Subhasish Saha, Nabila Mohammad Ishaque, Ilia Burgsdorf, Markéta Macho, Daniela Ewe, Laura Steindler, Pavel Hrouzek, Krishnan Kannabiran, Kumar Saurav. Draft Genome Sequence of Terrestrial Streptomyces sp. Strain VITNK9, Isolated from Vellore, Tamil Nadu, India, Exhibiting Antagonistic Activity against Fish Pathogens. Microbiology Resource Announcements. 2021; 10 (1):1.

Chicago/Turabian Style

Subhasish Saha; Nabila Mohammad Ishaque; Ilia Burgsdorf; Markéta Macho; Daniela Ewe; Laura Steindler; Pavel Hrouzek; Krishnan Kannabiran; Kumar Saurav. 2021. "Draft Genome Sequence of Terrestrial Streptomyces sp. Strain VITNK9, Isolated from Vellore, Tamil Nadu, India, Exhibiting Antagonistic Activity against Fish Pathogens." Microbiology Resource Announcements 10, no. 1: 1.

Review
Published: 02 November 2020 in Foods
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Xylitol is a white crystalline, amorphous sugar alcohol and low-calorie sweetener. Xylitol prevents demineralization of teeth and bones, otitis media infection, respiratory tract infections, inflammation and cancer progression. NADPH generated in xylitol metabolism aid in the treatment of glucose-6-phosphate deficiency-associated hemolytic anemia. Moreover, it has a negligible effect on blood glucose and plasma insulin levels due to its unique metabolism. Its diverse applications in pharmaceuticals, cosmetics, food and polymer industries fueled its market growth and made it one of the top 12 bio-products. Recently, xylitol has also been used as a drug carrier due to its high permeability and non-toxic nature. However, it become a challenge to fulfil the rapidly increasing market demand of xylitol. Xylitol is present in fruit and vegetables, but at very low concentrations, which is not adequate to satisfy the consumer demand. With the passage of time, other methods including chemical catalysis, microbial and enzymatic biotransformation, have also been developed for its large-scale production. Nevertheless, large scale production still suffers from high cost of production. In this review, we summarize some alternative approaches and recent advancements that significantly improve the yield and lower the cost of production.

ACS Style

Vishal Ahuja; Markéta Macho; Daniela Ewe; Manoj Singh; Subhasish Saha; Kumar Saurav. Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism. Foods 2020, 9, 1592 .

AMA Style

Vishal Ahuja, Markéta Macho, Daniela Ewe, Manoj Singh, Subhasish Saha, Kumar Saurav. Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism. Foods. 2020; 9 (11):1592.

Chicago/Turabian Style

Vishal Ahuja; Markéta Macho; Daniela Ewe; Manoj Singh; Subhasish Saha; Kumar Saurav. 2020. "Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism." Foods 9, no. 11: 1592.

Journal article
Published: 31 August 2020 in Toxins
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Man-made shallow fishponds in the Czech Republic have been facing high eutrophication since the 1950s. Anthropogenic eutrophication and feeding of fish have strongly affected the physicochemical properties of water and its aquatic community composition, leading to harmful algal bloom formation. In our current study, we characterized the phytoplankton community across three eutrophic ponds to assess the phytoplankton dynamics during the vegetation season. We microscopically identified and quantified 29 cyanobacterial taxa comprising non-toxigenic and toxigenic species. Further, a detailed cyanopeptides (CNPs) profiling was performed using molecular networking analysis of liquid chromatography-tandem mass spectrometry (LC-MS/MS) data coupled with a dereplication strategy. This MS networking approach, coupled with dereplication, on the online global natural product social networking (GNPS) web platform led us to putatively identify forty CNPs: fourteen anabaenopeptins, ten microcystins, five cyanopeptolins, six microginins, two cyanobactins, a dipeptide radiosumin, a cyclooctapeptide planktocyclin, and epidolastatin 12. We applied the binary logistic regression to estimate the CNPs producers by correlating the GNPS data with the species abundance. The usage of the GNPS web platform proved a valuable approach for the rapid and simultaneous detection of a large number of peptides and rapid risk assessments for harmful blooms.

ACS Style

Andreja Kust; Klára Řeháková; Jaroslav Vrba; Vincent Maicher; Jan Mareš; Pavel Hrouzek; Maria-Cecilia Chiriac; Zdeňka Benedová; Blanka Tesařová; Kumar Saurav. Insight into Unprecedented Diversity of Cyanopeptides in Eutrophic Ponds Using an MS/MS Networking Approach. Toxins 2020, 12, 561 .

AMA Style

Andreja Kust, Klára Řeháková, Jaroslav Vrba, Vincent Maicher, Jan Mareš, Pavel Hrouzek, Maria-Cecilia Chiriac, Zdeňka Benedová, Blanka Tesařová, Kumar Saurav. Insight into Unprecedented Diversity of Cyanopeptides in Eutrophic Ponds Using an MS/MS Networking Approach. Toxins. 2020; 12 (9):561.

Chicago/Turabian Style

Andreja Kust; Klára Řeháková; Jaroslav Vrba; Vincent Maicher; Jan Mareš; Pavel Hrouzek; Maria-Cecilia Chiriac; Zdeňka Benedová; Blanka Tesařová; Kumar Saurav. 2020. "Insight into Unprecedented Diversity of Cyanopeptides in Eutrophic Ponds Using an MS/MS Networking Approach." Toxins 12, no. 9: 561.

Journal article
Published: 20 August 2020 in Molecules
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Heterocytous cyanobacteria are among the most prolific sources of bioactive secondary metabolites, including anabaenopeptins (APTs). A terrestrial filamentous Brasilonema sp. CT11 collected in Costa Rica bamboo forest as a black mat, was studied using a multidisciplinary approach: genome mining and HPLC-HRMS/MS coupled with bioinformatic analyses. Herein, we report the nearly complete genome consisting of 8.79 Mbp with a GC content of 42.4%. Moreover, we report on three novel tryptophan-containing APTs; anabaenopeptin 788 (1), anabaenopeptin 802 (2), and anabaenopeptin 816 (3). Furthermore, the structure of two homologues, i.e., anabaenopeptin 802 (2a) and anabaenopeptin 802 (2b), was determined by spectroscopic analysis (NMR and MS). Both compounds were shown to exert weak to moderate antiproliferative activity against HeLa cell lines. This study also provides the unique and diverse potential of biosynthetic gene clusters and an assessment of the predicted chemical space yet to be discovered from this genus.

ACS Style

Subhasish Saha; Germana Esposito; Petra Urajová; Jan Mareš; Daniela Ewe; Alessia Caso; Markéta Macho; Kateřina Delawská; Andreja Kust; Pavel Hrouzek; Josef Juráň; Valeria Costantino; Kumar Saurav. Discovery of Unusual Cyanobacterial Tryptophan-Containing Anabaenopeptins by MS/MS-Based Molecular Networking. Molecules 2020, 25, 3786 .

AMA Style

Subhasish Saha, Germana Esposito, Petra Urajová, Jan Mareš, Daniela Ewe, Alessia Caso, Markéta Macho, Kateřina Delawská, Andreja Kust, Pavel Hrouzek, Josef Juráň, Valeria Costantino, Kumar Saurav. Discovery of Unusual Cyanobacterial Tryptophan-Containing Anabaenopeptins by MS/MS-Based Molecular Networking. Molecules. 2020; 25 (17):3786.

Chicago/Turabian Style

Subhasish Saha; Germana Esposito; Petra Urajová; Jan Mareš; Daniela Ewe; Alessia Caso; Markéta Macho; Kateřina Delawská; Andreja Kust; Pavel Hrouzek; Josef Juráň; Valeria Costantino; Kumar Saurav. 2020. "Discovery of Unusual Cyanobacterial Tryptophan-Containing Anabaenopeptins by MS/MS-Based Molecular Networking." Molecules 25, no. 17: 3786.

Preprint
Published: 12 August 2020
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Man-made shallow fishponds in the Czech Republic have been facing a high eutrophication since 1950s. Anthropogenic eutrophication and feeding of fish have strongly affected the physico-chemical properties of water and its aquatic community composition leading to harmful algal bloom formation. In our current study, we have characterised the phytoplankton community across three hypertrophic ponds to assess the phytoplankton dynamics during the vegetation season. We microscopically identified and quantified 29 cyanobacterial taxa comprised of non-toxigenic and toxigenic species. Further, a detailed cyanopeptides (CNPs) profiling was performed using molecular networking analysis of liquid chromatography tandem mass spectrometry (LC–MS/MS) data coupled with dereplication strategy. This MS networking approach coupled with dereplication on online global natural product social networking (GNPS) web platform led us to putatively identify forty CNPs: fourteen anabaenopeptins, ten microcystins, five cyanopeptolins, six microginins, two cyanobactins, a dipeptide radiosumin, a cyclooctapeptide planktocyclin and epidolastatin12. We have applied the binary logistic regression to estimate the CNPs producer by correlating the GNPS data with the species abundance. Usage of The combination of molecular networking and dereplication on online global natural product social networking (GNPS) web platform has proved as a valuable approach for rapid and simultaneous detection of high number of peptides, and rapidly assessing the risk for harmful bloom.

ACS Style

Andreja Kust; Klára Řeháková; Jaroslav Vrba; Vincent Maicher; Jan Mareš; Pavel Hrouzek; Cecilia Chiriac; Zdeňka Benedová; Blanka Tesařová; Kumar Saurav. Insight Into Unprecedented Diversity of Cyanopeptides in Eutrophic Ponds Using a MS/MS Networking Approach. 2020, 1 .

AMA Style

Andreja Kust, Klára Řeháková, Jaroslav Vrba, Vincent Maicher, Jan Mareš, Pavel Hrouzek, Cecilia Chiriac, Zdeňka Benedová, Blanka Tesařová, Kumar Saurav. Insight Into Unprecedented Diversity of Cyanopeptides in Eutrophic Ponds Using a MS/MS Networking Approach. . 2020; ():1.

Chicago/Turabian Style

Andreja Kust; Klára Řeháková; Jaroslav Vrba; Vincent Maicher; Jan Mareš; Pavel Hrouzek; Cecilia Chiriac; Zdeňka Benedová; Blanka Tesařová; Kumar Saurav. 2020. "Insight Into Unprecedented Diversity of Cyanopeptides in Eutrophic Ponds Using a MS/MS Networking Approach." , no. : 1.

Preprint
Published: 23 July 2020
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Heterocytous cyanobacteria are among the most prolific source of bioactive secondary metabolites, including anabaenopeptins (APTs). A terrestrial filamentous Brasilonema sp. CT11 collected in Costa Rica bamboo forest, as black mat was studied using a multidisciplinary approach: genome mining and HPLC-HRMS/MS coupled with bionformatic analyses. Herein, we report the nearly complete genome consisting 8.79 Mbp with a GC content of 42.4%. Moreover, we report on three novel tryptophane-containing APTs; anabaenopeptin 788 (1), anabaenopeptin 802 (2) and anabaenopeptin 816 (3). Further, the structure of two homologues, i.e., anabaenopeptin 802 (2a) and anabaenopeptin 802 (2b) was determined by spectroscopic analysis (NMR and MS). Both compounds were shown to exert weak to moderate antiproliferative activity against HeLa cell lines. This study also provides the unique and diverse potential of biosynthetic gene clusters and an assessment of the predicted chemical space yet to be discovered from this genus.

ACS Style

Subhasish Saha; Germana Esposito; Petra Urajova; Jan Mareš; Daniela Ewe; Alessia Caso; Marketa Macho; Katerina Delawska; Andreja Kust; Pavel Hrouzek; Josef Juran; Valeria Costantino; Kumar Saurav. Discovery of Unusual Cyanobacterial Tryptophan-containing Anabaenopeptins by MS/MS Based Molecular Networking. 2020, 1 .

AMA Style

Subhasish Saha, Germana Esposito, Petra Urajova, Jan Mareš, Daniela Ewe, Alessia Caso, Marketa Macho, Katerina Delawska, Andreja Kust, Pavel Hrouzek, Josef Juran, Valeria Costantino, Kumar Saurav. Discovery of Unusual Cyanobacterial Tryptophan-containing Anabaenopeptins by MS/MS Based Molecular Networking. . 2020; ():1.

Chicago/Turabian Style

Subhasish Saha; Germana Esposito; Petra Urajova; Jan Mareš; Daniela Ewe; Alessia Caso; Marketa Macho; Katerina Delawska; Andreja Kust; Pavel Hrouzek; Josef Juran; Valeria Costantino; Kumar Saurav. 2020. "Discovery of Unusual Cyanobacterial Tryptophan-containing Anabaenopeptins by MS/MS Based Molecular Networking." , no. : 1.

Preprint content
Published: 18 April 2020
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Marine microbial communities vary seasonally and spatially, but these two factors are rarely addressed together. We studied temporal and spatial patterns of the microbial community structure and activity along a coast to offshore transect from the Israeli coast of the Eastern Mediterranean Sea (EMS) over six cruises, in three seasons of two consecutive years. The ultra-oligotrophic status of the South Eastern Mediterranean Sea was reflected in the microbial community composition that was dominated by oligotrophic microbial groups such as SAR11 throughout the year, even at the most coastal station sampled. Seasons affected microbial communities much more than distance from shore explaining about half of the observed variability in the microbial community, compared to only about 6% that was explained by station. However, the most coastal site differed significantly in community structure and activity from the three further offshore stations in early winter and summer, but not in spring. Our data on the microbial community composition and its seasonality from a transect into the South Eastern Levantine basin support the notion that the EMS behaves similar to open gyres rather than an inland sea.

ACS Style

Markus Haber; Dalit Roth-Rosenberg; Maya Lalzar; Ilia Burgsdorf; Kumar Saurav; Regina Lionheart; Yoav Lehahn; Dikla Aharonovich; Daniel Sher; Michael D Krom; Laura Steindler. Microbial communities in an ultra-oligotrophic sea are more affected by season than by distance from shore. 2020, 1 .

AMA Style

Markus Haber, Dalit Roth-Rosenberg, Maya Lalzar, Ilia Burgsdorf, Kumar Saurav, Regina Lionheart, Yoav Lehahn, Dikla Aharonovich, Daniel Sher, Michael D Krom, Laura Steindler. Microbial communities in an ultra-oligotrophic sea are more affected by season than by distance from shore. . 2020; ():1.

Chicago/Turabian Style

Markus Haber; Dalit Roth-Rosenberg; Maya Lalzar; Ilia Burgsdorf; Kumar Saurav; Regina Lionheart; Yoav Lehahn; Dikla Aharonovich; Daniel Sher; Michael D Krom; Laura Steindler. 2020. "Microbial communities in an ultra-oligotrophic sea are more affected by season than by distance from shore." , no. : 1.

Journal article
Published: 20 February 2020 in Marine Drugs
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Marine sponges, a well-documented prolific source of natural products, harbor highly diverse microbial communities. Their extracts were previously shown to contain quorum sensing (QS) signal molecules of the N-acyl homoserine lactone (AHL) type, known to orchestrate bacterial gene regulation. Some bacteria and eukaryotic organisms are known to produce molecules that can interfere with QS signaling, thus affecting microbial genetic regulation and function. In the present study, we established the production of both QS signal molecules as well as QS inhibitory (QSI) molecules in the sponge species Sarcotragus spinosulus. A total of eighteen saturated acyl chain AHLs were identified along with six unsaturated acyl chain AHLs. Bioassay-guided purification led to the isolation of two brominated metabolites with QSI activity. The structures of these compounds were elucidated by comparative spectral analysis of 1HNMR and HR-MS data and were identified as 3-bromo-4-methoxyphenethylamine (1) and 5,6-dibromo-N,N-dimethyltryptamine (2). The QSI activity of compounds 1 and 2 was evaluated using reporter gene assays for long- and short-chain AHL signals (Escherichia coli pSB1075 and E. coli pSB401, respectively). QSI activity was further confirmed by measuring dose-dependent inhibition of proteolytic activity and pyocyanin production in Pseudomonas aeruginosa PAO1. The obtained results show the coexistence of QS and QSI in S. spinosulus, a complex signal network that may mediate the orchestrated function of the microbiome within the sponge holobiont.

ACS Style

Kumar Saurav; Nicola Borbone; Ilia Burgsdorf; Roberta Teta; Alessia Caso; Rinat Bar-Shalom; Germana Esposito; Maya Britstein; Laura Steindler; Valeria Costantino. Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus. Marine Drugs 2020, 18, 127 .

AMA Style

Kumar Saurav, Nicola Borbone, Ilia Burgsdorf, Roberta Teta, Alessia Caso, Rinat Bar-Shalom, Germana Esposito, Maya Britstein, Laura Steindler, Valeria Costantino. Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus. Marine Drugs. 2020; 18 (2):127.

Chicago/Turabian Style

Kumar Saurav; Nicola Borbone; Ilia Burgsdorf; Roberta Teta; Alessia Caso; Rinat Bar-Shalom; Germana Esposito; Maya Britstein; Laura Steindler; Valeria Costantino. 2020. "Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus." Marine Drugs 18, no. 2: 127.

Journal article
Published: 16 January 2020 in Microorganisms
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Streptomyces are among the most promising genera in terms of production ability to biosynthesize a variety of bioactive secondary metabolites with pharmaceutical interest. Coinciding with the increase in genomic sequencing of these bacteria, mining of their genomes for biosynthetic gene clusters (BGCs) has become a routine component of natural product discovery. Herein, we describe the isolation and characterization of a Streptomyces tendae VITAKN with quorum sensing inhibitory (QSI) activity that was isolated from southern coastal part of India. The nearly complete genome consists of 8,621,231bp with a GC content of 72.2%. Sequence similarity networks of the BGCs detected from this strain against the Minimum Information about a Biosynthetic Gene Cluster (MIBiG) database and 3365 BGCs predicted by antiSMASH analysis of publicly available complete Streptomyces genomes were generated through the BiG-SCAPE-CORASON platform to evaluate its biosynthetic novelty. Crude extract analysis using high-performance liquid chromatography connected to high resolution tandem mass spectrometry (HPLC-HRMS/MS) and dereplication through the Global Natural Product Social Molecular Networking (GNPS) online workflow resulted in the identification of cyclic dipeptides (2, 5-diketopiperazines, DKPs) in the extract, which are known to possess QSI activity. Our results highlight the potential of genome mining coupled with LC-HRMS/MS and in silico tools (GNPS) as a valid approach for the discovery of novel QSI lead compounds. This study also provides the biosynthetic diversity of BGCs and an assessment of the predicted chemical space yet to be discovered.

ACS Style

Nabila Mohammed Ishaque; Ilia Burgsdorf; Jessie James Limlingan Malit; Subhasish Saha; Roberta Teta; Daniela Ewe; Krishnan Kannabiran; Pavel Hrouzek; Laura Steindler; Valeria Costantino; Kumar Saurav. Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India. Microorganisms 2020, 8, 121 .

AMA Style

Nabila Mohammed Ishaque, Ilia Burgsdorf, Jessie James Limlingan Malit, Subhasish Saha, Roberta Teta, Daniela Ewe, Krishnan Kannabiran, Pavel Hrouzek, Laura Steindler, Valeria Costantino, Kumar Saurav. Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India. Microorganisms. 2020; 8 (1):121.

Chicago/Turabian Style

Nabila Mohammed Ishaque; Ilia Burgsdorf; Jessie James Limlingan Malit; Subhasish Saha; Roberta Teta; Daniela Ewe; Krishnan Kannabiran; Pavel Hrouzek; Laura Steindler; Valeria Costantino; Kumar Saurav. 2020. "Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India." Microorganisms 8, no. 1: 121.

Preprint
Published: 31 December 2019
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Streptomyces, being one of the most promising genera due to its ability to synthesize a variety of bioactive secondary metabolites of pharmaceutical interest, here studied in relation to its genomic and metabolomic potential. Coinciding with the increase in sequenced data, mining of bacterial genomes for biosynthetic gene clusters (BGCs) has become a routine component of natural product discovery. Herein, we describe the isolation and characterization of a Streptomyces tendae VITAKN with quorum sensing inhibitory activity (QSI) that was isolated from southern coastal parts of India. The nearly complete genome consists of 8,621,231bp with a GC content of 72.2%. Utilizing the BiG-SCAPE-CORASON platform, a sequence similarity network predicted from this strain was evaluated through sequence similarity analysis with the MIBiG database and existing 3,365 BGCs predicted by antiSMASH analysis of publicly available complete Streptomyces genomes. Crude extract analyzed on LC-HRMS/MS and Global Natural Product Social Molecular Networking (GNPS) online workflow using dereplication resulted in the identification of cyclic dipeptides (2,5-diketopiperazines, DKPs) in the extract, which are known to possess QSI activity. Our results highlight the potential use of genomic mining coupled with LC-HRMS/MS and bionformatic tools (GNPS) as a potent approach for metabolome studies in discovering novel QSI lead compounds. This study also provides the biosynthetic diversity of these BGCs and an assessment of the predicted chemical space yet to be discovered.

ACS Style

Nabila Imthiyaz; Ilia Burgsdorf; Jessie James Limlingan Malit; Subhasish Saha; Roberta Teta; Daniela Ewe; Krishnan Kannabiran; Pavel Hrouzek; Laura Steindler; Valeria Costantino; Kumar Saurav. Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India. 2019, 1 .

AMA Style

Nabila Imthiyaz, Ilia Burgsdorf, Jessie James Limlingan Malit, Subhasish Saha, Roberta Teta, Daniela Ewe, Krishnan Kannabiran, Pavel Hrouzek, Laura Steindler, Valeria Costantino, Kumar Saurav. Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India. . 2019; ():1.

Chicago/Turabian Style

Nabila Imthiyaz; Ilia Burgsdorf; Jessie James Limlingan Malit; Subhasish Saha; Roberta Teta; Daniela Ewe; Krishnan Kannabiran; Pavel Hrouzek; Laura Steindler; Valeria Costantino; Kumar Saurav. 2019. "Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India." , no. : 1.

Conference paper
Published: 20 December 2019 in 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP
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Teleocidines originally found in japanese soil bacteria e. g. “Streptomyces mediocidicus” or “Streptomyces blastmyceticum” have aroused interest due to their ability of promoting tumor growth[1]. The structural feature which combines all these Teleocidin type substances is a 9-membered lactam ring linked to an indole nucleus[2]. It was reported that also Lyngbyatoxin, a secondary metabolite of the Hawaiian blue-green algae “Lyngbya Majuscula”, shows this structural feature[3]. Lyngbyatoxin is well-known for causing seaweed dermatitis to swimmers in Hawaii or Okinawa. Due to the structural similarity Lyngbyatoxin apparently was also proved to be a Tumor-promoter[1],[4]. We hereby report that “Streptomyces Blastmyceticum” can be induced to produce a slightly different spectrum of metabolites, by an altered cultivation scheme. Bacteria growth was performed in a very peptone rich medium for 4-5 days. LC / MS analysis of the culture solution showed presence of Lyngbyatoxin and other Teleocidin species. After centrifugation of the biomass, the supernatant solution was extracted with Amberlite XAD 1180N for 1 day. The crude extract was further purified by open normal phase and HPLC reversed phase chromatography. The LC / MS diagram of the purified compound can be seen in the figure below. The exact mass of Lyngbyatoxin was detected with less than 1 ppm error. Other Teleocidin compounds such as Olivoretin and Teleocidin B have been isolated in a similar manner. For further experiments other cultivation conditions will be tested on different strains of “Streptomyces” and the occurrence of Teleocidin compounds will be investigated.

ACS Style

S Bieringer; C Urmann; Kumar Saurav; P Hrouzek; H Riepl. Isolation of Lyngbyatoxin and other Teleocidin species from, Streptomyces Blastmyceticum” culture. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 2019, 85, P-459 .

AMA Style

S Bieringer, C Urmann, Kumar Saurav, P Hrouzek, H Riepl. Isolation of Lyngbyatoxin and other Teleocidin species from, Streptomyces Blastmyceticum” culture. 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. 2019; 85 (18):P-459.

Chicago/Turabian Style

S Bieringer; C Urmann; Kumar Saurav; P Hrouzek; H Riepl. 2019. "Isolation of Lyngbyatoxin and other Teleocidin species from, Streptomyces Blastmyceticum” culture." 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP 85, no. 18: P-459.

Preprint
Published: 13 December 2019
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Marine sponges, a well documented prolific source of natural products, harbors numerous microbial communities believed to possess N-acyl homoserine lactones (AHLs) mediated Quorum sensing (QS) as one of the mechanisms of interaction. Bacteria and eukaryotic organisms are known to produce molecules that can interfere with QS signaling, thus affecting microbial genetic regulation and function. In the present study, we established the potential for production of both QS signal molecules as well as QS interfering molecules (QSI) in the same sponge species Sarcotragus spinosulus. A total of eighteen saturated acyl chain AHLs were identified along with six putative unsaturated acyl chain AHLs. Bioassay guided purification led to the isolation of two brominated metabolites with QS-interfering activity. The structures of these compounds were elucidated by comparative spectral analysis of 1HNMR and HR-MS data and was identified as 3-Br-N-methyltyramine (1) and 5,6-dibromo-N,N-dimethyltryptamine (2). The QSI activity of compounds 1 and 2 were evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075 and E. coli pSB401) and was confirmed by measuring dose dependent inhibition of proteolytic activity and pyocyanin production in P. aeruginosa PAO1. The obtained results showed the co-existence of QS and QSI in S. spinosulus, a complex network which may mediate the orchestrated function of the microbiome within the sponge holobiont.

ACS Style

Kumar Saurav; Nicola Borbone; Ilia Burgsdorf; Roberta Teta; Alessia Caso; Rinat Bar-Shalom; Germana Esposito; Maya Britstein; Laura Steindler; Valeria Costantino. Co-existence of Quorum Sensing and Quorum Sensing Inhibitory Compounds in Marine Sponge Sarcotragus spinosulus. 2019, 1 .

AMA Style

Kumar Saurav, Nicola Borbone, Ilia Burgsdorf, Roberta Teta, Alessia Caso, Rinat Bar-Shalom, Germana Esposito, Maya Britstein, Laura Steindler, Valeria Costantino. Co-existence of Quorum Sensing and Quorum Sensing Inhibitory Compounds in Marine Sponge Sarcotragus spinosulus. . 2019; ():1.

Chicago/Turabian Style

Kumar Saurav; Nicola Borbone; Ilia Burgsdorf; Roberta Teta; Alessia Caso; Rinat Bar-Shalom; Germana Esposito; Maya Britstein; Laura Steindler; Valeria Costantino. 2019. "Co-existence of Quorum Sensing and Quorum Sensing Inhibitory Compounds in Marine Sponge Sarcotragus spinosulus." , no. : 1.

Rapid communication
Published: 28 October 2019 in Organic Letters
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Two polyketides neoenterocins A (1) and B (2), featuring a neighboring dicarbonyl motif and a furan-containing 5/6 ring system, were isolated from the enterocin producer Streptomyces sp. SCSIO 11863. Heterologous expression, gene disruptions, and isotope feeding experiments indicated that 1 and 2 were derived from the enterocin biosynthetic gene cluster. However, 2 was demonstrated as an artifact from enterocin via a unique skeleton rearrangement.

ACS Style

Liujuan Zheng; Xiaodong Jiang; Qingbo Zhang; Yiguang Zhu; Haibo Zhang; Wenjun Zhang; Kumar Saurav; Jinsong Liu; Changsheng Zhang. Discovery and Biosynthesis of Neoenterocins Indicate a Skeleton Rearrangement of Enterocin. Organic Letters 2019, 21, 9066 -9070.

AMA Style

Liujuan Zheng, Xiaodong Jiang, Qingbo Zhang, Yiguang Zhu, Haibo Zhang, Wenjun Zhang, Kumar Saurav, Jinsong Liu, Changsheng Zhang. Discovery and Biosynthesis of Neoenterocins Indicate a Skeleton Rearrangement of Enterocin. Organic Letters. 2019; 21 (22):9066-9070.

Chicago/Turabian Style

Liujuan Zheng; Xiaodong Jiang; Qingbo Zhang; Yiguang Zhu; Haibo Zhang; Wenjun Zhang; Kumar Saurav; Jinsong Liu; Changsheng Zhang. 2019. "Discovery and Biosynthesis of Neoenterocins Indicate a Skeleton Rearrangement of Enterocin." Organic Letters 21, no. 22: 9066-9070.

Original article
Published: 05 August 2019 in Folia Microbiologica
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The rapid emergence of resistance in pathogenic bacteria together with a steep decline in economic incentives has rendered a new wave in the drug development by the pharmaceutical industry and researchers. Since cyanobacteria are recognized as wide producers of pharmaceutically important compounds, we investigated thirty-four cyanobacterial extracts prepared by solvents of different polarities for their antimicrobial potential. Almost all tested cyanobacterial strains exhibited some degree of antimicrobial bioactivity, with more general effect on fungal strains compared with bacteria. Surprisingly ~50% of cyanobacterial extracts exhibited specific activity against one or few bacterial indicator strains with Gram-positive bacteria being more affected. Extracts of two most promising strains were subjected to activity-guided fractionation and determination of the minimum inhibitory concentration (MIC) against selected bacterial and fungal isolates. Multiple fractions were responsible for their antimicrobial effect with MIC reaching low-micromolar concentrations and in some of them high level of specificity was recorded. Twenty-six bioactive fractions analyzed on LC-HRMS/MS and Global Natural Product Social Molecular Networking (GNPS) online workflow using dereplication resulted in identification of only forty-nine peptide spectrum matches (PSMs) with eleven unique metabolites spectrum matches (MSMs). Interestingly, only three fractions from Nostoc calcicola Lukešová 3/97 and four fractions from Desmonostoc sp. Cc2 showed the presence of unique MSMs suggesting the presence of unknown antimicrobial metabolites among majority of bioactive fractions from both the strains. Our results highlight potential for isolation and discovery of potential antimicrobial bioactive lead molecules from cyanobacterial extracts.

ACS Style

Kumar Saurav; Markéta Macho; Andreja Kust; Kateřina Delawská; Jan Hájek; Pavel Hrouzek. Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking. Folia Microbiologica 2019, 64, 645 -654.

AMA Style

Kumar Saurav, Markéta Macho, Andreja Kust, Kateřina Delawská, Jan Hájek, Pavel Hrouzek. Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking. Folia Microbiologica. 2019; 64 (5):645-654.

Chicago/Turabian Style

Kumar Saurav; Markéta Macho; Andreja Kust; Kateřina Delawská; Jan Hájek; Pavel Hrouzek. 2019. "Antimicrobial activity and bioactive profiling of heterocytous cyanobacterial strains using MS/MS-based molecular networking." Folia Microbiologica 64, no. 5: 645-654.

Journal article
Published: 15 February 2019 in Applied and Environmental Microbiology
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Herein, we deciphered the most important biosynthetic traits of a prominent group of bioactive lipopeptides. We reveal evidence for initiation of biosynthesis by two alternative starter units hardwired directly in the same gene cluster, eventually resulting in the production of a remarkable range of lipopeptide variants. We identified several unusual tailoring genes potentially involved in modifying the fatty acid chain. Careful characterization of these biosynthetic gene clusters and their diverse products could provide important insight into lipopeptide biosynthesis in prokaryotes. Some of the variants identified exhibit cytotoxic and antifungal properties, and some are associated with a toxigenic biofilm-forming strain. The findings may prove valuable to researchers in the fields of natural product discovery and toxicology.

ACS Style

Jan Mareš; Jan Hájek; Petra Urajová; Andreja Kust; Jouni Jokela; Kumar Saurav; Tomáš Galica; Kateřina Čapková; Antti Mattila; Esa Haapaniemi; Perttu Permi; Ivar Mysterud; Olav M. Skulberg; Jan Karlsen; David P. Fewer; Kaarina Sivonen; Hanne Hjorth Tønnesen; Pavel Hrouzek. Alternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial Lipopeptides. Applied and Environmental Microbiology 2019, 85, 1 .

AMA Style

Jan Mareš, Jan Hájek, Petra Urajová, Andreja Kust, Jouni Jokela, Kumar Saurav, Tomáš Galica, Kateřina Čapková, Antti Mattila, Esa Haapaniemi, Perttu Permi, Ivar Mysterud, Olav M. Skulberg, Jan Karlsen, David P. Fewer, Kaarina Sivonen, Hanne Hjorth Tønnesen, Pavel Hrouzek. Alternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial Lipopeptides. Applied and Environmental Microbiology. 2019; 85 (4):1.

Chicago/Turabian Style

Jan Mareš; Jan Hájek; Petra Urajová; Andreja Kust; Jouni Jokela; Kumar Saurav; Tomáš Galica; Kateřina Čapková; Antti Mattila; Esa Haapaniemi; Perttu Permi; Ivar Mysterud; Olav M. Skulberg; Jan Karlsen; David P. Fewer; Kaarina Sivonen; Hanne Hjorth Tønnesen; Pavel Hrouzek. 2019. "Alternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial Lipopeptides." Applied and Environmental Microbiology 85, no. 4: 1.

Journal article
Published: 05 December 2018 in Food Chemistry
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Selenium (Se) is an indispensable microelement in our diet and health issues resulting from deficiencies are well documented. Se-containing food supplements are available on the market including Se-enriched Chlorella vulgaris (Se-Chlorella) which accumulates Se in the form of Se-amino acids (Se-AAs). Despite its popular uses, data about the bioaccessibility of Se-AAs from Se-Chlorella are completely missing. In the present study, gastrointestinal digestion times were optimized and the in vitro bioaccessibility of Se-AAs in Se-Chlorella, Se-yeast, a commercially available Se-enriched food supplement (Se-supplement) and Se rich foods (Se-foods) were compared. Higher bioaccessibility was found in Se-Chlorella (∼49%) as compared to Se-yeast (∼21%), Se-supplement (∼32%) and Se-foods. The methods used in production of Se-Chlorella biomass were also investigated. We found that disintegration increased bioaccessibility whereas the drying process had no effect. Similarly, temperature treatment by microwave oven also increased bioaccessibility whereas boiling water did not. Chemical compounds studied in this article Selenomethionine (PubChem CID: 15103); Selenocysteine (PubChem CID: 6326983); Methylselenocysteine (PubChem CID: 147004); Methanesulfonic acid (PubChem CID: 6395).

ACS Style

Dai Long Vu; Kumar Saurav; Mykola Mylenko; Karolína Ranglová; Jan Kuta; Daniela Ewe; Jiří Masojídek; Pavel Hrouzek. In vitro bioaccessibility of selenoamino acids from selenium (Se)-enriched Chlorella vulgaris biomass in comparison to selenized yeast; a Se-enriched food supplement; and Se-rich foods. Food Chemistry 2018, 279, 12 -19.

AMA Style

Dai Long Vu, Kumar Saurav, Mykola Mylenko, Karolína Ranglová, Jan Kuta, Daniela Ewe, Jiří Masojídek, Pavel Hrouzek. In vitro bioaccessibility of selenoamino acids from selenium (Se)-enriched Chlorella vulgaris biomass in comparison to selenized yeast; a Se-enriched food supplement; and Se-rich foods. Food Chemistry. 2018; 279 ():12-19.

Chicago/Turabian Style

Dai Long Vu; Kumar Saurav; Mykola Mylenko; Karolína Ranglová; Jan Kuta; Daniela Ewe; Jiří Masojídek; Pavel Hrouzek. 2018. "In vitro bioaccessibility of selenoamino acids from selenium (Se)-enriched Chlorella vulgaris biomass in comparison to selenized yeast; a Se-enriched food supplement; and Se-rich foods." Food Chemistry 279, no. : 12-19.

Journal article
Published: 16 October 2018 in Molecules
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Muscotoxins are cyanobacterial cyclic lipopeptides with potential applications in biomedicine and biotechnology. In this study, Desmonostoc muscorum CCALA125 strain extracts were enriched by polymeric resin treatment, and subjected to HPCCC affording three cyclic lipopeptides (1–3), which were further repurified by semi-preparative HPLC, affording 1, 2, and 3, with a purity of 86%, 92%, and 90%, respectively. The chemical identities of 2–3 were determined as muscotoxins A and B, respectively, by comparison with previously reported ESI-HRMS/MS data, whereas 1 was determined as a novel muscotoxin variant (muscotoxin C) using NMR and ESI-HRMS/MS data. Owing to the high yield (50 mg), compound 2 was broadly screened for its antimicrobial potential exhibiting a strong antifungal activity against Alternaria alternata, Monographella cucumerina, and Aspergillus fumigatus, with minimum inhibitory concentration (MIC) values of 0.58, 2.34, and 2.34 µg/mL; respectively, and weak antibacterial activity against Bacillus subtilis with a MIC value of 37.5 µg/mL. Compounds 1 and 3 were tested only against the plant pathogenic fungus Sclerotinia sclerotiorum due to their low yield, displaying a moderate antifungal activity. The developed chromatographic method proved to be an efficient tool for obtaining muscotoxins with potent antifungal properties.

ACS Style

José Cheel; Jan Hájek; Marek Kuzma; Kumar Saurav; Iva Smýkalová; Eliška Ondráčková; Petra Urajová; Dai Long Vu; Karine Faure; Jiří Kopecký; Pavel Hrouzek. Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A–C and Their Antimicrobial Activity. Molecules 2018, 23, 2653 .

AMA Style

José Cheel, Jan Hájek, Marek Kuzma, Kumar Saurav, Iva Smýkalová, Eliška Ondráčková, Petra Urajová, Dai Long Vu, Karine Faure, Jiří Kopecký, Pavel Hrouzek. Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A–C and Their Antimicrobial Activity. Molecules. 2018; 23 (10):2653.

Chicago/Turabian Style

José Cheel; Jan Hájek; Marek Kuzma; Kumar Saurav; Iva Smýkalová; Eliška Ondráčková; Petra Urajová; Dai Long Vu; Karine Faure; Jiří Kopecký; Pavel Hrouzek. 2018. "Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A–C and Their Antimicrobial Activity." Molecules 23, no. 10: 2653.