Accurate posture detection is the foundation for analyzing animal behavior, which can promote animal welfare. With the development of computer vision, such technology has been widely used in analyzing animal behavior without physical contact. However, computer vision technology for pig posture detection often suffers from problems of missed or false detection due to complex scenarios. To solve the problem, this study proposed a novel object detection model YOLOv5DA, which was based on YOLOv5s and designed for pig posture detection from 2D camera video. Firstly, we established the annotated dataset (7220 images) including the training set (5776 images), validation set (722 images), and test set (722 images). Secondly, an object detection model YOLOv5DA based on YOLOv5s was proposed to recognize pig postures (standing, prone lying, and side lying), which incorporated Mosaic9 data augmentation, deformable convolution, and adaptive spatial feature fusion. The comparative and ablation experiments were conducted to verify the model’s effectiveness and reliability. Finally, we used YOLOv5DA to detect the posture distribution of pigs. The results revealed that the standing posture was more frequent in the morning and afternoon and the side-lying posture was most common at noon. This observation demonstrated that the posture of pigs is influenced by temperature variations. The study demonstrated that YOLOv5DA could accurately identify three postures of standing, prone lying, and side lying with an average precision (AP) of 99.4%, 99.1%, and 99.1%, respectively. Compared with YOLOv5s, YOLOv5DA could effectively handle occlusion while increasing the mean precision (mAP) by 1.7%. Overall, our work provided a highly accurate, effective, low-cost, and non-contact strategy of posture detection in grouped pigs, which can be used to monitor pig behavior and assist in the early prevention of disease.

The development of multi-omics has increased the likelihood of further improving genomic prediction (GP) of complex traits. Gene expression data can directly reflect the genotype effect, and thus, they are widely used for GP. Generally, the gene expression data are integrated into multiple random effect models as independent data layers or used to replace genotype data for genomic prediction. In this study, we integrated pedigree, genotype, and gene expression data into the single-step method and investigated the effects of this integration on prediction accuracy. The integrated single-step method improved the genomic prediction accuracy of more than 90% of the 54 traits in the Duroc × Erhualian F₂ pig population dataset. On average, the prediction accuracy of the single-step method integrating gene expression data was 20.6% and 11.8% higher than that of the pedigree-based best linear unbiased prediction (ABLUP) and genome-based best linear unbiased prediction (GBLUP) when the weighting factor (w) was set as 0, and it was 5.3% higher than that of the single-step best linear unbiased prediction (ssBLUP) under different w values. Overall, the analyses confirmed that the integration of gene expression data into a single-step method could effectively improve genomic prediction accuracy. Our findings enrich the application of multi-omics data to genomic prediction and provide a valuable reference for integrating multi-omics data into the genomic prediction model.