Raffaella Isola

Dr. Raffaella Isola

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Biography

I am currently an Associate Professor of Histology in the Department of Biological Sciences at the University of Cagliari, Italy. My research began in pharmacology and neuroscience, focusing on nicotine and alcohol addiction, which included developing a mouse model of nicotine addiction during my two years at Ohio State University. I earned my PhD in Morphological Sciences at the University of Cagliari, studying mitochondrial potential in vivo with fluorescent probes. Subsequently, I shifted my focus to ultrastructural morphology, utilizing scanning and transmission electron microscopy to investigate the modifications of mitochondrial cristae in response to activity, as well as the morphological characteristics of salivary glands and other tissues under various conditions. I also had a brief research stint at the University of Heidelberg in Germany, where I worked on a project analyzing gamma oscillations in organotypic cultures of rat hippocampus, funded by DAAD. Later, I conducted research in Grenoble, France, studying mitochondrial bioenergetics in the hearts of AMPK knockout mice. Currently, my research centers on mitochondrial bioenergetics in cardiac and skeletal muscle mitochondria, as well as in murine myotubes. I employ Clark's oxygraph and various animal models. I am also interested in mitochondrial ultrastructural morphology with TEM and high-resolution scanning electron microscopy, employing osmium maceration for detailed visualization of mitochondrial membranes.

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