Changes inhspgene expression profiles in murrelChanna striatusexperimentally exposed to temperature stress (36°C) for 4, 15, and 30 days were investigated; fish collected from aquaculture ponds and maintained in laboratory at the pond temperature (25 ± 1°C) served as control.Channacollected from a hot spring runoff (36°C) was included in the study to examine thehspprofiles beyond 30 days of exposure. Gene expression analyses of a battery ofhspsin liver tissues were carried out by quantitative RT-PCR and protein expressions were analyzed by immunoblotting.hspscould be grouped into three clusters based on similarity in response to heat stress:hsp70, hsp78,andhsp60, whose transcript level continued to increase with duration of exposure;hsp90andhsp110that increased to a much higher level and then decreased;hsp27andhsp47that did not significantly vary as compared to control. The results suggest that Hsp70, Hsp78, and Hsp60 are involved in thermal acclimation and long term survival at high temperature. Fish living in the hot spring runoff appears to continuously expresshspsthat can be approximated by long term induction ofhspsin farmed fish if temperature of their environment is raised to 36°C.