Scanning ion conductance microscopy (SICM) enables the non-invasive three-dimensional imaging of live cells and other structures in physiological environments. However, when imaging complex samples, SICM faces challenges such as having a low temporal resolution during slow scanning and a reduced signal-to-noise ratio during fast scanning, making it difficult to simultaneously improve both temporal and spatial resolution. To address these issues, this paper proposes an algorithm for enhancing image resolution under high-speed scanning. Firstly, scanning images are preprocessed using a median filtering algorithm to remove the salt-and-pepper noise generated during high-speed scanning. Next, the Canny edge detection algorithm is employed to extract the edges of the image targets. To avoid blurring the edges, the new edge-directed interpolation (NEDI) algorithm is then used to fill the edges, while non-edge areas are filled using bilinear interpolation, thereby enhancing the image resolution. Finally, the peak signal-to-noise ratio (PSNR) and structural similarity index (SSIM) are used to analyze the imaging of articular chondrocytes. The results show that under a scanning speed of 480 nm/ms, the proposed algorithm improves the temporal resolution of imaging by 60% compared to traditional 2× resolution imaging, increases the peak signal-to-noise ratio of the scanning images by 7 dB, and achieves a structural similarity of 0.97. Therefore, the proposed algorithm effectively removes noise during high-speed scanning and improves the SICM scanning imaging resolution, thereby avoiding the reduction in temporal resolution when scanning larger resolution samples and effectively enhancing the performance of SICM scanning imaging.

With the advancement of artificial intelligence technology and computer hardware, the stereo matching algorithm has been widely researched and applied in the field of image processing. In scenarios such as robot navigation and autonomous driving, stereo matching algorithms are used to assist robots in acquiring depth information about the surrounding environment, thereby improving the robot’s ability for autonomous navigation during self-driving. In this paper, we address the issue of low matching accuracy of stereo matching algorithms in specular regions of images and propose a multi-attention-based stereo matching algorithm called MANet. The proposed algorithm embeds a multi-spectral attention module into the residual feature-extraction network of the PSMNet algorithm. It utilizes different 2D discrete cosine transforms to extract frequency-specific feature information, providing rich and effective features for cost computation in matching. The pyramid pooling module incorporates a coordinated attention mechanism, which not only maintains long-range dependencies with directional awareness but also captures more positional information during the pooling process, thereby enhancing the network’s representational capacity. The MANet algorithm was evaluated on three major benchmark datasets, namely, SceneFlow, KITTI2015, and KITTI2012, and compared with relevant algorithms. Experimental results demonstrated that the MANet algorithm achieved higher accuracy in predicting disparities and exhibited stronger robustness against specular reflections, enabling more accurate disparity prediction in specular regions.