Background: The importance of sheep breeding in the Mediterranean part of the eastern Adriatic has a long tradition since its arrival during the Neolithic migrations. Sheep production system is extensive and generally carried out in traditional systems without intensive systematic breeding programmes for high uniform trait production (carcass, wool and milk yield). Therefore, eight indigenous Croatian sheep breeds from eastern Adriatic treated here as metapopulation (EAS), are generally considered as multipurpose breeds (milk, meat and wool), not specialised for a particular type of production, but known for their robustness and resistance to certain environmental conditions. Our objective was to identify genomic regions and genes that exhibit patterns of positive selection signatures, decipher their biological and productive functionality, and provide a "genomic" characterization of EAS adaptation and determine its production type.Results: We identified positive selection signatures in EAS using several methods based on reduced local variation, linkage disequilibrium and site frequency spectrum (eROHi, iHS, nSL and CLR). Our analyses identified numerous genomic regions and genes (e.g., desmosomal cadherin and desmoglein gene families) associated with environmental adaptation and economically important traits. Most candidate genes were related to meat/production and health/immune response traits, while some of the candidate genes discovered were important for domestication and evolutionary processes (e.g.,HOXagene family andFSIP2). These results were also confirmed by GO and QTL enrichment analysis.Conclusions: Our results contribute to a better understanding of the unique adaptive genetic architecture of EAS and define its productive type, ultimately providing a new opportunity for future breeding programmes. At the same time, the numerous genes identified will improve our understanding of ruminant (sheep) robustness and resistance in the harsh and specific Mediterranean environment.

The majority of the nearly 400 existing local pig breeds are adapted to specific environments and human needs. The demand for large production quantities and the industrialized pig production have caused a rapid decline of many local pig breeds in recent decades. Black Slavonian pig and Turopolje pig, the latter highly threatened, are the two Croatian local indigenous breeds typically grown in extensive or semi-intensive systems. In order to guide a long-term breeding program to prevent the disappearance of these breeds, we analyzed their genetic diversity, inbreeding level and relationship with other local breeds across the world, as well as modern breeds and several wild populations, using high throughput genomic data obtained using the Illumina Infinium PorcineSNP60 v2 BeadChip. Multidimensional scaling analysis positioned Black Slavonian pigs close to the UK/North American breeds, while the Turopolje pig clustered within the Mediterranean breeds. Turopolje pig showed a very high inbreeding level (F_{ROH > 4 Mb} = 0.400 and F_{ROH > 8 Mb} = 0.332) that considerably exceeded the level of full-sib mating, while Black Slavonian pig showed much lower inbreeding (F_{ROH > 4 Mb} = 0.098 and F_{ROH > 8 Mb} = 0.074), indicating a planned mating strategy. In Croatian local breeds we identified several genome regions showing adaptive selection signals that were not present in commercial breeds. The results obtained in this study reflect the current genetic status and breeding management of the two Croatian indigenous local breeds. Given the small populations of both breeds, a controlled management activity has been implemented in Black Slavonian pigs since their commercial value has been recognized. In contrast, the extremely high inbreeding level observed in Turopolje pig argues for an urgent conservation plan with a long-term, diversity-oriented breeding program.

The aim of this paper was to highlight the most recent findings from research that utilized infrared thermography in diagnosing hoof disease and lameness in dairy cows. Scientific references were selected from databases based on their application of infrared thermography in detecting and preventing diseases in cattle. Studies have established that measuring view, leg position, and the presence of disease or different levels of lameness significantly affect the temperature values of cow’s feet. Additionally, the timing of measurements is also important, while factors such as lactation stage, animal activity, and ambient temperature substantially impact the infrared readings. The potential of using infrared thermography has been recognized. However, further research is needed to develop optimal combinations of different diagnostic methods to improve the accuracy and efficiency of detecting hoof disease and lameness.

The objective of this study was to compare the psysico-chemical composition and fatty acid (FFA) profile of traditional dry cured bacon Slavonska slanina, produced from the authentic Black Slavonian Pig (BP) and modern pigs (MP), using traditional homemade principles. The samples of Slavonska slanina produced from BP had a significantly (p < 0.05) higher fat content (78.32%) than the samples produced from MP (46.47%), while the samples produced from MP showed significantly higher (p < 0.05) protein and moisture content. The samples produced from BP also showed lower a_w and salt content but higher pH. Determination of fatty acid composition was performed at the end of the production process. The composition of fatty acids with respect to the groups of saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids was determined, as well as the ratios n-6/n-3 and PUFA/SFA. The results of fatty acid composition determination of Slavonska slanina produced from BP and MP show that oleic acid (C18: 1n9) is the most dominant fatty acid from the MUFA group (47.02 and 46.25%), the most common SFA acid was palmitic acid (C16: 0) (23.44 and 24.96%), and PUFA linoleic acid (C18: 2n-6c) (10.76 and 9.74%). The genotype had a significant (p < 0.05) effect on the SFA and USFA composition of Slavonska slanina. The ratios PUFA/SFA (0.34–0.28) and n-6/n-3 (31.84–27.34) for samples of Slavonska slanina produced from BP and MP are in concordance with previously published data for different dry cured meat products, and do not comply with the nutritionally recommended values of international health organizations (PUFA/SFA > 0.4 and n-6/n-3 < 4).

The Black Slavonian pig is an autochthonous pig breed in Croatia, which has recorded a continuous growth of the population followed by the higher number of breeders in recent years. The increase in population has removed the Black Slavonian breed from the category of endangered local breeds. The consequences of such a significant increase in population size in local breeds are often a high level of inbreeding, but also a high variability of the external traits of breeding individuals. Given that the main goal of the Breeding Program for Black Slavonian pigs is to preserve its phenotypic traits and breed-specific features, the paper presents the results of external traits analysis at 10 different points on the body, on a sample of 102 animals, aged between 10 and 24 months. Estimated mean values for wither’s height were 65 cm and 64 cm in boars and sows, respectively. Average body length was 128 cm for boars, and 126 cm for sows. The heart girth was 114 cm for both categories, while the height at the sacrum was 72 cm (boars), and 71 cm (sows). These results indicate very small or insignificant differences between male and female individuals, and refer that the body measurements of Black Slavonian pigs have not changed significantly in relation to its formation and development over time, as well as in relation to other local breeds from the neighbouring regions. With this in mind, selection work should be focused on control and preservation. Furthermore, the paper gives an overview of the population throughout history, as well as breeding practices.