Guiyang Shi

Dr. Guiyang Shi


Research Focus & Metrics

Academic Career Length

23Years (2004-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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27 May 2022 in Colloids and Surfaces B: Biointerfaces
Verified
Journal Article
Closed access
JLJiawei Lu
Jiawei Lu
MNMingfu Nie
Mingfu Nie
YLYouran Li
Youran Li
HZHuilin Zhu
Huilin Zhu
GSGuiyang Shi
Guiyang Shi

12 September 2022 in Journal of Advanced Research
Verified
Journal Article
Open access
HHHehe He
Hehe He
YLYouran Li
Youran Li
LZLiang Zhang
Liang Zhang
ZDZhongyang Ding
Zhongyang Ding
GSGuiyang Shi
Guiyang Shi
Nitrogen sources play an essential role in maintaining the physiological and biochemical activity of bacteria. Nitrogen metabolism, which is the core of microorganism metabolism, makes bacteria able to autonomously respond to different external nitrogen environments by exercising complex internal regulatory networks to help them stay in an ideal state. Although various studies have been put forth to better understand this regulation in Bacillus, and many valuable viewpoints have been obtained, these views need to be presented systematically and their possible applications need to be specified. The intention is to provide a deep and comprehensive understanding of nitrogen metabolism in Bacillus, an important industrial microorganism, and thereby apply this regulatory logic to synthetic biology to improve biosynthesis competitiveness. In addition, the potential researches in the future are also discussed. Understanding the meticulous regulation process of nitrogen metabolism in Bacillus not only could facilitate research on metabolic engineering but also could provide constructive insights and inspiration for studies of other microorganisms.

16 April 2014 in Biotechnology Letters
Verified
Journal Article
Closed access
PDF file
YSYan Shen
Yan Shen
LZLianzhen Zhao
Lianzhen Zhao
YLYouran Li
Youran Li
LZLiang Zhang
Liang Zhang
GSGuiyang Shi
Guiyang Shi

8 March 2021 in Journal of Agricultural and Food Chemistry
Verified
Journal Article
Closed access
JWJunhua Wang
Junhua Wang
WJWei Jiang
Wei Jiang
CLChaojuan Liang
Chaojuan Liang
LZLinghuan Zhu
Linghuan Zhu
YLYouran Li
Youran Li
GSGuiyang Shi
Guiyang Shi

1 February 2019 in Bioresource Technology
Verified
Journal Article
Closed access
YLYouran Li
Youran Li
HWHanrong Wang
Hanrong Wang
JLJiawei Lu
Jiawei Lu
ACAlex Chu
Alex Chu
LZLiang Zhang
Liang Zhang
GSGuiyang Shi
Guiyang Shi

1 January 2017 in Journal of Industrial Microbiology and Biotechnology
Verified
Journal Article
Open access
PDF file
HLHua Li
Hua Li
BWBao Shi Wang
Bao Shi Wang
YLYou Ran Li
You Ran Li
LZLiang Zhang
Liang Zhang
ZDZhong Yang Ding
Zhong Yang Ding
GSGuiyang Shi
Guiyang Shi
In this study, we constructed an l-methionine-producing recombinant strain from wild-type Escherichia coli W3110 by metabolic engineering. To enhance the carbon flux to methionine and derepression met regulon, thrBC, lysA, and metJ were deleted in turn. Methionine biosynthesis obstacles were overcome by overexpression of metAFbr (Fbr, Feedback resistance), metB, and malY under control of promoter pN25. Recombinant strain growth and methionine production were further improved by attenuation of metK gene expression through replacing native promoter by metK84p. Blocking the threonine pathway by deletion of thrBC or thrC was compared. Deletion of thrC showed faster growth rate and higher methionine production. Finally, metE, metF, and metH were overexpressed to enhance methylation efficiency. Compared with the original strain E. coli W3110, the finally obtained Me05 (pETMAFbr-B-Y/pKKmetH) improved methionine production from 0 to 0.65 and 5.62 g/L in a flask and a 15-L fermenter, respectively.