Balanced fertilizer management promotes plant growth, enhances produce quality, minimizes inputs, and reduces negative environmental impacts. Wax gourd (Benincasa hispida) is an important vegetable crop species in China and in South Asia. Two crop nutrition options, NPK and the natural mineral polyhalite, were tested, separately and combined, with the aim of enhancing wax gourd yield and quality and simultaneously to increase nutrient use efficiency and reducing inputs. The experiments tested the optimization of NPK by reducing the proportion of phosphorus (P), and the effect of enriching the soil with essential macronutrients by the use of the supplementary mineral fertilizer polyhalite containing magnesium (Mg), calcium (Ca) and sulfur (S). Two experiments were carried out in Foshan County, Guangdong, China, in 2018 and 2019. Experiments included four treatments: (1) Conventional NPK (15:15:15); (2) Optimized NPK (16:8:18); (3) Conventional NPK + polyhalite; (4) Optimized NPK + polyhalite. Fertilizers were applied prior to planting. While optimized NPK alone had no effects on fruit yield and quality, supplementary polyhalite resulted in a 10–17% increase in yield and significantly improved produce quality due to increased nutrient uptake from polyhalite, resulting in better foliar biomass. We conclude that the combined crop nutrition options improved yield and quality, enhanced nutrient use efficiency, and reduced risks of nutrient pollution. Inclusion of polyhalite in balanced fertilization practices as a supplementary source of secondary macronutrients seems promising. Nevertheless, plenty of space remains open for further adjustments of NPK application management, focusing on reduced rates, optimized ratio, and accurate timing of application for each nutrient.

The three-amino-loop-extension (TALE) family involves key transcription factors vital for maintaining different aspects of growth including leaf, flower, and fruit development and responses to stressful stimulus. Thus far, a deep understanding of the TALE gene family in wax gourd subjected to low calcium and magnesium stress has been missing. Here, we isolated 24 BhTALE genes from a wax gourd genome database. Comprehensive bioinformatic analysis, including evolutionary tree, gene structures, conserved motifs, and chemical properties, provide structural and functional insights into the BhTALE gene family. Gene ontology (GO) analysis of TALE genes unveils their involvement in growth and stress responses. Promoter analysis indicates that hormones and stresses can influence the expression of BhTALE genes. Tissue-specific expression under low calcium and magnesium stress showed that BhTALE genes were more active in the leaves and roots. Notably, BhTALE7, BhTALE10, and BhTALE14 were expressed differentially in leaves under low calcium and magnesium applications. Similarly, the induced expression pattern of BhTALE4 was recorded in the roots under low calcium and magnesium applications. Our findings underscore the pivotal role of the BhTALE gene family in dealing with low calcium and magnesium stress in the wax gourd.