In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K₂HPO₄, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated. Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K₂HPO₄, 20% [Chol]Cl, 10.0 mg mL⁻¹ crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively. Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations. Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS. Therefore, the method of [Chol]Cl/K₂HPO₄ ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.

In this study, simultaneous separation and recovery of Cs(I) and Sr(II) from nitric acid solution was investigated using a silica-based hybrid adsorbent. The adsorbent was prepared by successive impregnation and fixing of two supramolecular recognition agents namely, 1,3-[(2,4-diethylheptylethoxy)oxy]-2,4-crown-6-Calix[4]-arene(Calix[4]arene-R14) and 4',4' (5")-di(tert-butylcyclohexano)-18-crown-6, onto a silica-based polymer support(SiO₂-P). Uptake properties, characterization, equilibrium kinetics, and dynamic adsorption properties of Cs(I) and Sr(II) were then assessed. Distribution coefficients (K_d) higher than 10² cm³/g for Cs(I) and Sr(II) were obtained using 3 M HNO₃ at 298 K, and the K_d values decreased with increasing temperature. Adsorption kinetics and equilibrium studies fitted well with pseudo-second-order model and Redlich–Peterson isotherm model, respectively. The constant total organic carbon values in the aqueous phase were obtained after adding 10⁻³ ∼ 4 M HNO₃. Results of the dynamic adsorption/elution test indicated that Cs(I) and Sr(II) were efficiently and simultaneously captured and eluted even under a flow rate of 1.5 cm³/min.