Drought is a significant environmental limiting factor that restricts the growth of Quercus wutaishanica Mayr. The MYB transcription factor plays a wide role in controlling the growth of plants. In this study, the QwMYB108 gene was cloned and the bioinformatics was analyzed, and we examined how QwMYB108 responded to various gradient drought stresses. The results demonstrated that QwMYB108 encoded 275 amino acids using an 828 bp open reading frame. Subcellular localization indicated that the gene was located in the nucleus. Phylogenetic analysis showed that QwMYB108 was close to Q. robur, and that the highest level of expression was found in leaves, which was significantly different from other tissues. The expression of QwMYB108 increased as the stress degree rose when drought stress was present, and there was a significant difference between severe drought stress and other gradient stress. In this study, the function of QwMYB108 in drought stress response was investigated, and the drought response function gene of Q. wutaishanica was further explored to provide a theoretical basis.

Actinidia arguta is a Chinese native fruiting vine and has been cultivated in several countries for its commercial advantages (Williams et al. 2003). During the 2017 and 2018 growing seasons, leaf ...

Chloroplast glycerol-3-phosphate acyltransferase (GPAT), the primary enzyme influencing phosphatidylglycerol unsaturation in thylakoid membranes, plays a crucial role in regulating plant cold tolerance. Ammopiptanthus nanus exhibits significant cold stress tolerance due to its unique growth environment. The characterization of the GPAT gene in A. nanus remains largely unexplored. This study isolated and analyzed the sequence structure of the AnGPAT gene and its promoter in A. nanus. Phylogenetic analysis indicated that AnGPAT is closely related to GPATs from Glycine max, Lupinus albus, and Glycine soja, all members of the Fabaceae family. The subcellular localization indicated that AnGPAT is localized in the chloroplast membrane. AnGPAT transcription levels increased in response to cold stress and ABA treatment. Overexpression of AnGPAT in Arabidopsis thaliana enhanced seed germination and transgenic seedling growth under cold stress conditions. In transgenic plants under cold stress, proline content increased, while malondialdehyde content and relative electrolyte leakage decreased. The content of GPAT enzyme and unsaturated fatty acids in transgenic plants were significantly higher than in WT plants under cold stress. In addition, the promoter of AnGPAT includes MYB/MYC elements responsive to cold and ABRE elements responsive to ABA, which trigger GUS protein expression under cold stress and ABA treatment. The findings demonstrated that AnGPAT improved cold tolerance in transgenic plants. This study provides evidence for further understanding the regulation mechanism related to ABA and cold stress in A. nanus.