The global acceptance of the SARS-CoV-2 airborne transmission led to prevention measures based on quality control and air renewal. Among them, carbon dioxide (CO₂) measurement has positioned itself as a cost-efficiency, reliable, and straightforward method to assess indoor air renewal indirectly. Through the control of CO₂, it is possible to implement and validate the effectiveness of prevention measures to reduce the risk of contagion of respiratory diseases by aerosols. Thanks to the method scalability, CO₂ measurement has become the gold standard for diagnosing air quality in shared spaces. Even though collective transport is considered one of the environments with the highest rate of COVID-19 propagation, little research has been done where the air inside vehicles is analyzed. This work explores the generation and accumulation of metabolic CO₂ in a tramway (Zaragoza, Spain) operation. Importantly, we propose to use the indicator ppm/person as a basis for comparing environments under different conditions. Our study concludes with an experimental evaluation of the benefit of modifying some parameters of the Heating–Ventilation–Air conditioning (HVAC) system. The study of the particle retention efficiency of the implemented filters shows a poor air cleaning performance that, at present, can be counteracted by opening windows. Seeking a post-pandemic scenario, it will be crucial to seek strategies to improve air quality in public transport to prevent the transmission of infectious diseases.

The spread dynamics of the SARS-CoV-2 virus have not yet been fully understood after two years of the pandemic. The virus’s global spread represented a unique scenario for advancing infectious disease research. Consequently, mechanistic epidemiological theories were quickly dismissed, and more attention was paid to other approaches that considered heterogeneity in the spread. One of the most critical advances in aerial pathogens transmission was the global acceptance of the airborne model, where the airway is presented as the epicenter of the spread of the disease. Although the aerodynamics and persistence of the SARS-CoV-2 virus in the air have been extensively studied, the actual probability of contagion is still unknown. In this work, the individual heterogeneity in the transmission of 22 patients infected with COVID-19 was analyzed by close contact (cough samples) and air (environmental samples). Viral RNA was detected in 2/19 cough samples from patient subgroups, with a mean Ct (Cycle Threshold in Quantitative Polymerase Chain Reaction analysis) of 25.7 ± 7.0. Nevertheless, viral RNA was only detected in air samples from 1/8 patients, with an average Ct of 25.0 ± 4.0. Viral load in cough samples ranged from 7.3 × 10⁵ to 8.7 × 10⁸ copies/mL among patients, while concentrations between 1.1–4.8 copies/m³ were found in air, consistent with other reports in the literature. In patients undergoing follow-up, no viral load was found (neither in coughs nor in the air) after the third day of symptoms, which could help define quarantine periods in infected individuals. In addition, it was found that the patient’s Ct should not be considered an indicator of infectiousness, since it could not be correlated with the viral load disseminated. The results of this work are in line with proposed hypotheses of superspreaders, which can attribute part of the heterogeneity of the spread to the oversized emission of a small percentage of infected people.

The measurement of NO_x emissions in vehicles has so far been exclusively carried out during the type-approval process. For this purpose, high-precision gas measurement laboratory equipment and Portable Emission Measurement Systems (PEMS) are used. Both types of equipment are costly in terms of price, maintenance, complexity, and time of use (calibration and maintenance requirements). Currently, NO_x emissions measurements in Periodic Technical Inspections (PTIs) are being considered, but PEMS or laboratory equipment is unsuitable for this function, and PTI-grade equipment has to be used. Although CO and O₂ are currently being reliably measured with this equipment, there is not enough information about its accuracy for NO_x measurements. Accordingly, in this paper, simultaneous measures have been performed over the same engine in a test cell, with a laboratory and a PTI gas analyser to assess the accuracy of the latter. When performing the test with the most similar conditions found in PTI, our results show that the PTI gas analyser shows an average deviation of 2.6 ppm and 9% rel. with respect to high-precision laboratory equipment for concentrations below 700 ppm NO_x, which can be considered acceptable for periodic technical inspections.

NOx emissions in vehicles are currently only controlled through the homologation process. There is a lack of knowledge to assess and control real NOx emissions of vehicles reliably. Even if vehicles in EU-27 are subject to Periodical Technical Inspection (PTI), NOx are not among the pollutants currently being controlled. For PTIs, tests need to be simple, quick, inexpensive, representative, and accurate. Ideally, tests need to be carried out under static conditions, without the need for a power bench or complex equipment. In this paper, a new approach for measuring NOx in PTI is proposed. The method has been developed and validated at a PTI Spanish station to ensure feasibility and repeatability. This method is based on the relationship between the “% engine load” value and exhaust NOx concentration at idle engine speed. Starting from the state of minimum possible power demand in a vehicle (idling and without any consumption), a load state with an average 98% increase in engine power demand is generated by connecting elements of the vehicle’s equipment. The relationship between power demand (through the “% engine load” value) and NOx concentration is then analyzed. The quality and representativity of this relationship have been checked with a p-value lower than 0.01. The method has been compared with a different NOx measurement technique, based on the simulation on a test bench and the ASM 2050 cycle, showing better performance in terms of repeatability and representativeness. The “% engine load” dispersion with the new approach is 7%, which ensures the reliability and repeatability of the method. The results show that the proposed method could be a valuable tool in PTI to detect high NOx emitting vehicles and to obtain information from the diesel vehicles fleet.

The measurement of carbon dioxide (CO₂) has emerged as a cost-effective and straightforward technique for indirectly managing indoor air quality, aiding in the reduction of the potentially pathogen-laden aerosol concentrations to which we are exposed. Unfortunately, inadequate practices often limit the interpretation of CO₂ levels and neglect methodologies that ensure proper air renewal. This study presents a novel methodology for measuring and controlling indoor CO₂ levels in shared spaces, comprising four stages: analysis, diagnosis, correction protocols, and monitoring/control/surveillance (MCS). This methodology underwent validation in practical settings, including a cultural center (representing spaces with uniform activities) and 40 commercial spaces (with diverse activities) in Zaragoza, Spain. The results indicate the feasibility of swiftly implementing measures to enhance shared air renewal, with the immediate opening of doors and windows being the most direct solution. The proposed methodology is practical and has the potential to mitigate the risk of the aerosol transmission of respiratory diseases. Consequently, we anticipate that this work will contribute to establishing methodological foundations for CO₂ measurement as a valuable, standardized, and reliable tool.