The Manila clam (Ruditapes philippinarum) is one of the most commercially important bivalves along the coast of China. With the continuous expansion of clam farming scale, it may lead to some serious problems, including loss of genetic variation, inbreeding depression, and reduced effective population size (N_e). In the present study, eleven microsatellite markers were used to investigate the genetic diversity and differentiation among 13 clam populations along the coast of China. As a result, 150 alleles were detected according to the genotyping results of eleven microsatellite loci. The observed heterozygosity (H_o) was estimated to be ranging from 0.437 to 0.678, while the expected heterozygosity (H_e) was calculated to be varying from 0.587 to 0.700. F_st values between populations ranged from 0.0046-0.1983. In particular, the Laizhou population had the highest genetic variability, which was significantly different from the others (all F_st values > 0.1). For all the clam populations, there was no significant linear regression between genetic and geographic distance, indicating that these populations do not follow a pattern of isolation by distance (IBD). Genetic structure was estimated according to NJ, principal coordinates (PCoA), and structure-based clustering. Estimates of effective population size range from dozens to thousands among different populations, based on linkage-disequilibrium and molecular coancestry methods. The results reveal the genetic diversity of clams and verify the hypothesis that clam population differentiation may be influenced by the mode of southern breeding and northern culture, providing guiding information for natural resource conservation and genetic breeding of clams.

Architectural vertical green walls can mitigate the urban heat island effect, provide shade and cooling, reduce energy consumption, improve a microclimate, and increase indoor comfort. However, an excessive pursuit of high coverage may diminish the benefit ratio and adversely affect ventilation and lighting. Field measurements were conducted in the hot and humid Guangzhou area to investigate the thermal benefits of external vertical green walls with varying green coverage and diverse layouts, encompassing effects such as shading, insulation, cooling, and humidification. Analyses were conducted using ENVI-met, orthogonal experiments, and SPSS to quantify the moderating effects of planted green coverage (PGC), leaf area density (LAD), and air interstitial layers on the environmental thermal benefits. The results indicated that the cooling and humidifying effects of 100% PGC and 75% PGC were comparable and superior to those of 50% PGC, yet 75% PGC outperformed 100% PGC in terms of indoor humidification. Among the layout modes, the horizontal layout was the most effective for cooling and humidification, followed by the point layout, with the vertical layout being the least effective. A global sensitivity analysis revealed that PGC had the greatest impact on wall cooling and outdoor humidification, LAD significantly influenced humidification, the width of air interstitial layers had a minor impact, and the two architectural vertical greening design ratios of 75% PGC × 4.60 LAD and 75% PGC × 2.70 LAD were particularly effective for cooling and humidification. Incorporating horizontal or point-like layouts can enhance façade design diversity while preserving the desired environmental thermal benefits, thereby contributing to the overall aesthetics of a building.