Chrysomycin A is one of the most promising therapeutic candidates for treating infections caused by multidrug-resistant Gram-positive bacteria. By hybridizing next-step generation (Illumina) and third-generation (PacBio) sequencing technologies, a high-quality chromosome-level genome together with a plasmid was firstly assembled for chrysomycin A-producing marine strain 891. Phylogenetic analysis of the 16S rRNA gene and genome sequences revealed that this strain unambiguously belonged to the genus Streptomyces, and its genomic features and functional genes were comprehensively analyzed and annotated. AntiSMASH analysis of this strain unveiled one key biosynthetic gene cluster, T2PKS, responsible for the biosynthesis of chrysomycin, the biosynthesis pathway of which was putatively proposed. These findings definitely shed light on further investigation for construction of a robust industrial strain with high-yield chrysomycin A production using genetic engineering techniques and combinatorial biology approaches.

Climatic niche breadth is defined as the range of climatic conditions (e.g., temperature and precipitation) under which a species occurs. However, the relationship between niche breadth variation and climatic factors remains poorly studied, and existing results require more general testing. We studied spatial patterns of variation in climatic niche breadths in lizards of the family Agamidae and compared patterns within and across regions to see if they parallel or differ from each other using geo-referenced occurrence records, climatic data and phylogenetic comparative methods. We found that (1) species in warmer environments have narrower temperature niche breadths; (2) precipitation niche breadths are positively correlated with precipitation niche position, and also with temperature niche breadths; and (3) most of the variation in temperature niche breadths is explained by within-locality variation in climatic conditions, whereas most of the variation in precipitation niche breadths is explained by among-locality variation. The patterns of climatic niche breadth in agamids are consistent across regional and global scales, similar to those in other amphibians and reptiles. This suggests that this is a widespread phenomenon among ectothermic vertebrates.