The optimum conditions for growth, sporulation, and crystal-formation in four isolated crystal-forming strains of Bacillus laterosporus were determined. It was shown that culture broth and pellets of bacterial culture liquid possess larvicidal activity against larvae of mosquitoes A. stephensi and A. aegypti. The protein nature of crystal was shown. Crystals are monocomponent containing a protein with MM of 68 or 130 kDa. Purified protein crystals demonstrated larvicidal activity. Specific larvicidal activity of crystals of various strains essentially differed. High larvicidal activity of B. laterosporus strains allows for them to be recommended as producers of antimosquito biological preparations.

Bovine coronaviruses (BCoVs), which cause gastrointestinal and respiratory diseases in cattle, and are genetically related to the human coronavirus HCoV-OC43, which is responsible for up to 10% of common colds, attract increased attention. We applied the method of photodynamic inactivation with cationic photosensitizers (PSs) to reduce the titers of BCoV and studied the morphological structure of viral particles under various modes of photodynamic exposure. The samples of virus containing liquid with an initial virus titer of 5 Log₁₀ TCID50/mL were incubated with methylene blue (MB) or octakis(cholinyl)zinc phthalocyanine (Zn-PcChol₈₊) at concentrations of 1–5 μM for 10 min in the dark at room temperature. After incubation, samples were irradiated with LED (emission with maximum at 663 nm for MB or at 686 nm for Zn-PcChol₈₊) with light doses of 1.5 or 4 J/cm². Next, the irradiation titrated virus containing liquid was studied using negative staining transmission electron microscopy. MB and Zn-PcChol₈₊ at concentrations of 1–5 μM, in combination with red light from LED sources in the low doses of 1.5–4.0 J/cm², led to a decrease in BCoV titers by at least four orders of magnitude from the initial titer 5 Log₁₀ TCID50/mL. Morphological changes in photodamaged BCoVs with increasing PS concentrations were loss of spikes, change in shape, decreased size of virus particles, destruction of the envelope, and complete disintegration of viruses. BCoV has been found to be sensitive to MB, which is the well-known approved drug, even in the absence of light.

The article analyzes and summarizes the literary and own results of studies of the properties of Brevibacillus laterosporus (Bl) as an agent of biocontrol of insects, microorganisms, and other invertebrates. The review provides data on the morphology and characteristics of the biological properties of Bl. New crystal-bearing strains were reported. The structural features of spores and Bl crystals identified by electron microscopy are discussed. Data on crystal formation in different bacilli are analyzed. Bl crystals have mosquitocidal activity. Data on the antimicrobial properties of Bl, including the activity of Bl against drug-resistant bacteria, are presented. The antimicrobial, fungicidal, and cyanolytic activities of Bl strains make it possible to use them as producers of insecticides, antibiotics, and bacteriocins and as environmentally friendly bacterial agents of biocontrol.

Bacillus cereus sensu lato comprises genetically, morphologically, and physiologically similar gram-positive spore-forming bacterial species with high pathogenic potential, such as B. anthracis, B. cereus, and B. thuringiensis. Toxin-producing strains of B. cereus s.l. pose a major threat to human health. The high degree of similarity between these species makes it very difficult to identify them and to take adequate measures to treat the diseases they cause. Previously, we characterized the clinical isolate CCGC 19/16 belonging to B. cereus s.l. that exhibited features of both B. cereus and B. cytotoxicus. In the present work, CCGC 19/16 was identified as B. cytotoxicus using multilocus sequence typing (MLST) and mass spectrometric analysis. It was also shown that, unlike other representatives of the B. cytotoxicus species, strain CCGC 19/16 is not thermotolerant. Unlike B. cereus, strain CCGC 19/16 is sensitive to most antibiotics and shows increased motility. Like B. cereus strain CCGC 19/16 forms β-hemolysis zones in blood agar. In addition, it has been shown that prolonged storage of samples prior to analysis can lead to misidentification of the isolate. Our results indicate that “rapid methods” of analysis using single genes have insufficient resolving power in the identification of B. cereus s.l. species. The combination of MLST analysis with MALDI-TOF MS provides sufficient resolution.

The article analyzes and summarizes the literary and own results of studies of the properties of Brevibacillus laterosporus (Bl) as an agent of biocontrol of insects, microorganisms, invertebrates. The review provides data on the morphology and characteristics of the biological properties of Bl. New crystal-bearing strains were reported. The structural features of spores and Bl crystals identified by electron microscopy are discussed. Data on crystal formation in different bacilli are analyzed. Bl crystals have mosquitocidal activity. Data on the antimicrobial properties of Bl, including the activity of Bl against drug-resistant bacteria, are presented. The antimicrobial, fungicidal and cyanolytic activities of Bl strains make it possible to use them as producers of insecticides, antibiotics, bacteriocins and as environmentally friendly bacterial agents of biocontrol.