Optimal potassium (K) fertilizer application in potato cropping systems can effectively increase food production and mitigate soil microbial ecosystem stress. The dynamics and sustainability of potato yield, the dynamics of potato commodity rates (CRs), and microbial community structure were explored under four different K application rates (kg K ha⁻¹ year⁻¹): 0 (control), 75 (low K), 150 (medium K), and 225 (high K). Compared with the low-K application, the medium-K and high-K applications increased potato yields by 8.08% and 11.66%, respectively. The mean CR of potato tubers during 4 years was significantly greater under the medium-K treatment than under the low-K and high-K treatments. Both medium-K and high-K applications significantly enhanced the sustainable yield index (SYI) relative to the Low-K application by 7.93% and 9.34%, respectively. Compared with the zero-K, low-K, and high-K treatments, the medium-K treatment improved the total phospholipid fatty acid (PLFA) contents by 11.91%, 16.84%, and 11.66%, respectively. Moreover, the medium-K application increased the bacterial PFLA, actinomycete PFLA, gram-positive (G⁺) bacterial PFLA, and gram-negative (G⁻) bacterial PFLA contents in the soil. Overall, application of 150 kg ha⁻¹ year⁻¹ K fertilizer represents a promising fertilization strategy in potato cropping systems in Southwest China.