Significance: Asparagine-linked glycosylation is one of the most ubiquitous posttranslational protein modifications in all kingdoms of life. A membrane enzyme, oligosaccharyltransferase (OST), catalyzes the transfer of sugar chains onto the asparagine residues of the Asn-X-Ser/Thr sequon in acceptor proteins. We determined the structures of an archaeal OST in two crystal forms. The comparison with the previous eubacterial OST structure revealed the structural conservation of the catalytic core and the membrane-spanning region. The archaeal and eubacterial OST structures probably correspond to different steps in the OST reaction cycle. The conversion between the structured and unstructured states of a characteristic loop in the membrane-spanning region is the structural basis for the dynamic recognition of the Asn residue in the sequon.

Oligosaccharyltransferase (OST) catalyzes the transfer of an oligosaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains. In the bacterium Campylobacter jejuni, a single-subunit membrane protein, PglB, catalyzes N-glycosylation. We report the 2.8 Å resolution crystal structure of the C-terminal globular domain of PglB and its comparison with the previously determined structure from the archaeon Pyrococcus AglB. The two distantly related oligosaccharyltransferases share unexpected structural similarity beyond that expected from the sequence comparison. The common architecture of the putative catalytic sites revealed a new catalytic motif in PglB. Site-directed mutagenesis analyses confirmed the contribution of this motif to the catalytic function. Bacterial PglB and archaeal AglB constitute a protein family of the catalytic subunit of OST along with STT3 from eukaryotes. A structure-aided multiple sequence alignment of the STT3/PglB/AglB protein family revealed three types of OST catalytic centers. This novel classification will provide a useful framework for understanding the enzymatic properties of the OST enzymes from Eukarya, Archaea, and Bacteria.

Lannea edulis (Sond.) Engl. commonly known as wild grape is used traditionally for the treatment of diabetes. It is only found in Eastern and Southern Africa. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of L. edulis in alloxan induced diabetic rats were carried out. We report herein the findings of this research work. Lannea edulis crude aqueous extracts were obtained by hot infusion and evaporation method. Phytochemical screening was carried out and subsequently toxicity studies of the aqueous extracts were performed to establish the Lethal Dose 50 (LD₅₀) in albino rats. Alloxan monohydrate was used to induce diabetes in the rats. Lannea edulis positive control group doses of 100, 300, and 500 mg/kg were administered to 3 groups for 14 days. The positive control group was administered 5 mg/kg of glibenclamide. The negative and normal control groups were administered distilled water. To determine fasting blood glucose, blood was drawn on days 0, 1, 3, 5, 7, and 14 while it was drawn on days 0 and 14 for the determination of lipids. Phytochemical screening revealed the presence of flavonoids, saponins, tannins, cardiac glycosides, alkaloids and steroids. L. edulis diabetic positive control groups showed significant (P < 0.05) dose dependent reductions in fasting blood glucose levels. When day 0 mean blood glucose levels were compared to day 3 mean blood glucose levels of their respective groups, the 300 mg/kg L. edulis group showed a 23.3% drop and the 500 mg/kg L. edulis group showed a 52.6% drop. The 100 mg/kg L. edulis diabetic positive control group showed a 25.1% drop by day 5, the day on which it showed statistical significance (P < 0.05) compared to the diabetic control. In addition, administration of aqueous extracts of L. edulis to diabetic rats for 14 days significantly decreased (P < 0.05) the levels of serum total cholesterol, triglycerides, Low Density Lipoprotein (LDL) and Very Low Density Lipoprotein (VLDL) whilst increasing the levels of High Density Lipoprotein (HDL), when compared to the diabetic control group. It was concluded that L. edulis showed significant and dose dependent antihyperglycemic and antihyperlipidemic effects thus confirming its traditional use.

In this paper, we report extraction of cashew nut shell liquid (CNSL) from cashew nut shell waste (CNSW) and further use of residues for generation of activated carbon for removal of heavy metals and methylene blue (MB). Solvent extraction yielded 24.6 ± 0.4%, 38.2 ± 0.4% and 40.1 ± 0.9% for petroleum ether, hexane and ethanol respectively. Phytochemical screening showed presence of alkaloids, carbohydrates, saponins, phenols, tannins, flavonoids, amino acids, terpenoids, proteins, steroids, glycosides and carboxylic acids. The CNSL had a pH of 3.2, viscosity (104.6 ± 1.8 mPa s), moisture (6.5%), ash (1.6 ± 0.1%), refractive index (1.52 ± 0.001), specific density (0.9561 ± 0.0002 g/cm³), acid value (118.7 ± 9.2 mg KOH/g), free fatty acid value (60.1 ± 4.7%), saponification number (138.1 ± 3.2 mg KOH/g) and iodine value (188.1 ± 2.3 mgI 2/100 g). The average percentage removal of Cu (II), Pb (II), Cd (II) and Zn (II) was 99.4 ± 0.5, 95.4 ± 1.5, 99.5 ± 0.1, 98.4 ± 0.1%, and removal efficiency of MB at 50, 150, 250 and 350 mg/L was 99.63, 97.66, 96.48 and 94.81%, respectively. Equilibrium data were best described by the Freundlich isotherm model. The maximum monolayer adsorption capacity was 12.1 mg/g. The adsorption kinetics conformed to pseudo-second-order model. ∆G° was negative and a ∆H° of + 22.76 kJ/mol indicated that adsorption was endothermic. The ΔS° (+ 0.086 kJ/mol/K) showed that there was spontaneous interaction of the solution and adsorbate. These results show that CNSW is a potential bioresource for CNSL production for use in the paints, varnishes, surface coatings, agrochemicals and ethnomedicine industries. Residual shells can be exploited as fuels or converted to activated carbon for use as low-cost filters in water purification.