Insect pests are a major element influencing agricultural production. According to the Food and Agriculture Organization (FAO), an estimated 20–40% of pest damage occurs each year, which reduces global production and becomes a major challenge to crop production. These insect pests cause sooty mold disease by sucking the sap from the crop’s organs, especially leaves, fruits, stems, and roots. To control these pests, pesticides are frequently used because they are fast-acting and scalable. Due to environmental pollution and health awareness, less use of pesticides is recommended. One of the salient approaches could be to reduce the wide use of pesticides by spraying on demand. To perform spot spraying, the location of the pest must first be determined. Therefore, the growing population and increasing food demand emphasize the development of novel methods and systems for agricultural production to address environmental concerns and ensure efficiency and sustainability. To accurately identify these insect pests at an early stage, insect pest detection and classification have recently become in high demand. Thus, this study aims to develop an object recognition system for the detection of crops damaging insect pests and their classification. The current work proposes an automatic system in the form of a smartphone IP- camera to detect insect pests from digital images/videos to reduce farmers’ reliance on pesticides. The proposed approach is based on YOLO object detection architectures including YOLOv5 (n, s, m, l, and x), YOLOv3, YOLO-Lite, and YOLOR. For this purpose, we collected 7046 images in the wild under different illumination and background conditions to train the underlying object detection approaches. We trained and test the object recognition system with different parameters from scratch. The eight models are compared and analyzed. The experimental results show that the average precision (AP@0.5) of the eight models including YOLO-Lite, YOLOv3, YOLOR, and YOLOv5 with five different scales (n, s, m, l, and x) reach 51.7%, 97.6%, 96.80%, 83.85%, 94.61%, 97.18%, 97.04%, and 98.3% respectively. The larger the model, the higher the average accuracy of the detection validation results. We observed that the YOLOv5x model is fully functional and can correctly identify the twenty-three species of insect pests at 40.5 milliseconds (ms). The developed model YOLOv5x performs the state-of-the-art model with an average precision value of (mAP@0.5) 98.3%, (mAP@0.5:0.95) value of 79.8%, precision of 94.5% and a recall of 97.8%, and F1-score with 96% on our IP-23 dataset. The results show that the system works efficiently and was able to correctly detect and identify insect pests, which can be employed for realistic application while farming.

Background: Over 200 published studies of more than 30 plant species have reported a role for miRNAs in regulating responses to abiotic stresses. However, data from these individual reports has not been collected into a single database. The lack of a curated database of stress-related miRNAs limits research in this field, and thus a cohesive database system should necessarily be constructed for data deposit and further application. Description: PASmiR, a literature-curated and web-accessible database, was developed to provide detailed, searchable descriptions of miRNA molecular regulation in different plant abiotic stresses. PASmiR currently includes data from ~200 published studies, representing 1038 regulatory relationships between 682 miRNAs and 35 abiotic stresses in 33 plant species. PASmiR’s interface allows users to retrieve miRNA-stress regulatory entries by keyword search using plant species, abiotic stress, and miRNA identifier. Each entry upon keyword query contains detailed regulation information for a specific miRNA, including species name, miRNA identifier, stress name, miRNA expression pattern, detection method for miRNA expression, a reference literature, and target gene(s) of the miRNA extracted from the corresponding reference or miRBase. Users can also contribute novel regulatory entries by using a web-based submission page. The PASmiR database is freely accessible from the two URLs of http://hi.ustc.edu.cn:8080/PASmiR, and http://pcsb.ahau.edu.cn:8080/PASmiR. Conclusion: The PASmiR database provides a solid platform for collection, standardization, and searching of miRNA-abiotic stress regulation data in plants. As such this database will be a comprehensive repository for miRNA regulatory mechanisms involved in plant response to abiotic stresses for the plant stress physiology community.

Background: Essential Proteins are demonstrated to exert vital functions on cellular processes and are indispensable for the survival and reproduction of the organism. Traditional centrality methods perform poorly on complex protein–protein interaction (PPI) networks. Machine learning approaches based on high-throughput data lack the exploitation of the temporal and spatial dimensions of biological information. Results: We put forward a deep learning framework to predict essential proteins by integrating features obtained from the PPI network, subcellular localization, and gene expression profiles. In our model, the node2vec method is applied to learn continuous feature representations for proteins in the PPI network, which capture the diversity of connectivity patterns in the network. The concept of depthwise separable convolution is employed on gene expression profiles to extract properties and observe the trends of gene expression over time under different experimental conditions. Subcellular localization information is mapped into a long one-dimensional vector to capture its characteristics. Additionally, we use a sampling method to mitigate the impact of imbalanced learning when training the model. With experiments carried out on the data of Saccharomyces cerevisiae, results show that our model outperforms traditional centrality methods and machine learning methods. Likewise, the comparative experiments have manifested that our process of various biological information is preferable. Conclusions: Our proposed deep learning framework effectively identifies essential proteins by integrating multiple biological data, proving a broader selection of subcellular localization information significantly improves the results of prediction and depthwise separable convolution implemented on gene expression profiles enhances the performance.

The accurate prevention and control of pear tree diseases, especially the precise segmentation of leaf diseases, poses a serious challenge to fruit farmers globally. Given the possibility of disease areas being minute with ambiguous boundaries, accurate segmentation becomes difficult. In this study, we propose a pear leaf disease segmentation model named MFBP-UNet. It is based on the UNet network architecture and integrates a Multi-scale Feature Extraction (MFE) module and a Tokenized Multilayer Perceptron (BATok-MLP) module with dynamic sparse attention. The MFE enhances the extraction of detail and semantic features, while the BATok-MLP successfully fuses regional and global attention, striking an effective balance in the extraction capabilities of both global and local information. Additionally, we pioneered the use of a diffusion model for data augmentation. By integrating and analyzing different augmentation methods, we further improved the model’s training accuracy and robustness. Experimental results reveal that, compared to other segmentation networks, MFBP-UNet shows a significant improvement across all performance metrics. Specifically, MFBP-UNet achieves scores of 86.15%, 93.53%, 90.89%, and 0.922 on MIoU, MP, MPA, and Dice metrics, marking respective improvements of 5.75%, 5.79%, 1.08%, and 0.074 over the UNet model. These results demonstrate the MFBP-UNet model’s superior performance and generalization capabilities in pear leaf disease segmentation and its inherent potential to address analogous challenges in natural environment segmentation tasks.

Image datasets acquired from orchards are commonly characterized by intricate backgrounds and an imbalanced distribution of disease categories, resulting in suboptimal recognition outcomes when attempting to identify apple leaf diseases. In this regard, we propose a novel apple leaf disease recognition model, named RFCA ResNet, equipped with a dual attention mechanism and multi-scale feature extraction capacity, to more effectively tackle these issues. The dual attention mechanism incorporated into RFCA ResNet is a potent tool for mitigating the detrimental effects of complex backdrops on recognition outcomes. Additionally, by utilizing the class balance technique in conjunction with focal loss, the adverse effects of an unbalanced dataset on classification accuracy can be effectively minimized. The RFB module enables us to expand the receptive field and achieve multi-scale feature extraction, both of which are critical for the superior performance of RFCA ResNet. Experimental results demonstrate that RFCA ResNet significantly outperforms the standard CNN network model, exhibiting marked improvements of 89.61%, 56.66%, 72.76%, and 58.77% in terms of accuracy rate, precision rate, recall rate, and F1 score, respectively. It is better than other approaches, performs well in generalization, and has some theoretical relevance and practical value.

The advancement of society and technology has promoted the development of modern agriculture. It has become a trend to replace traditional manpower with intelligent agricultural machinery that operates independently. As the core technology of intelligent agricultural machinery, complete coverage path planning technology has become more important. At present, the complete coverage path planning algorithms still suffer from problems such as sacrificing the coverage rate to obtain the minimum energy consumption, taking a long time to calculate the algorithm, and destroying crops across the covered region. In view of the above problems, an improved complete coverage path planning algorithms based on backtracking is proposed combined with the actual needs of intelligent agricultural machinery for planting seedlings to improve four aspects: repeated coverage, search efficiency, path planning, and sub-regional crossing. Firstly, the Morse decomposition method is used to divide a complex farmland region into simple sub-regions. Then an improved backtracking method based on a greedy algorithm is proposed in order to reduce the computational efficiency of the current region connection algorithms. The priority principle and the strategy of moving along the boundary are used to solve the problems of region crossing and sacrificing the coverage rate, thereby improving the performance of the current complete coverage path planning method. Compared with the traditional backtracking method, the experimental results show that the number of backtracking points is decreased by about 70% and the occurrence of crossing sub-regions has been significantly reduced. This proposed method can improve the coverage and operating efficiency of intelligent agricultural machinery operations and provide technical support for agricultural operations such as sowing, tillage, and harvesting, thus improving the quality and efficiency of agricultural production.