Microalgae productive chains are gaining importance as sustainable alternatives to obtain natural pigments. This work presents a review on the most promising pigments and microalgal sources by gathering trends from a 10-year bibliometric survey, a patents search, and an industrial and market analysis built from available market reports, projects and companies’ webpages. The performed analysis pointed out chlorophylls, phycocyanin, astaxanthin, and β-carotene as the most relevant pigments, and Chlorella vulgaris, Spirulina platensis, Haematococcus pluvialis, and Dunaliella salina, respectively, as the most studied sources. Haematococcus is referred in the highest number of patents, corroborating a high technological interest in this microalga. The biorefinery concept, investment in projects and companies related to microalgae cultivation and/or pigment extraction is increasingly growing, particularly, for phycocyanin from Spirulina platensis. These pieces of evidence are a step forward to consolidate the microalgal pigments market, which is expected to grow in the coming years, increasing the prospects of replacing synthetic pigments by natural counterparts.

A theoretical simulator of immiscible displacement of a non-wetting fluid by a wetting one in a random porous medium is developed. The porous medium is modelled as a network of randomly sized unit cells of the constricted-tube type. Under creeping-flow conditions the problem is reduced to a system of linear equations, the solution of which gives the instantaneous pressures at the nodes and the corresponding flowrates through the unit cells. The pattern and rate of the displacement are obtained by assuming quasi-static flow and taking small time increments. The porous medium adopted for the simulations is a sandpack with porosity 0.395 and grain sizes in the range from 74 to 148 μrn. The effects of the capillary number, Ca, and the viscosity ratio, κ = μ_o/μ_w, are studied. The results confirm the importance of the capillary number for displacement, but they also show that for moderate and high Ca values the role of κ is pivotal. When the viscosity ratio is favourable (κ < 1), the microdisplacement efficiency begins to increase rapidly with increasing capillary number for Ca > 10⁻⁵, and becomes excellent as Ca → 10⁻³. On the other hand, when the viscosity ratio is unfavourable (κ > 1), the microdisplacement efficiency begins to improve only for Ca values larger than, say, 5 × 10⁻⁴, and is substantially inferior to that achieved with κ < 1 and the same Ca value. In addition to the residual saturation of the non-wetting fluid, the simulator predicts the time required for the displacement, the pattern of the transition zone, the size distribution of the entrapped ganglia, and the acceptance fraction as functions of Ca, κ, and the porous-medium geometry.

The awareness of sustainability approaches has focused attention on replacing synthetic emulsifiers with natural alternatives when formulating nanoemulsions. In this context, a comprehensive review of the different types of saponins being successfully used to form and stabilize nanoemulsions is presented, highlighting the most common natural sources and biosynthetic routes. Processes for their extraction and purification are also reviewed altogether with the recent advances for their characterization. Concerning the preparation of the nanoemulsions containing saponins, the focus has been initially given to screening methods, lipid phase used, and production procedures, but their characterization and delivery systems explored are also discussed. Most experimental outcomes showed that the saponins present high performance, but the challenges associated with the saponins' broader application, mainly the standardization for industrial use, are identified. Future perspectives report, among others, the emerging biotechnological processes and the use of byproducts in a circular economy context.