Elucidating the complex relationship between plants and endophytic fungi is very important in order to understand the maintenance of biodiversity, equity, stability, and ecosystem functioning. However, knowledge about the diversity of endophytic fungi from species of the native Brazilian Cerrado biome is poorly documented and remains largely unknown. These gaps led us to characterize the diversity of Cerrado endophytic foliar fungi associated with six woody species (Caryocar brasiliense, Dalbergia miscolobium, Leptolobium dasycarpum, Qualea parviflora, Ouratea hexasperma, and Styrax ferrugineus). Additionally, we investigated the influence of host plant identities on the structure of fungal communities. Culture-dependent methods coupled with DNA metabarcoding were employed. Irrespective of the approach, the phylum Ascomycota and the classes Dothideomycetes and Sordariomycetes were dominant. Using the cultivation-dependent method, 114 isolates were recovered from all the host species and classified into more than 20 genera and 50 species. Over 50 of the isolates belonged to the genus Diaporthe, and were distributed into more than 20 species. Metabarcoding revealed the phyla Chytridiomycota, Glomeromycota, Monoblepharomycota, Mortierellomycota, Olpidiomycota, Rozellomycota, and Zoopagomycota. These groups are reported for the first time as components of the endophytic mycobiome of Cerrado plant species. In total, 400 genera were found in all host species. A unique leaf endophytic mycobiome was identified in each host species, which differed not only by the distribution of fungal species, but also by the abundance of shared species. These findings highlight the importance of the Brazilian Cerrado as a reservoir of microbial species, and emphasize how endophytic fungal communities are diversified and adapted.

Mining significantly impacts terrestrial ecosystems despite its importance to the global economy. As part of soil ecosystems, fungi are highly responsive to environmental and human-induced drivers, shifting community composition and structure. Indeed, fungi play a key role in maintaining ecosystem resilience. Thus, we aim to address the question of whether soil fungal communities maintain similar ecological functions despite changes in community composition due to the impact of mining across ecosystems. To evaluate the ecological role of fungi across four ecosystems with varying iron mining impact levels, we used the FUNGuild database to assign functional guilds at the genus level. Co-occurrence network and ordination analyses were used to infer ecological relationships among fungal taxa and visualize the correlation between edaphic properties and fungal communities. A total of 22 functional guilds were identified, with dung saprotrophs, wood saprotrophs, fungal parasites, plant pathogens, ectomycorrhizal fungi, animal pathogens, and endophytes being the most abundant. Soil properties such as pH, organic matter, texture, and nutrients drive taxonomic and functional shifts. Our findings indicate that while mining activities shift fungal community compositions across ecosystems, the profiles of functional guilds show overlap between highly, moderately, and lowly impacted ecosystems, indicating functional redundancy. Network analysis reveals that highly connected hub taxa contribute to ecological redundancy across ecosystems and might act as a buffer against environmental disturbances. Our findings emphasize the important ecological role of soil fungi and indicate a potential for using fungal communities as bioindicators of ecological recovery in post-mining landscapes. From a mining and restoration perspective, this offers a low-cost, ecologically meaningful tool for monitoring soil recovery and guiding reclamation efforts.

The effect of agricultural practices on soil bacterial communities is not constant and depends a lot on the climatic context, changes in the soil characteristics, land use, and agricultural strategy. Thus, knowledge about how different land use systems in the Cerrado influence the diversity and taxonomic structure of microbial communities under the same soil type remains limited. In this context, the objective of this work was to analyze and compare the bacterial communities of Cerrado soil under two different land use systems (cover crop and potato cultivation) and in a neighboring native Cerrado area. For this, we used high-throughput amplicon sequencing of 16S rRNA genes (metabarcoding) to characterize the bacterial community at different taxonomic levels in a native Cerrado area, in a potato crop area, and in an area with cover crops. Our data indicated significant impacts on soil physicochemical properties and enzymatic activity, which directly reflect the dynamics of bacterial communities. The three bacterial phyla with the highest relative abundance in the three areas were Proteobacteria, Actinobacteriota, and Acidobacteriota. At the taxonomic class level, small variations were observed among areas, while at the amplicon sequence variant (ASV) level, these variations were more pronounced. The alpha diversity indices showed that the bacterial communities among the areas are rich and diverse. Bray–Curtis and Jaccard distance-based PCoA demonstrated an overlap of bacterial communities present in the cover crop area with the native Cerrado area and separation from the potato cultivation area. The in silico prediction demonstrated that the native Cerrado area presented the highest values of functional diversity of the soil bacterial community compared to the others. Thus, our results provide a holistic view of how different land use systems in the Cerrado can influence the taxonomic and functional diversity of soil bacterial communities.