Ewa Kowalska

Ms. Ewa Kowalska


Research Focus & Metrics

Academic Career Length

5Years (2022-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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21 May 2022 in Molecules
Verified
Journal Article
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EKEwa Kowalska
Ewa Kowalska
Malgorzata ZiarnoMalgorzata Ziarno
Malgorzata Ziarno
Adam EkielskiAdam Ekielski
Adam Ekielski
TŻTomasz Żelaziński
Tomasz Żelaziński
Probiotics and probiotic therapy have been rapidly developing in recent years due to an increasing number of people suffering from digestive system disorders and diseases related to intestinal dysbiosis. Owing to their activity in the intestines, including the production of short-chain fatty acids, probiotic strains of lactic acid bacteria can have a significant therapeutic effect. The activity of probiotic strains is likely reduced by their loss of viability during gastrointestinal transit. To overcome this drawback, researchers have proposed the process of microencapsulation, which increases the resistance of bacterial cells to external conditions. Various types of coatings have been used for microencapsulation, but the most popular ones are carbohydrate and protein microcapsules. Microencapsulating probiotics with vegetable proteins is an innovative approach that can increase the health value of the final product. This review describes the different types of envelope materials that have been used so far for encapsulating bacterial biomass and improving the survival of bacterial cells. The use of a microenvelope has initiated the controlled release of bacterial cells and an increase in their activity in the large intestine, which is the target site of probiotic strains.

11 April 2023 in Microorganisms
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PCPatrycja Cichońska
Patrycja Cichońska
EKEwa Kowalska
Ewa Kowalska
Malgorzata ZiarnoMalgorzata Ziarno
Malgorzata Ziarno
In recent years, scientists have been particularly interested in the gut–brain axis, as well as the impact of probiotics on the nervous system. This has led to the creation of the concept of psychobiotics. The present review describes the mechanisms of action of psychobiotics, their use in food products, and their viability and survival during gastrointestinal passage. Fermented foods have a high potential of delivering probiotic strains, including psychobiotic ones. However, it is important that the micro-organisms remain viable in concentrations ranging from about 106 to 109 CFU/mL during processing, storage, and digestion. Reports indicate that a wide variety of dairy and plant-based products can be effective carriers for psychobiotics. Nonetheless, bacterial viability is closely related to the type of food matrix and the micro-organism strain. Studies conducted in laboratory conditions have shown promising results in terms of the therapeutic properties and viability of probiotics. Because human research in this field is still limited, it is necessary to broaden our understanding of the survival of probiotic strains in the human digestive tract, their resistance to gastric and pancreatic enzymes, and their ability to colonize the microbiota.

28 March 2025 in Applied Sciences
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Journal Article
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Malgorzata ZiarnoMalgorzata Ziarno
Malgorzata Ziarno
DZDorota Zaręba
Dorota Zaręba
EKEwa Kowalska
Ewa Kowalska
Tomasz FlorowskiTomasz Florowski
Tomasz Florowski
The growing demand for plant-based and hybrid dairy–plant beverages has driven interest in optimizing their fermentation processes. This study investigates the effects of selected lactic acid bacteria (LAB) cultures on the quality characteristics of fermented dairy, dairy–oat, and oat beverages. The term ‘dairy-oat beverage’ refers to a hybrid product composed of cow’s milk and an oat-based drink in a 1:1 ratio. Cow’s milk, an oat beverage, and a 1:1 mixture of both were inoculated with traditional yogurt cultures (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and/or probiotic strains (Lactiplantibacillus plantarum 299v and Lactobacillus acidophilus La-5). Fermentation was conducted for 6 h at 37 °C, followed by 28 days of cold storage. pH, texture (hardness and adhesiveness), syneresis, carbohydrate content, and bacterial viability were assessed. The selection of lactic acid bacteria cultures had a significant impact on the quality attributes of the beverages. Both the bacterial culture type and the base material played a crucial role in determining the beverages’ texture, stability, and overall quality. Mixed bacterial cultures exhibited higher hardness, while milk and dairy–oat samples fermented with the yogurt culture demonstrated better structural stability. Fermentation influenced sugar levels, and bacterial viability depended on the beverage type and storage conditions. The selection of lactic acid bacteria cultures significantly impacts the quality of fermented beverages. Further optimization of bacterial culture combinations could improve these products’ stability and sensory properties.

15 March 2025 in Applied Sciences
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Journal Article
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Malgorzata ZiarnoMalgorzata Ziarno
Malgorzata Ziarno
DZDorota Zaręba
Dorota Zaręba
EKEwa Kowalska
Ewa Kowalska
Tomasz FlorowskiTomasz Florowski
Tomasz Florowski
In the context of the growing popularity of plant–based diets and the search for alternatives to traditional dairy products, there is a need to develop new, consumer–appealing products that combine the nutritional value of milk and oats. The aim of this study was to investigate the effects of different proportions of oat beverage in cow’s milk on the physicochemical, textural, sensory, and microbiological properties of fermented dairy–oat beverages, as well as to assess the stability of these properties during cold storage. Dairy–oat beverages with varying percentages of oat beverage (0–100%) were prepared and subjected to fermentation using starter cultures of S. thermophilus and L. delbrueckii subsp. bulgaricus. Changes in pH, lactose content, color, syneresis, texture, and starter bacteria population were evaluated over 28 days of storage at 6 °C. The results showed that the addition of oat beverage significantly affected the chemical composition, fermentation process, and quality characteristics of the final product. Changes were observed in the content of protein, fat, lactose, and carbohydrates, a slower fermentation process, changes in color, increased syneresis, and changes in texture depending on the proportion of oat beverage. The optimal proportions of the cow’s milk and oat beverage mixture, ensuring the desired sensory, textural, and microbiological stability, were found to be in the range of 25–50% oat beverage addition.

1 May 2024 in Preprints.org
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Malgorzata ZiarnoMalgorzata Ziarno
Malgorzata Ziarno
PCPatrycja Cichońska
Patrycja Cichońska
EKEwa Kowalska
Ewa Kowalska
DZDorota Zaręba
Dorota Zaręba
This study investigated a novel probiotic-enriched ice cream containing fermented white kidney bean homogenate to explore its potential health benefits. We assessed the viability of various probiotic strains during ice cream production and storage, focusing on their potential to reach the gut, and evaluated the overall antioxidant activity using DPPH, ABTS, FRAP, and TPC assays. The incorporation of fermented white bean homogenate significantly increased antioxidant capacity compared to the control group. Notably, strains like L. rhamnosus GG and L. plantarum 299v demonstrated the most pronounced effects on antioxidant activity, suggesting potential synergistic benefits between probiotics and bioactive compounds in fermented white beans. Although all probiotic strains experienced decreased viability during storage, certain strains, particularly L. plantarum 299v and L. casei DN-114001, showed promising survival rates even after 6 months. These results suggest the potential for developing probiotic ice cream containing viable bacteria capable of reaching the gut and contributing to a healthy gut microbiota. This could potentially improve digestion, reduce inflammation, and boost the immune system. Overall, this study highlights the potential of probiotic-enriched ice cream with fermented white kidney bean homogenate to combine the established benefits of probiotics for gut health with the enjoyment of consuming ice cream.