Fantao Kong

Dr. Fantao Kong

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China
orcid

Research Focus & Metrics

Academic Career Length

23Years (2004-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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23 February 2018 in New Phytologist
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Journal Article
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Fantao KongFantao Kong
Fantao Kong
IRIsmael Torres Romero
Ismael Torres Romero
JWJaruswan Warakanont
Jaruswan Warakanont
YLYonghua Li-Beisson
Yonghua Li-Beisson

20 March 2017 in The Plant Journal
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Journal Article
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Fantao KongFantao Kong
Fantao Kong
YLYuanxue Liang
Yuanxue Liang
BLBertrand Légeret
Bertrand Légeret
ABAudrey Beyly‐Adriano
Audrey Beyly‐Adriano
SBStéphanie Blangy
Stéphanie Blangy
YLYonghua Li-Beisson
Yonghua Li-Beisson

1 April 2020 in Journal of CO2 Utilization
Verified
Journal Article
Closed access
CZChenba Zhu
Chenba Zhu
XZXiaoqian Zhai
Xiaoqian Zhai
YXYimei Xi
Yimei Xi
JWJinghan Wang
Jinghan Wang
Fantao KongFantao Kong
Fantao Kong
ZCZhanyou Chi
Zhanyou Chi

11 July 2018 in The Plant Cell
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Journal Article
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Fantao KongFantao Kong
Fantao Kong
ABAdrien Burlacot
Adrien Burlacot
YLYuanxue Liang
Yuanxue Liang
BLBertrand Légeret
Bertrand Légeret
SASaleh Alseekh
Saleh Alseekh
YLYonghua Li-Beisson
Yonghua Li-Beisson
Plants and algae must tightly coordinate photosynthetic electron transport and metabolic activities given that they often face fluctuating light and nutrient conditions. The exchange of metabolites and signaling molecules between organelles is thought to be central to this regulation but evidence for this is still fragmentary. Here, we show that knocking out the peroxisome-located MALATE DEHYDROGENASE2 (MDH2) of Chlamydomonas reinhardtii results in dramatic alterations not only in peroxisomal fatty acid breakdown but also in chloroplast starch metabolism and photosynthesis. mdh2 mutants accumulated 50% more storage lipid and 2-fold more starch than the wild type during nitrogen deprivation. In parallel, mdh2 showed increased photosystem II yield and photosynthetic CO2 fixation. Metabolite analyses revealed a >60% reduction in malate, together with increased levels of NADPH and H2O2 in mdh2. Similar phenotypes were found upon high light exposure. Furthermore, based on the lack of starch accumulation in a knockout mutant of the H2O2-producing peroxisomal ACYL-COA OXIDASE2 and on the effects of H2O2 supplementation, we propose that peroxisome-derived H2O2 acts as a regulator of chloroplast metabolism. We conclude that peroxisomal MDH2 helps photoautotrophs cope with nitrogen scarcity and high light by transmitting the redox state of the peroxisome to the chloroplast by means of malate shuttle- and H2O2-based redox signaling.

15 January 2021 in Frontiers in Bioengineering and Biotechnology
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Journal Article
Open access
YXYimei Xi
Yimei Xi
Fantao KongFantao Kong
Fantao Kong
ZCZhanyou Chi
Zhanyou Chi
The unicellular alga Dunaliella salina is regarded as a promising cell factory for the commercial production of β-carotene due to its high yield of carotenoids. However, the underlying mechanism of β-carotene accumulation is still unclear. In this study, the regulatory mechanism of β-carotene accumulation in D. salina under stress conditions was investigated. Our results indicated that there is a significant positive correlation between the cellular ROS level and β-carotene content, and the maximum quantum efficiency (Fv/Fm) of PSII is negatively correlated with β-carotene content under stress conditions. The increase of ROS was found to be coupled with the inhibition of Fv/Fm of PSII in D. salina under stress conditions. Furthermore, transcriptomic analysis of the cells cultivated with H2O2 supplementation showed that the major differentially expressed genes involved in β-carotene metabolism were upregulated, whereas the genes involved in photosynthesis were downregulated. These results indicated that ROS induce β-carotene accumulation in D. salina through fine-tuning genes which were involved in photosynthesis and β-carotene biosynthesis. Our study provided a better understanding of the regulatory mechanism involved in β-carotene accumulation in D. salina, which might be useful for overaccumulation of carotenoids and other valuable compounds in other microalgae.

20 March 2019 in The Plant Cell
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Journal Article
Closed access
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YYYasuyo Yamaoka
Yasuyo Yamaoka
SSSeungjun Shin
Seungjun Shin
BCBae Young Choi
Bae Young Choi
HKHanul Kim
Hanul Kim
SJSunghoon Jang
Sunghoon Jang
YLYoungsook Lee
Youngsook Lee
Endoplasmic reticulum (ER) stress is caused by the stress-induced accumulation of unfolded proteins in the ER. Here, we identified proteins and lipids that function downstream of the ER stress sensor INOSITOL-REQUIRING ENZYME1 (CrIRE1) that contributes to ER stress tolerance in Chlamydomonas (Chlamydomonas reinhardtii). Treatment with the ER stress inducer tunicamycin resulted in the splicing of a 32-nucleotide fragment of a basic leucine zipper 1 (bZIP1) transcription factor (CrbZIP1) mRNA by CrIRE1 that, in turn, resulted in the loss of the transmembrane domain in CrbZIP1, and the translocation of CrbZIP1 from the ER to the nucleus. Mutants deficient in CrbZIP1 failed to induce the expression of the unfolded protein response genes and grew poorly under ER stress. Levels of diacylglyceryltrimethylhomoserine (DGTS) and pinolenic acid (18:3Δ5,9,12) increased in the parental strains but decreased in the crbzip1 mutants under ER stress. A yeast one-hybrid assay revealed that CrbZIP1 activated the expression of enzymes catalyzing the biosynthesis of DGTS and pinolenic acid. Moreover, two lines harboring independent mutant alleles of Chlamydomonas desaturase (CrDES) failed to synthesize pinolenic acid and were more sensitive to ER stress than were their parental lines. Together, these results indicate that CrbZIP1 is a critical component of the ER stress response mediated by CrIRE1 in Chlamydomonas that acts via lipid remodeling.