Fabaceae family is recognized as a prolific flavonoid producer, including some unusual flavonoid skeletons. Although classic flavonoids have well-established biological properties in the literature, these unusual compounds remain underinvestigated. Based on that, the current study sought to explore the chemistry and biological activity of rare flavonoids in Fabaceae with a review comprising their occurrence, extraction, isolation and pharmacological potential. Additionally, the use of LC-MS applied to the study of seven subclasses (aurones, biflavonoids, coumestans, homoisoflavonoids, neoflavonoids, pterocarpans, and rotenoids) is also discussed. The review was carried out by searching for specified uniterms on SciFinder and Web of Science, covering the last ten years. In addition, to assess ADMET and drug-like properties by in silico predictions, all the substances considered in this work were compiled and organized into a curated dataset. As a result, a total of 170 flavonoid structures were included, most of which were found in the roots and leaves. In addition, a wide range of biological activities were observed, such as cytotoxic, antiviral and anti-inflammatory. Despite advancements in the use of LC-MS for bioprospection, just a few reports dedicated to the study of these compounds were found. Regarding ADMET profiles, most subclasses showed favorable bioavailability characteristics, with biflavonoids being the main exception. Together, this review highlights the significance of unusual flavonoids from the Fabaceae family, demonstrating their remarkable chemical diversity and largely, but still, unexplored pharmacological potential. These findings encourage further investigations, particularly in the fields of natural products chemistry, medicinal chemistry, and pharmacology.

Cordia rufescens A. DC, a small shrub in northeastern Brazil is popularly referred to as "old-growth" or "pigeon-claw". This plant is used in folk medicine as abortive, anti-inflammatory agent and in the treatment of dysmenorrhea and dyspesia (VALE et al., 2012). Thus, the need for a greater chemical and pharmacological knowledge of Cordia rufescens A.DC species was observed, necessitating a more in-depth study of the isolation and characterization of new compounds. The plant material (leaves) was collected in 2002 in the municipality of Cruz do Espírito Santo - PB and identified by Profa. Dr. Maria de Fátima Agra. The material was then oven dried with circulating air at an average temperature of 45 ° C, ground in a mechanical mill and subjected to steeping with 95% EtOH. The crude ethanolic extract (106.10 g) was dissolved in a methanol: water (7: 3 v / v) solution and partitioned with the following solvents: hexane, dichloromethane and ethyl acetate. An aliquot of the dichloromethane phase (7 g) was subjected to the chromatographic column (CC), using as a stationary phase silica gel, and as mobile phase hexane, ethyl acetate and methanol, with elution systems in increasing polarity order, obtaining 34 fractions. All fractions were submitted to analytical thin layer chromatography (ADCC) and analyzed and assembled according to their retention factors (Rfs) and after visualization in ultraviolet light. Fractions 2, 3 and 5 presented different retention factors and therefore were submitted to liquid chromatography of high efficiency in analytical scale, where fraction 5 was chosen to obtain a better resolution of the chromatogram in front of the others, being then submitted to the purification by HPLC on a semi-preparative scale that resulted in the identified substances 4-hydroxy-benzaldeído and 4 -hydroxy-3,5-dimethoxycinnamate, reported for the first time in the species under study.

Book Brazil in Advancing Pharmacotherapy and Clinical Pharmacy in Latin America Proceedings of the Fourth Brazilian Congress of Pharmacotherapy and Clinical Pharmacy doi: 10.62922/4cbffc ISBN: 978-65-983944-2-4 Editors Wallace Entringer Bottacin – ORCID | Lattes | Google Scholar Thaís Teles de Souza – ORCID | Lattes Walleri Christini Torelli Reis – ORCID | Lattes | Google […]

Erythroxylum rimosum O. E. Schulz is a species restricted to the northeastern region of Brazil, found in the states of Ceará, Piauí, Sergipe and Bahia, occurring respectively in Restinga, Cerrado and Carrasco vegetation. The study of the extract of E. rimosum, reported the identification of pentacyclictriterpenes, steroid, tropic alkaloid and flavonoids. Thus, a chromatographic study of its crude ethanol extract was carried out. The botanical material of the aerial parts was collected in the municipality of Pirambu, state of Sergipe and identified by Profa. Dr. Ana Paula do Nascimento Prata, Department of Biology, Federal University of Sergipe (FUS). It was then oven dried with circulating air at an average temperature of 40 ° C, ground in a mechanical mill and subjected to steeping with 95% EtOH. The BSE (105 g) was dissolved in a methanol: water (7: 3 v / v) solution and partitioned with the following solvents: hexane, dichloromethane and ethyl acetate. The AcOEt phase was subjected to column chromatography, using silica gel 60 as stationary phase and as mobile phase, the Hex, AcOEt and MeOH solvents, pure and in binary mixtures in increasing order of polarity. This yields 30 fractions which after analytical thin layer chromatography (TLC) were pooled according to their respective retention factors (Rfs). The fractions from 23 to 25 were submitted to High Performance Liquid Chromatography Coupled to a Diode Array Detector (HPLC-DAD). Getting yourself 8 fractions. From fractions 4 and 2 the coded substances of Er-1 and Er-2 respectively were obtained. They have had their structures identified by ¹H-NMR, ¹³C, and two-dimensional techniques in comparison with literature data, namely: kaempferol-3-rutinoside and quercetin-3-O-β-D-glucopyranoside-α- L-raminoside, two glycosylated flavonoids that are being reported for the first time in the study species.