Kaff-e-Maryam (Anastatica hierochuntica L.) is extensively used to treat a range of health problems, most notably to ease childbirth and alleviate reproductive system-related disorders. This study aimed to evaluate the effect of A. hierochuntica ethanolic (KEE), and aqueous (KAE) extracts on CCl₄-induced oxidative stress and nephrotoxicity in rats using the biochemical markers for renal functions and antioxidant status as well as histopathological examinations of kidney tissue. A. hierochuntica contained 67.49 mg GAE g⁻¹ of total phenolic compounds (TPC), 3.51 µg g⁻¹ of total carotenoids (TC), and 49.78 and 17.45 mg QE g⁻¹ of total flavonoids (TF) and total flavonols (TFL), respectively. It resulted in 128.71 µmol of TE g⁻¹ of DPPH-RSA and 141.92 µmol of TE g⁻¹ of ABTS-RSA. A. hierochuntica presented superior antioxidant activity by inhibiting linoleic acid radicals and chelating oxidation metals. The HPLC analysis resulted in 9 and 21 phenolic acids and 6 and 2 flavonoids in KEE and KAE with a predominance of sinapic and syringic acids, respectively. Intramuscular injection of vit. E + Se and oral administration of KEE, KAE, and KEE + KAE at 250 mg kg⁻¹ body weight significantly restored serum creatinine, urea, K, total protein, and albumin levels. Additionally, they reduced malondialdehyde (MOD), restored reduced-glutathione (GSH), and enhanced superoxide dismutase (SOD) levels. KEE, KAE, and KEE + KAE protected the kidneys from CCl₄-nephrotoxicity as they mainly attenuated induced oxidative stress. Total nephroprotection was about 83.27%, 97.62%, and 78.85% for KEE, KAE, and KEE + KAE, respectively. Both vit. E + Se and A. hierochuntica extracts attenuated the histopathological alteration in CCl₄-treated rats. In conclusion, A. hierochuntica, especially KAE, has the potential capability to restore oxidative stability and improve kidney function after CCl₄ acute kidney injury better than KEE. Therefore, A. hierochuntica has the potential to be a useful therapeutic agent in the treatment of drug-induced nephrotoxicity.

Antioxidative, antidiabetic, and hypolipidemic properties of probiotic-enriched fermented camel milk (FCM) combined with Salvia officinalis L. leaves hydroalcoholic extract (SOHE) in streptozotocin-induced diabetes in rats were investigated. Phytochemicals analysis and antioxidant capacity indicated that S. officinalis contained high phenolics with super antioxidant activity. Subsequently, HPLC analysis demonstrated 13 phenolic acids and 14 flavonoids in considerable amounts with ferulic acid and resveratrol as predominant, respectively. The antidiabetic and hypolipidemic properties of FCM and SOHE were examined in a designed animal model consisting of seven treated groups for four weeks. There was a negative group (G1); the positive group (G2) received a single dose (50 mg kg⁻¹) of streptozotocin (STZ) by intraperitoneal injection (i.p.); in G3, diabetic rats (DRs) orally received 5 mL FCM kg⁻¹ daily; in G4, DRs orally received 50 mg GAE SOHE kg⁻¹ daily; in G5, DRs orally received 5 mL FCM contains 25 mg GAE SOHE kg⁻¹ daily; in G6, DRs orally received 5 mL FCM contains 50 mg GAE SOHE kg⁻¹ daily; in G7, DRs orally received 50 mg metformin kg⁻¹ daily. Combining FCM with SOHE at 25 or 50 mg kg⁻¹ exhibited a synergistic effect in significantly lowering random blood glucose (RBG), fasting blood glucose (FBG), and improved weight gain recovery %. The hypolipidemic effect of FCM + 50 mg GAE SOHE kg⁻¹ was significantly higher than using FCM or SOHE individually, and attenuation in triglycerides (TG), total cholesterol (CHO), and high- and low-density lipoproteins (HDL and LDL), and very-low-density lipoproteins (VLDL) was remarked. Combining FCM with SOHE at 25 or 50 mg kg⁻¹ ameliorated liver and kidney functions better than individual uses of FCM, SOHE, or metformin. Interestingly, FCM with 50 mg SOHE kg⁻¹ presented significant improvement in the activity of antioxidant enzymes, reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and a substantial reduction in malonaldehyde (MDA) levels with 53.75%, 89.93%, 63.06%, and 58.69% when compared to the STZ group (G2), respectively. Histopathologically, administrating FCM + 25, 50 mg SOHE kg⁻¹ or 50 mg kg⁻¹ metformin showed a normal histological structure of both islets of Langerhans cells and acini. In conclusion, combining FCM with SOHE presented synergistic and therapeutical efficacy. It could be beneficial and profitable for controlling diabetes mellitus complications and protecting against oxidative stress.

The high mortality of OvCa is caused by the wide dissemination of cancer within the abdominal cavity. OvCa cells metastasize to the peritoneum, which is covered by mesothelial cells, and invade into the underlying stroma, composed of extracellular matrices (ECM) and stromal cells. In a study using a three-dimensional quantitative high-throughput screening platform (3D-qHTS), we found that β-escin, a component of horse chestnut seed extract, inhibited OvCa adhesion/invasion. Here, we determine whether β-escin and structurally similar compounds have a therapeutic potential against OvCa metastasis. Different sources of β-escin and horse chestnut seed extract inhibited OvCa cell adhesion/invasion, both in vitro and in vivo. From a collection of 160 structurally similar compounds to β-escin, we found that cardiac glycosides inhibited OvCa cell adhesion/invasion and proliferation in vitro, and inhibited adhesion/invasion and metastasis in vivo. Mechanistically, β-escin and the cardiac glycosides inhibited ECM production in mesothelial cells and fibroblasts. The oral administration of β-escin inhibited metastasis in both OvCa prevention and intervention mouse models. Specifically, β-escin inhibited ECM production in the omental tumors. Additionally, the production of HIF1α-targeted proteins, lactate dehydrogenase A, and hexokinase 2 in omental tumors was blocked by β-escin. This study reveals that the natural compound β-escin has a therapeutic potential because of its ability to prevent OvCa dissemination by targeting both cancer and stromal cells in the OvCa tumor microenvironment.

The present study was carried out to evaluate the effects of partial replacement of soybean with shrimp by-products (SBp) in African catfish (Clarias lazera) diets on productive (growth, digestibility, body composition, dressing yield, blood parameters, immunity) and economic performances. Therefore, 750 fingerlings (~20.0 g) were distributed into five groups of 150 fingerlings/group with three replicates (n = 50) per group. Group 1 was fed a basal diet, while G2 and G3 were fed diets containing 25% SBp (autoclaved and acid-treated, respectively) as soybean replacer for 12 weeks, while, in G4 and G5, SBp level was 50%. All growth and nutrient utilization parameters (body weight, Wg, ADG, PER, FCR, and digestibility), plasma proteins, and immunity significantly increased in G4 and G5. Anemia screening markers recorded insignificant differences between the tested groups and control one, whereas lipid markers of plasma and fish body decreased by autoclaved SBp and increased by acid-treated SBp. The African catfish body dry matter and crude protein percentages were enriched by the addition of SBp. Replacing the soybean meal decreases diet costs and diet costs/1 kg of weight gain. In conclusion, replacing soybean with SBp (especially 50% acid-treated) positively influenced productive and economic performances with friendly effects to avoid the environmental pollution by these wastes.

Immunity is a major concept in human nutrition. Immunocompromised individuals are at risk for serious infection as COVID-19 which is directed the researchers to use the immunomodulatory plants for prophylaxis. This study was designed to assess the immune response of Wistar rats administrated Achillea fragrantissima (A. fragrantissima) extract versus Endoxan (immunosuppressive) and Echinaid (immune stimulating). Fifty rats were assigned into 5 groups: (1) control, (2) injected intraperitoneal (i/p) with Endoxan 90 mg/kg for three successive days, (3) injected with Endoxan as (2) and administrated with Echinaid 300 mg/kg/day. (4,5) injected with Endoxan as (2) and administrated with 300 and 500 mg/kg/day A. fragrantissima extract respectively. On day 17 all groups were challenged with two doses of sheep erythrocytes (SRBC) i/p, 2 weeks intervals. A high dose of A. fragrantissima extract achieved an increase in total antioxidant capacity significantly(P<0.05), superoxide dismutase, and a decrease in malondialdehyde. Catalase exerted a significant increase with a low dose of A. fragrantissima whereas a high dose had a mild effect. Echinaid and A. fragrantissima raised IgM for the first dose of SRBC and Igs and IgG for the second dose significantly (P<0.05). A. fragrantissima administration ameliorates cytokines (TNF-α, IL-4) and modulated IL-10 significantly (P<0.05). A high dose of A. fragrantissima extract exerted a significant reduction in splenic non-cellular viability% and the highest score of the microscopic immune reaction (immunostimulation++++). Splenic histopathology confirmed the present results. The current study highlights that a high dose of A. fragrantissima extract is preferred over a low dose to restore immune responses in vivo.

The objective of this study was to investigate the impact of including hydroxytyrosol (HT) antioxidant on the viability of sperm after the processes of cooling and freezing. HT antioxidants were used in the HF‐20 extender at concentrations of 1.25, 2.5, 5, and 10 μg/ml. The HF‐20 extender was a basic extender and was used for the control group. The post‐thawed semen exhibited significantly higher total motility in the 2.5 HT and 10 HT treatment groups than the control group. The straight line velocity (VSL) of the 2.5 HT group exhibited a significantly high value compared with the control group. However, the average path velocity (VAP), linearity (LIN), straightness index (STR), and wobble (WOB) revealed identical findings across all groups. The findings of the analysis of HOST, normal morphology, major abnormalities, and minor abnormalities revealed that there were no significant differences between the HT groups and the control groups. Nevertheless, the use of HT antioxidant for freezing semen led to a notable enhancement (p < 0.05) in both acrosome integrity and vitality tests when compared to the control group. In this case, the lower quantities of HT (1.25 and 2.5 μg/ml; p < 0.05) preserve the DNA fragmentation compared with the 5 HT, 10 HT, and control groups. In conclusion, the HT antioxidant has shown the capacity to enhance the quality of frozen‐thawed spermatozoa and positively influence the viability and integrity of DNA inside the frozen‐thawed spermatozoa. Additional research should be conducted to assess the fertility potential of cryopreserved stallion semen.