Honey is increasingly recognized not only as a functional food but also as a potential bioindicator of environmental pollution. This study assessed the concentrations of four potentially toxic elements (PTEs)—lead (Pb), cadmium (Cd), copper (Cu), and zinc (Zn)—in 48 multifloral honey samples collected in 2023 from seven locations across a historically polluted agro-industrial region in Romania. Samples were analyzed using Flame Atomic Absorption Spectrometry (FAAS) and Graphite Furnace AAS (GFAAS), with quality control ensured through certified reference materials. Results revealed that Pb (0.72–1.69 mg/kg) and Cd (0.02–0.37 mg/kg) levels consistently exceeded international safety thresholds, while Cu (0.62–2.22 mg/kg) and Zn (0.91–1.93 mg/kg), although essential nutrients, were found in elevated concentrations. Spatial analysis indicated a general trend of higher contamination in sites located closer to former industrial facilities, influenced by factors such as altitude and atmospheric transport. These findings confirm the persistent environmental burden in post-industrial landscapes and support the use of honey as a cost-effective tool for pollution monitoring. The study underscores the need for targeted environmental policies, sustainable apicultural practices, and continued surveillance to protect ecosystem health and food safety.

Erosion caused by human activities is one of the reasons for forest soil degradation worldwide, with a direct impact on forest stands development, including reduced forest productivity. Therefore, in order to establish sustainable stand management practices, it is essential to assess soil losses in various forestry activities. Moreover, this phenomenon is studied little in stands, especially those established on degraded land. In Romanian geographical conditions, where sloping land is up to 67% of the territory and is influenced by natural factors as well as intense human activities, the soil and vegetation suffer serious ecological imbalances. In order to achieve the proposed objectives regarding the evaluation of stands in terms of anti-erosion effectiveness, we analyzed the consistency and the number of trees on the surface, the weight of the seedlings, and the surface runoff from the perspectives of rainfall and soil retention. In the two stands included in this study, the influence of rain intensity was 39% in compartment 49 and 38% in compartment 73, directly influencing surface runoff. The ground retention’s influence on surface runoff was 28% in both compartments. The indirect surface runoff was influenced by the consistency of the stands and by the degree of proximity of the crowns, which directly influenced the intensity of rain. In addition to analyzing these two parameters (rain intensity and ground retention), it was also observed that the degree of proximity to the crowns directly influenced the intensity of the rainfall within the forest, which, in turn, indirectly affected the runoff.

The genetic variability of five populations of Quercus petraea originating from the Transylvania and Maramureș regions of Romania was investigated in this study to provide insights into the species’ adaptability, population dynamics, and potential for preservation in the face of environmental challenges. To achieve this, sequence-related amplified polymorphism (SRAP) markers, in conjunction with a set of 18 primer combinations, were employed. The outcomes of the analysis revealed a range of polymorphisms spanning from 69.78% to 85.75%. Additionally, the assessment of genetic diversity using Shannon’s information index (I) yielded values ranging between 0.2887 and 0.3955, while Nei’s gene diversity (He) exhibited a spectrum from 0.1833 to 0.2582. The analysis of genetic variability, conducted via molecular variance (AMOVA), unveiled that 9% of the genetic variation was attributable to differences among the populations, while a substantial 91% resided within the populations. A further investigation of the population structure revealed that the construction of a UPGMA dendrogram based on Nei’s genetic distances elucidated the presence of two principal genetic clusters, a finding that was reinforced by a Principal Coordinate Analysis (PCoA). The genetic diversity revealed by Quercus petraea using SRAP molecular markers offers promising potential for upcoming breeding programs to identify optimal genitors, facilitating the development of well-adapted oak populations in the Transylvania and Maramureș regions.

Oak forests provide critical ecosystem services, but are being increasingly exposed to climate variability, drought, and insect outbreaks that threaten their long-term resilience. This study aims to integrate structural canopy indicators with climate-derived indices to detect early-warning signals of decline in temperate oak stands. We monitored eight Forest Management Units in western Romania between 2017 and 2021, combining field-based assessments of crown morphology, vitality traits, defoliation, and epicormic shoot frequency with hydroclimatic indices such as the Forest Aridity Index. Results revealed strong spatial and temporal variability: several stands showed advanced canopy deterioration characterized by increased defoliation, dead branches, and epicormic resprouting, while others maintained stable conditions, suggesting resilience and suitability as reference sites. Insect defoliators, particularly Geometridae, contributed additional stress, but generally at subcritical levels. By synthesizing these metrics into conceptual models and a risk scorecard, we identified the causal pathways linking climatic anomalies and biotic stressors to structural decline. The findings demonstrate that combining structural and climatic indicators offers a transferable framework for forest health monitoring, providing robust early-warning tools to guide adaptive silviculture and resilience-based management. Beyond the Romanian context, this integrative approach supports sustainability goals by strengthening conservation strategies for temperate forests under global change.

Understanding the pathways through which heavy metals accumulate in medicinal plants and enter herbal infusions is essential for linking environmental quality with human exposure. This study investigated multi-matrix metal transfer in Tilia spp. along an urban–forest gradient by quantifying twelve elements (Pb, Cd, Zn, Cu, Ni, Cr, Mn, Co, As, Hg, Al, and V) in soil, bark, leaves, flowers, and corresponding infusions using inductively coupled plasma mass spectrometry and by estimating daily intake for different age groups based on EFSA default body weights and two consumption scenarios (150 and 400 mL day⁻¹). The results revealed clear spatial patterns, with significantly higher metal loads in urban sites and a consistent transfer from environmental compartments to plant tissues and infusions. Mn, Al, Pb, and Cd exhibited the highest extractability, leading to elevated estimated daily intakes in young children, identified as the most vulnerable group due to their lower body mass. However, all exposure values remained below EFSA and JECFA toxicological reference limits, while As and Hg were undetectable in all infusions. These findings indicate that Tilia infusions contribute minimally to overall dietary metal exposure and confirm Tilia spp. as reliable bioindicators of soil- and airborne metal deposition, supporting the safe consumption of linden tea under realistic intake conditions.

The hereby research aimed to identify the influence of several biostimulants on the germination capacity of pedunculate oak (Quercus robur L.), with the goal of enhancing the germination process, which is fundamental for the conservation and development of this ecologically and economically important species. Biological materials were collected from four different geographical provenances in Romania: Prejmer (Brașov), Racășa (Maramureș), Căpâlna (Alba), and Orăștie (Hunedoara). The main phenotypic characteristics of the seeds were analyzed, including length, diameter, and weight. Germination capacity was assessed using different biostimulants, compared with untreated seeds as a control. Results showed significant differences between the investigated provenances regarding morphological traits of seeds, respectively, seed length, diameter, and weight. Additionally, significant differences in germination capacity were noticed among the applied treatments and studied provenances. The treatment with Atonik biostimulant illustrated the best results within all provenances, followed by Nitrozym and Cropmax. The study offers useful information into enhancing the germination capacity of Q. robur, highlighting the significance of provenance and stimulation treatments for effective germination, significant for the conservation and sustainable management of this species.