The aim of this study was to investigate the occurrence of two heavy metals, lead and cadmium, in European hare liver samples, collected in agro biotope of northern Serbian province Vojvodina. Heavy metals such as lead (Pb) and cadmium (Cd) do not have any biological function in the animal body; however, they can be found due to the pollution in the environment. For the purpose of this study, in 196 samples from 17 different locations hare livers were analyzed for the occurrence of lead and cadmium. All samples were taken from hares harvested during the regular hunting season. The average value for lead in all analyzed samples was 884 µg/kg fresh weight (fw), with the range 59–3700 µg/kg fw. Only samples from two locations had the average concentration of lead which was within the permitted limit by the Serbian regulation. The average cadmium level in all samples was 243 µg/kg fw. The range of all samples was from 0 to 1414 µg/kg fw. Our research indicates that out of two investigated heavy metals, the occurrence of lead is more common and at a higher concentration in the agricultural development region of Vojvodina.

Summary: Lead (Pb) and cadmium (Cd) are heavy metals considered possible contaminants of water, soil and air (especially when occurring as a consequence of human activities in agriculture or industry), and can be found in the liver or kidneys of animals, thus serving as indicators of pollution. Vojvodina is a northern province of the Republic of Serbia which boasts very intensive agricultural production, especially crop production. The brown hare is well-adapted and abundant in Vojvodina. As a complete herbivore, it has the potential to be a good biomonitor for the presence of lead and cadmium in the chosen biotope. A total of 60 samples of hare livers were collected and analyzed immediately after hunting. The average concentration of lead in all the liver samples analyzed was 851.43 µg/kg of wet tissue. The lead concentrations determined at three different sites in Vojvodina (namely Odžaci in Bačka, Rusko selo in Banat and Kraljevci in Srem) were not statistically significant. However, the average lead concentrations at all the sites were above the permitted level (500 µg/kg of wet tissue). The maximum amount of lead determined was 2,302 µg/kg of wet tissue, i.e. almost five times above the permitted level. The cadmium concentrations in all the samples analyzed ranged from 12.90 to 1183.00 µg/kg of wet tissue. The average cadmium concentrations determined at three different sites were found statistically significant, suggesting a local source of contamination. The average cadmium concentrations at sites I, II and III were 65.57, 205.91 and 413.99 µg/kg of wet tissue, respectively. The average cadmium concentrations determined at the sites considered were below the permitted level, although exceeding the legal limit in some of the samples analyzed.

The research was conducted during the 2018/19 hunting year at three hunting grounds: "Barajevska reka" - "Takovo" and "Jadar". The average density of Roe deer in hunting grounds varied from 48.67 to 74 individuals per 1,000 ha of hunting-productive area. However, when observing the number of individuals per 1,000 ha of total hunting area, the density varies from 16.07 individuals to 34.72 individuals. The determined gender ratio at the hunting ground "Jadar" was (M: F = 1: 0.93). At the hunting ground "Takovo", the gender ratio was (M: F = 1: 1.22), while at the hunting ground "Barajevska reka" the ratio was (M: F = 1: 1.59). The average fertility of Roe deer was 1.67 embryos per individual, or 1.75 embryos per pregnant female. Depending on the study area, the real growth varied from 0.54 to 0.73 fawns per female. Loss in Roe deer are caused mostly by biotic factors. The determined average age of shot individuals varied depending on the hunting ground from 3.53 to 5.24 years. The current state of Roe deer populations in Serbia varies between analyzed hunting grounds, but the overall situation is quite unsatisfactory, especially in terms of density, gender ratio and age structure of culled individuals.

Summary: The purpose of this study is to examine the status of roe deer population in the hunting grounds of Central Serbia, and perform a SWOT analysis of its management. The data used herein on the number of roe deer individuals and culling events were obtained from the Ministry of Agriculture, Forestry and Water Management - Forest Administration and the Statistical Office of the Republic of Serbia. Moreover, the Law on Game and Hunting was used for analysis purposes. The status of roe deer population in the hunting grounds of Serbia is quite unsatisfactory, depending on the geographical area and wildlife management practices. In 2021, a total of 86,755 individuals were recorded in the area of Central Serbia, which accounts for 97.54% of the optimal population. However, the number of roe deer in the individual hunting grounds of the Central and Eastern hunting areas was above the optimal density, whereas the number of roe deer accounted for 88.58% and 89.12% of the optimal density in the individual hunting grounds of the Southern and Western hunting areas, respectively. The largest number of individuals per unit area (km2) was recorded in the Eastern (2.06) and Belgrade (2.02) hunting areas, whereas the smallest number of individuals per unit area (km2) was recorded in the Southern (1.02) and Western (1.34) hunting areas. The following issues were identified as major impediments to the management of roe deer population in the hunting grounds considered: legislative and regulatory instruments, non-compliance and non-enforcement of a large number of regulations, roe deer monitoring issues, poor hunting ground control, inapplicability of certain regulations in the field of veterinary medicine, environmental protection, unresolved issues of trophy and game meat export, lack of strategic planning (namely the hunting development strategies of the Republic of Serbia), lack of deer habitat monitoring methodology and rating methodology, and unprofessional planning and development of hunting ground management plans. The SWOT analysis performed emphasized the strengths, weaknesses, opportunities and threats of roe deer population management in Serbia.