The present investigation was undertaken to standardize the media and the anti-browning regime in order to minimize the phenolic browning of an in vitro culture of Persian walnut cv. Sulaiman. The experiments involved two types of explants, forced and unforced shoot tips, two types of media, Driver and Kuniyuki Walnut (DKW) medium andMurashige and Skooģs (MS) medium, and three types of anti-browning agents, namely, Polyvinylpyrrolidone, ascorbic acid and activated charcoal at 150, 350 and 550 mg/L each. The investigation was replicated thrice under a completely randomized design. Forced shoot tips of cv. Sulaiman on DKW medium showed the best performance in terms of least browning (13.6 ± 10.5%) and highest survival percentage of explants (74.5 ± 2.4%) when treated with ascorbic acid at 550 mg/L. However, unforced shoot tips in MS medium did not perform well and manifested maximum browning (52.9 ± 5.2%). Based on the results, we conclude that incorporation of ascorbic acid in the DKW medium significantly reduced the media and explant browning, thus, it could set the basis of successful in vitro-propagation of walnuts.