This study aimed to investigate the effects of aquaculture and the optimum conditions for drying duckweed plants to maintain the highest nutritional value and bioactive substances. Protein quantification was used to screen duckweed plants subjected to the 14 treatments under aquaculture conditions. Proximate analysis of three aquaculture conditions showed the highest quantification of protein. Moreover, these samples were analyzed for total phenolics, flavonoids, and chlorophylls. The optimal drying conditions for duckweed plants with the highest protein content were determined using a factorial design with three temperature and time parameters.

This study aimed to comprehensively evaluate the potential health benefits of Thai basil extracts from two species, Ocimum basilicum var. thyrsiflorum and Ocimum basilicum cv. Jumbo 4320, by investigating their bioactivities, phytochemical composition, and in vitro antioxidant, antimicrobial, and antithrombotic activities. Thai basil extracts from two species (Ocimum basilicum var. thyrsiflorum and cv. Jumbo 4320) were obtained using water, ethanol, and ethyl acetate. Phytochemical analysis revealed chlorophylls, carotenoids, and diverse phenolic compounds. Its water extract boasted the highest total phenolics (459.62 ± 3.07 mg GAE/100 g), outperforming ethanol and ethyl acetate extracts (171.20 ± 1.10 and 66.02 ± 0.99 mg GAE/100 g, respectively). The ethanol extract of Jumbo 4320 also reigned supreme in total flavonoids (557.12 ± 14.27 mg CE/100 g), surpassing its counterparts (209.07 ± 23.31 and 131.41 ± 0.37 mg CE/100 g). O. basilicum cv. Jumbo 4320 extracts exhibited superior antioxidant activity by various assays. Jumbo 4320′s water extract further dominated in the DPPH assay (IC₅₀ 48.52 ± 1.15 mg/mL), demonstrating superior free radical scavenging compared to ethanol and ethyl acetate extracts (IC₅₀ 60.54 ± 0.52 and 82.09 ± 3.18 mg/mL), respectively. While thyrsiflorum’s ethanol extract claimed the top spot in the FRAP assay (0.0186 ± 0.00 mmol Fe²⁺/g), Jumbo 4320′s water extract showcased the highest cellular antioxidant activity (80.62 ± 0.00% relative fluorescence intensity) in the DCFH-DA assay. Interestingly, Ocimum basilicum var. thyrsiflorum extracts demonstrated stronger antithrombotic activity at prolonging the prothrombin time at 78.3 ± 17.56 s. While the antimicrobial activity against most tested pathogens was limited, both species’ extracts inhibited Bacillus cereus. These findings suggest the potential of Thai basil extracts, particularly from Jumbo 4320, as functional food ingredients with antioxidant and antithrombotic properties.

Plant polysaccharides are used in the food industry to improve the texture and stability of food. The viscosity of polysaccharides, which includes both thickening and gelling, is an important characteristic. Yanang, Tilaicora triandra (Colebr.) Diels., composed of polysaccharide gum in its leaves. In this research, Yanang gum’s structural and rheological properties were investigated. The gum’s structure is xylan, with a backbone made up mostly of mixed (1,3)- and (1,4)-D-xylan. The average molecular weight of Yanang gum is 3819 kDa, with a gyration radius of 120.4 nm and an intrinsic viscosity of 14.6 dL/g. The power-law model was found to be the best fit for Yanang gum flow curves. The consistency coefficient, k, increases significantly with concentration in both the forward and the reverse measurements, whereas the flow behavior index, n, decreased as concentration increased. Yanang gum exhibited shear-thinning flow behavior. Increasing the concentration results in heightened G′ and G″, and the cross-over point shifts toward lower frequencies. The results of this study show that Yanang gum may be beneficial as other natural gums for food products.

The dietary fiber extracted from cassava pulp, composed of crude fiber, neutral detergent fiber (NDF), and cellulose content, demonstrates promise as a functional food ingredient. The study’s objectives encompassed the assessment of short-term toxicity and the evaluation of its potential cholesterol-lowering effects. The results indicated that cassava pulp dietary fiber (CDF) is well-tolerated with non-toxic thresholds determined at 10.01 g/kg body weight/day for male rats and 11.21 g/kg body weight/day for female rats during the short-term toxicity assessment. Furthermore, CDF exhibited notable cholesterol-lowering effects, significantly reducing serum triglyceride and serum total cholesterol levels, along with decreased liver total lipids and liver cholesterol levels. In contrast, it led to significant increases in fecal total lipids and cholesterol when compared to the control group. Most notably, there were no significant differences in terms of serum triglyceride, serum total cholesterol, liver total lipids, and liver cholesterol between CDF and the conventional cholesterol-lowering medication, simvastatin. These findings underscore the potential of cassava pulp dietary fiber as a natural and safe alternative for managing hyperlipidemia and related conditions. It offers a valuable avenue for the development of functional foods aimed at improving cardiovascular health and further investigation for its potential application in the field of nutraceuticals.

Rang Chuet (Thunbergia laurifolia Lindl.) is a Thai medicinal plant with pharmacological properties: it can be used as an antidote, for decreasing body temperature and it is addictive. This study investigated total phenolic contents and antioxidant activities of Rang Chuet extracts, and its acute and sub-chronic toxicities of Rang Chuet extracts. To investigate the acute toxicity of Rang Chuet, male and female Wistar rats were orally gavaged with a single dose of 2000 and 15,000 mg/kg body weight of Rang Chuet leaf extracts prepared by three different extraction solvents, namely water, ethanol, and acetone. The Rang Chuet water extract showed the highest total phenolic contents at 2643 ± 195.05 mg GAE/100 g while the Rang Chuet acetone extract showed the lowest IC₅₀ at 52.91 mg/ml by DPPH assay. The sub-chronic toxicity study was performed using Wistar rats of both sexes which were gavaged with ethanol and water Rang Chuet extracts for 90 days. Rats were gavaged with the equivalent dose of Rang Chuet in a typical consumer drink (to be taken 3 times a day, at dose 1460 mg/kg/day for water extract and 1025 mg/kg/day for ethanol extract), 3000 and 5000 mg/kg. The satellite group was given the same dose of both extracts for 90 days and observed thereafter for 14 days in order to study the reversibility of the adverse effects. The results revealed that none of the Rang Chuet extracts altered the general behavior or mortality or changes in the gross morphology and any histology of the rats’ visceral organs. For sub-chronic toxicity, the result showed that the treatment of ethanol and water Rang Chuet extracts had no significant effect on average body weight, relative organ weights, histopathology of organs, clinical biochemistry, hematological parameters or liver enzymes. This analysis of by-products of a lipid peroxidation study suggested a trend of decreasing malondialdehyde levels in most of the Rang Chuet treated groups. In conclusion, the safety value of Rang Chuet water extract and Rang Chuet ethanol extract in rats is 50 mg/kg body weight which indicate safe dose of Rang Chuet dried powder is 10.27 g/60 kg body weight per day.

Thunbergia laurifolia Linn. (Rang Chuet, RC), a Thai medicinal plant, possesses various bioactive compounds with potential health benefits. This study aimed to identify detoxifying compounds within RC crude extract. RC leaves were extracted using the Soxhlet method with chloroform. Total carotenoids, chlorophylls, extract yield, total phenolic contents (TPCs), and total flavonoid contents (TFCs) were measured. The extract’s composition was analyzed. Cytotoxicity and effects on the detoxification enzyme NQO-1 were assessed in liver cell lines (AML12 and HepG2) using MTT and NQO-1 assays, respectively. Bioactive fractions were identified using fractionation techniques and mass spectrometry (LC-MS). RC extract displayed significant levels of carotenoids (0.375 mg/g), chlorophylls (2.682 mg/g), and favorable yield (15.3%). TPC and TFC were 363.776 mg/g and 112.22 mg/g of extract, respectively. Analysis revealed phenolic acids (gallic acid, caffeic acid), flavonoid (apigenin), chlorophylls (chlorophylls a, b, pheophytin a and b), and lutein. Among the fractions, Fraction 3 (F3) exhibited the highest NQO-1 enzyme activity. F3 contained pheophytin a and hydroxy pheophytin a, confirmed by LC-MS (m/z 871.59⁺ [M + H]⁺ and 887.59⁺ [M + H]⁺). F3 significantly induced NQO-1 activity in both HepG2 (3.908-fold) and AML12 (1.99-fold) cells. This study identified F3 from RC extract as a promising fraction containing pheophytin a and hydroxy pheophytin a, responsible for inducing the detoxification enzyme NQO-1 in liver cells. These findings suggest RC’s potential for promoting detoxification.