This study introduces a new zircon reference material, LKZ-1, for the in situ U–Pb dating and O–Hf isotopic and trace element analyses. The secondary ion mass spectrometric analyses for this gem-quality single-crystal zircon yielded a weighted mean ²⁰⁶Pb/²³⁸U age of 572.6 ± 2.0 Ma (2σ, n = 22, MSWD = 0.90), with moderately high U concentrations (619 ± 21 ppm, 1 SD), restricted Th/U ratios (0.146 ± 0.002, 1 SD), and negligible common Pb content (²⁰⁶Pb_c < 0.2%). A comparable ²⁰⁶Pb/²³⁸U age (570.0 ± 2.5 Ma, 2σ) was produced by the isotope dilution-thermal ionization mass spectrometry. The secondary ion mass spectrometric and laser ablation-assisted multiple collector inductively coupled plasma mass spectrometer analyses respectively showed that LKZ-1 had little variation in O (δ¹⁸O_V-SMOW = 10.65 ± 0.14‰; laser fluorination value = 10.72 ± 0.02‰; 1 SD) and Hf (¹⁷⁶Hf/¹⁷⁷Hf = 0.281794 ± 0.000016, 1 SD) isotopic compositions. LKZ-1 was also fairly homogeneous in its chemical composition (RSD of laser ablation ICPMS data ≤ 10%), displaying a relatively uniform chondrite-normalized rare earth element pattern ((Lu/Gd)_N = 31 ± 3, Eu/Eu^* = 0.43 ± 0.17, Ce/Ce^* = 44 ± 32; 1 SD). These consistencies suggest that the LKZ-1 zircon is a suitable working standard for geochronological and geochemical analyses.