Complete ammonia oxidizer (Comammox) can complete the whole nitrification process independently, whose niche differentiation is important guarantee for its survival and ecological function. This study investigated the niche differentiation of comammox Nitrospira in the sediments of three typical tributaries of the Three Gorges Reservoir (TGR). Clade A and clade B of comammox Nitrospira coexisted in all sampling sites simultaneously. The amoA gene abundance of clade A and B was gradually increased or decreased along the flow path of the three tributaries with obvious spatial differentiation. The amoA gene abundance of comammox Nitrospira clade A (6.36 × 10³ − 5.06 × 10⁴ copies g⁻¹ dry sediment) was higher than that of clade B (6.26 × 10² − 6.27 × 10³ copies g⁻¹ dry sediment), and the clade A amoA gene abundance was one order of magnitude higher than that of AOA (7.24 × 10² − 6.89 × 10³ copies g⁻¹ dry sediment) and AOB (1.44 × 10² − 1.46 × 10³ copies g⁻¹ dry sediment). A significant positive correlation was observed between comammox Nitrospira clade A amoA gene abundance and flow distance (P < 0.05). The number of operational taxonomic units (OTUs) in two sub-clades of clade A accounted for the majority in different tributaries, indicating that clade A also had population differentiation among different tributaries. This study revealed that comammox Nitrospira in the sediments of TGR tributaries have niche differentiation and clade A.2 played a more crucial role in comammox Nitrospira community.

The construction of the reservoir has changed the nitrogen migration and transformation processes in the river, and a large amount of sediment deposition in the reservoir may also lead to the spatial differentiation of complete ammonia oxidation (comammox) bacteria. The study investigated the abundance and diversity of comammox bacteria in the sediments of three cascade reservoirs, namely, Xiaowan, Manwan, and Nuozhadu on the Lancang River in China. In these reservoirs, the average amoA gene abundance of clade A and clade B of comammox bacteria, ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB) was 4.16 ± 0.85 × 10⁵, 1.15 ± 0.33 × 10⁵, 7.39 ± 2.31 × 10⁴, and 3.28 ± 0.99 × 10⁵ copies g^-1, respectively. The abundance of clade A was higher than that of other ammonia oxidizing microorganisms. The spatial variation of comammox bacteria abundance differed among different reservoirs, but the spatial variation trends of the two clades of comammox bacteria in the same reservoir were similar. At each sampling point, clade A1, clade A2, and clade B coexisted, and clade A2 was usually the dominant species. The connection between comammox bacteria in the pre-dam sediments was looser than that in non-pre-dam sediments, and comammox bacteria in pre-dam sediments exhibited a simpler network structure. The main factor affecting comammox bacteria abundance was NH₄⁺-N, while altitude, temperature, and conductivity of overlying water were the main factors affecting comammox bacteria diversity. Environmental changes caused by differences in the spatial distribution of these cascade reservoirs may be the main driver of the changes of community composition and abundance of comammox bacteria. This study confirms that the construction of cascade reservoirs results in niche spatial differentiation of comammox bacteria.

It is necessary to review changes in the interactions of indicators following the construction of the Three Gorges Dam (TGD) in order to explore the impact of the dam on ecology. Research on changes in interactions among indicators of the comprehensive social–economic–ecology system in the Yangtze River Basin is limited, and the objective of this study was to investigate how this system changed after the operation of the TGD, as well as how the indicators contributed to this change. Here, the correlational network approach using 38 data point indicators from 1949 to 2018 of the Yangtze River Basin was applied to analyze the changes in indicator interactions before and after the TGD operation. The TGD impoundment altered networks of the social–economic–ecological system in the Yangtze River Basin. Indicators are both less positively and less negatively connected. The number of synergy and trade-off networks clusters changed from two (Modularity = 0.33) to -six (Modularity = 0.23) and from two (Modularity = 0.015) to four (Modularity = 0.34) after the TGD operation, indicating that the sustainable development of the Yangtze River Basin might be at a middle level after the TGD operation. Further analysis revealed that the mean annual discharge, downstream fry runoff, and downstream counts of the eggs and larvae of four carp, diatom abundance index, breeding population of Chinese sturgeon, and annual precipitation contributed more to the changes in the networks after 2003.