DNA replication is a highly demanding process regarding the energy and material supply and must be precisely regulated, involving multiple cellular feedbacks. The slowing down or stalling of DNA synthesis and/or replication forks is referred to as replication stress (RS). Owing to the complexity and requirements of replication, a plethora of factors may interfere and challenge the genome stability, cell survival or affect the whole organism. This review outlines chemical compounds that are known inducers of RS and commonly used in laboratory research. These compounds act on replication by direct interaction with DNA causing DNA crosslinks and bulky lesions (cisplatin), chemical interference with the metabolism of deoxyribonucleotide triphosphates (hydroxyurea), direct inhibition of the activity of replicative DNA polymerases (aphidicolin) and interference with enzymes dealing with topological DNA stress (camptothecin, etoposide). As a variety of mechanisms can induce RS, the responses of mammalian cells also vary. Here, we review the activity and mechanism of action of these compounds based on recent knowledge, accompanied by examples of induced phenotypes, cellular readouts and commonly used doses.

Quantitative analysis of photo-manipulated samples using a specific setting of the standard laser-scanning microscope is presented. Enclosed software solution is implementing known parameters of the pattern and allows for a quantitative analysis of various laser-induced phenotypes in live or fixed cells. Two software versions based on the same principle are uploaded here, the first one can be used for DNA damage analysis (positive stripes) and the second one for FRAP (negative stripes). The software can be downloaded from: Cells and Stripes Software