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Prof. Zhiming Yuan
Chinese Academy of Sciences

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0 Bioinformatics
0 Biosafety
0 Tropical Diseases
0 Arbovirus
0 entomopathogenic bacteria

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Journal article
Published: 10 August 2021 in Viruses
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Given that ebolavirus causes severe and frequently lethal disease, its rapid and accurate detection using available and validated methods is essential for controlling infection. Real-time reverse-transcription PCR (RT-PCR) has proven to be an invaluable tool for ebolaviruses diagnostics. Many assays with different targets have been developed, but they have not been externally compared or validated, and limits of detection are not uniformly reported. Here we compared and evaluated the sensitivity, reproducibility and specificity of 23 in-house assays under the same conditions. Our results showed that these assays were highly gene- and species- specific when evaluated using in vitro RNA transcripts and viral RNA, and the potential limits of detection were uniformly reported ranging from 102 to 106 in vitro synthesized RNA transcripts copies perμL and 1–100 TCID50/mL. The comparison of these assays indicated that those targeting the more conservative NP gene could be the better option for EVD case definition and quantitative measurement because of its higher sensitivity for the same species. Our analysis could contribute to the standardization of ebolavirus detection and quantification assays, which can offer a better understanding of the meaning of results across laboratories and time points, as well as make them easy to implement, especially under outbreak conditions.

ACS Style

Yi Huang; Shuqi Xiao; Zhiming Yuan. Comparison and Evaluation of Real-Time Taqman PCR for Detection and Quantification of Ebolavirus. Viruses 2021, 13, 1575 .

AMA Style

Yi Huang, Shuqi Xiao, Zhiming Yuan. Comparison and Evaluation of Real-Time Taqman PCR for Detection and Quantification of Ebolavirus. Viruses. 2021; 13 (8):1575.

Chicago/Turabian Style

Yi Huang; Shuqi Xiao; Zhiming Yuan. 2021. "Comparison and Evaluation of Real-Time Taqman PCR for Detection and Quantification of Ebolavirus." Viruses 13, no. 8: 1575.

Journal article
Published: 17 June 2021 in Virus Research
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This study characterized two novel Siphoviridae phages, PW2 and PW4, that can infect 52% and 44% of the tested Bacillus cereus group isolates and display relatively high activity against four cereulide-producing isolates belonging to B. weihenstephanensis and B. paranthracis. The genome sequences of PW2 and PW4 are similar to six known phages infecting B. cereus group isolates, which can be classified into two conserved groups, with the PW2 genome harboring conserved coding sequences (CDSs) from both groups. Two phage-derived endolysins, LysPW2 and LysPW4, which are predicted to encode N-acetylmuramoyl-L-alanine amidase, and their enzymatically active domains (EADs), LysPW2-EAD and LysPW4-EAD, were heterologously expressed. Both LysPW2 and LysPW4, especially the former, show a much wider host range than the phages, albeit still limited to the B. cereus group for the tested bacteria. The optimal temperature and pH for LysPW2 ability is 37 °C and pH 8.0 and for LysPW4 is 50 °C and pH 9.0. Neither LysPW2-EAD nor LysPW4-EAD show any lytic activity against vegetative cells of the tested B. cereus group isolates but can inhibit germination in 66.3% and 65.7% of spores, respectively. In addition, both LysPW2-EAD and LysPW4-EAD exhibit spore-binding capabilities.

ACS Style

Xiaofu Wan; Peiling Geng; Jiahui Sun; Zhiming Yuan; Xiaomin Hu. Characterization of two newly isolated bacteriophages PW2 and PW4 and derived endolysins with lysis activity against Bacillus cereus group strains. Virus Research 2021, 302, 198489 .

AMA Style

Xiaofu Wan, Peiling Geng, Jiahui Sun, Zhiming Yuan, Xiaomin Hu. Characterization of two newly isolated bacteriophages PW2 and PW4 and derived endolysins with lysis activity against Bacillus cereus group strains. Virus Research. 2021; 302 ():198489.

Chicago/Turabian Style

Xiaofu Wan; Peiling Geng; Jiahui Sun; Zhiming Yuan; Xiaomin Hu. 2021. "Characterization of two newly isolated bacteriophages PW2 and PW4 and derived endolysins with lysis activity against Bacillus cereus group strains." Virus Research 302, no. : 198489.

Review article
Published: 01 June 2021 in Journal of Biosafety and Biosecurity
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The Biosecurity Law of the People’s Republic of China is the first basic, comprehensive, systematic, and overarching law of biosecurity in China, involving biosecurity risks, national biosecurity governance, and systems for minimizing the biosecurity threats. This article elaborates on the formulation, content, features, and significance of the law, and provides an outlook for its evolution.

ACS Style

Liang Huigang; Huang Cui; Zhu Xiaoli; Yuan Zhiming. Significance of and outlook for the Biosecurity Law of the People’s Republic of China. Journal of Biosafety and Biosecurity 2021, 3, 46 -50.

AMA Style

Liang Huigang, Huang Cui, Zhu Xiaoli, Yuan Zhiming. Significance of and outlook for the Biosecurity Law of the People’s Republic of China. Journal of Biosafety and Biosecurity. 2021; 3 (1):46-50.

Chicago/Turabian Style

Liang Huigang; Huang Cui; Zhu Xiaoli; Yuan Zhiming. 2021. "Significance of and outlook for the Biosecurity Law of the People’s Republic of China." Journal of Biosafety and Biosecurity 3, no. 1: 46-50.

Journal article
Published: 11 May 2021 in Nature Communications
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COVID-19 pandemic caused by SARS-CoV-2 constitutes a global public health crisis with enormous economic consequences. Monoclonal antibodies against SARS-CoV-2 can provide an important treatment option to fight COVID-19, especially for the most vulnerable populations. In this work, potent antibodies binding to SARS-CoV-2 Spike protein were identified from COVID-19 convalescent patients. Among them, P4A1 interacts directly with and covers majority of the Receptor Binding Motif of the Spike Receptor-Binding Domain, shown by high-resolution complex structure analysis. We further demonstrate the binding and neutralizing activities of P4A1 against wild type and mutant Spike proteins or pseudoviruses. P4A1 was subsequently engineered to reduce the potential risk for Antibody-Dependent Enhancement of infection and to extend its half-life. The engineered antibody exhibits an optimized pharmacokinetic and safety profile, and it results in complete viral clearance in a rhesus monkey model of COVID-19 following a single injection. These data suggest its potential against SARS-CoV-2 related diseases.

ACS Style

Yu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Qian Zhang; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yarong Li; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander G. Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhiming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. Nature Communications 2021, 12, 1 -11.

AMA Style

Yu Guo, Lisu Huang, Guangshun Zhang, Yanfeng Yao, He Zhou, Shu Shen, Bingqing Shen, Bo Li, Xin Li, Qian Zhang, Mingjie Chen, Da Chen, Jia Wu, Dan Fu, Xinxin Zeng, Mingfang Feng, ChunJiang Pi, Yuan Wang, Xingdong Zhou, Minmin Lu, Yarong Li, Yaohui Fang, Yun-Yueh Lu, Xue Hu, Shanshan Wang, Wanju Zhang, Ge Gao, Francisco Adrian, Qisheng Wang, Feng Yu, Yun Peng, Alexander G. Gabibov, Juan Min, Yuhui Wang, Heyu Huang, Alexey Stepanov, Wei Zhang, Yan Cai, Junwei Liu, Zhiming Yuan, Chen Zhang, Zhiyong Lou, Fei Deng, Hongkai Zhang, Chao Shan, Liang Schweizer, Kun Sun, Zihe Rao. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. Nature Communications. 2021; 12 (1):1-11.

Chicago/Turabian Style

Yu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Qian Zhang; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yarong Li; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander G. Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhiming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. 2021. "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes." Nature Communications 12, no. 1: 1-11.

Research article
Published: 09 April 2021 in Virologica Sinica
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The ongoing coronavirus disease 2019 (COVID-19) pandemic caused more than 96 million infections and over 2 million deaths worldwide so far. However, there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the disease causative agent. Vaccine is the most effective approach to eradicate a pathogen. The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations. Here we evaluated the safety, immunogenicity, and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates. Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates, and subsequently provided partial (in low dose) or full (in high dose) protection of challenge in the tested animals. In addition, passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice. These results warranted positive outcomes in future clinical trials in humans.

ACS Style

Yan-Feng Yao; Ze-Jun Wang; Ren-Di Jiang; Xue Hu; Hua-Jun Zhang; Yi-Wu Zhou; Ge Gao; Ying Chen; Yun Peng; Mei-Qin Liu; Ya-Nan Zhang; Juan Min; Jia Lu; Xiao-Xiao Gao; Jing Guo; Cheng Peng; Xu-Rui Shen; Qian Li; Kai Zhao; Lian Yang; Xin Wan; Bo Zhang; Wen-Hui Wang; Jia Wu; Peng Zhou; Xing-Lou Yang; Shuo Shen; Chao Shan; Zhi-Ming Yuan; Zheng-Li Shi. Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates. Virologica Sinica 2021, 1 -11.

AMA Style

Yan-Feng Yao, Ze-Jun Wang, Ren-Di Jiang, Xue Hu, Hua-Jun Zhang, Yi-Wu Zhou, Ge Gao, Ying Chen, Yun Peng, Mei-Qin Liu, Ya-Nan Zhang, Juan Min, Jia Lu, Xiao-Xiao Gao, Jing Guo, Cheng Peng, Xu-Rui Shen, Qian Li, Kai Zhao, Lian Yang, Xin Wan, Bo Zhang, Wen-Hui Wang, Jia Wu, Peng Zhou, Xing-Lou Yang, Shuo Shen, Chao Shan, Zhi-Ming Yuan, Zheng-Li Shi. Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates. Virologica Sinica. 2021; ():1-11.

Chicago/Turabian Style

Yan-Feng Yao; Ze-Jun Wang; Ren-Di Jiang; Xue Hu; Hua-Jun Zhang; Yi-Wu Zhou; Ge Gao; Ying Chen; Yun Peng; Mei-Qin Liu; Ya-Nan Zhang; Juan Min; Jia Lu; Xiao-Xiao Gao; Jing Guo; Cheng Peng; Xu-Rui Shen; Qian Li; Kai Zhao; Lian Yang; Xin Wan; Bo Zhang; Wen-Hui Wang; Jia Wu; Peng Zhou; Xing-Lou Yang; Shuo Shen; Chao Shan; Zhi-Ming Yuan; Zheng-Li Shi. 2021. "Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates." Virologica Sinica , no. : 1-11.

Reports
Published: 01 January 2021 in mAbs
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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which causes coronavirus disease-2019 (COVID-19), interacts with the host cell receptor angiotensin-converting enzyme 2 (hACE2) via its spike 1 protein during infection. After the virus sequence was published, we identified two potent antibodies against the SARS-CoV-2 receptor binding domain (RBD) from antibody libraries using a phage-to-yeast (PtY) display platform in only 10 days. Our lead antibody JMB2002, now in a Phase 1 clinical trial (ChiCTR2100042150), showed broad-spectrum in vitro blocking activity against hACE2 binding to the RBD of multiple SARS-CoV-2 variants, including B.1.351 that was reportedly much more resistant to neutralization by convalescent plasma, vaccine sera and some clinical-stage neutralizing antibodies. Furthermore, JMB2002 has demonstrated complete prophylactic and potent therapeutic efficacy in a rhesus macaque disease model. Prophylactic and therapeutic countermeasure intervention of SARS-CoV-2 using JMB2002 would likely slow down the transmission of currently emerged SARS-CoV-2 variants and result in more efficient control of the COVID-19 pandemic.

ACS Style

Chunyin Gu; Xiaodan Cao; Zongda Wang; Xue Hu; Yanfeng Yao; Yiwu Zhou; Peipei Liu; Xiaowu Liu; Ge Gao; Xiao Hu; Yecheng Zhang; Zhen Chen; Li Gao; Yun Peng; Fangfang Jia; Chao Shan; Li Yu; Kunpeng Liu; Nan Li; Weiwei Guo; Guoping Jiang; Juan Min; Jianjian Zhang; Lu Yang; Meng Shi; Tianquan Hou; Yanan Li; Weichen Liang; Guoqiao Lu; Congyi Yang; Yuting Wang; Kaiwen Xia; Zheng Xiao; Jianhua Xue; Xueyi Huang; Xin Chen; Haixia Ma; Donglin Song; ZhongZong Pan; Xueping Wang; Haibing Guo; Hong Liang; Zhiming Yuan; Wuxiang Guan; Su-Jun Deng. A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351. mAbs 2021, 13, 1930636 .

AMA Style

Chunyin Gu, Xiaodan Cao, Zongda Wang, Xue Hu, Yanfeng Yao, Yiwu Zhou, Peipei Liu, Xiaowu Liu, Ge Gao, Xiao Hu, Yecheng Zhang, Zhen Chen, Li Gao, Yun Peng, Fangfang Jia, Chao Shan, Li Yu, Kunpeng Liu, Nan Li, Weiwei Guo, Guoping Jiang, Juan Min, Jianjian Zhang, Lu Yang, Meng Shi, Tianquan Hou, Yanan Li, Weichen Liang, Guoqiao Lu, Congyi Yang, Yuting Wang, Kaiwen Xia, Zheng Xiao, Jianhua Xue, Xueyi Huang, Xin Chen, Haixia Ma, Donglin Song, ZhongZong Pan, Xueping Wang, Haibing Guo, Hong Liang, Zhiming Yuan, Wuxiang Guan, Su-Jun Deng. A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351. mAbs. 2021; 13 (1):1930636.

Chicago/Turabian Style

Chunyin Gu; Xiaodan Cao; Zongda Wang; Xue Hu; Yanfeng Yao; Yiwu Zhou; Peipei Liu; Xiaowu Liu; Ge Gao; Xiao Hu; Yecheng Zhang; Zhen Chen; Li Gao; Yun Peng; Fangfang Jia; Chao Shan; Li Yu; Kunpeng Liu; Nan Li; Weiwei Guo; Guoping Jiang; Juan Min; Jianjian Zhang; Lu Yang; Meng Shi; Tianquan Hou; Yanan Li; Weichen Liang; Guoqiao Lu; Congyi Yang; Yuting Wang; Kaiwen Xia; Zheng Xiao; Jianhua Xue; Xueyi Huang; Xin Chen; Haixia Ma; Donglin Song; ZhongZong Pan; Xueping Wang; Haibing Guo; Hong Liang; Zhiming Yuan; Wuxiang Guan; Su-Jun Deng. 2021. "A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351." mAbs 13, no. 1: 1930636.

Research article
Published: 30 November 2020 in PLOS Neglected Tropical Diseases
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Advances in technology have greatly stimulated the understanding of insect-specific viruses (ISVs). Unfortunately, most of these findings are based on sequencing technology, and laboratory data are scarce on the transmission dynamics of ISVs in nature and the potential effects of these viruses on arboviruses. Mesonivirus is a class of ISVs with a wide geographical distribution. Recently, our laboratory reported the isolation of a novel strain of mesonivirus, Yichang virus (YCV), from Culex mosquitoes, China. In this study, the experimental infection of YCV by the oral route for adult and larvae mosquitoes, and the vertical transmission has been conducted, which suggests that YCV could adopt a mixed-mode transmission. Controlled experiments showed that the infectivity of YCV depends on the mosquito species, virus dose, and infection route. The proliferation curve and tissue distribution of YCV in Cx. quinquefasciatus and Ae. albopictus showed that YCV is more susceptible to Ae. albopictus and is located in the midgut. Furthermore, we also assessed the interference of YCV with flaviviruses both in vitro and in vivo. YCV significantly inhibited the proliferation of DENV-2 and ZIKV, in cell culture, and reduced transmission rate of DENV-2 in Ae. albopictus. Our work provides insights into the transmission of ISVs in different mosquito species during ontogeny and their potential ability to interact with mosquito-borne viruses.

ACS Style

Guoguo Ye; Yujuan Wang; Xiaoyun Liu; Qiannan Dong; Quanxing Cai; Zhiming Yuan; Han Xia. Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. PLOS Neglected Tropical Diseases 2020, 14, e0008920 .

AMA Style

Guoguo Ye, Yujuan Wang, Xiaoyun Liu, Qiannan Dong, Quanxing Cai, Zhiming Yuan, Han Xia. Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. PLOS Neglected Tropical Diseases. 2020; 14 (11):e0008920.

Chicago/Turabian Style

Guoguo Ye; Yujuan Wang; Xiaoyun Liu; Qiannan Dong; Quanxing Cai; Zhiming Yuan; Han Xia. 2020. "Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses." PLOS Neglected Tropical Diseases 14, no. 11: e0008920.

Review
Published: 14 November 2020 in Pathogens and Global Health
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Zika virus (ZIKV) is an emerging arthropod-borne flavivirus that, upon infection, results in teratogenic effects and neurological disorders. ZIKV infections pose serious global public health concerns, prompting scientists to increase research on antivirals and vaccines against the virus. These efforts are still ongoing as the pathogenesis and immune evasion mechanisms of ZIKV have not yet been fully elaborated. Currently, no specific vaccines or drugs have been approved for ZIKV; however, some are undergoing clinical trials. Notably, several strategies have been used to develop antivirals, including drugs that target viral and host proteins. Additionally, drug repurposing is preferred since it is less costly and takes less time than other strategies because the drugs used have already been approved for human use. Likewise, different platforms have been evaluated for the design of vaccines, including DNA, mRNA, peptide, protein, viral vectors, virus-like particles (VLPSs), inactivated-virus, and live-attenuated virus vaccines. These vaccines have been shown to induce specific humoral and cellular immune responses and reduce viremia and viral RNA both in vitro and in vivo. Importantly, most of these vaccines have entered clinical trials. Understanding the viral disease mechanism will provide better strategies for developing therapeutic agents against ZIKV. This review provides a comprehensive summary of the viral pathogenesis of ZIKV and current advancements in the development of vaccines and drugs against this virus. Graphical abstract

ACS Style

Caroline Mwaliko; Raphael Nyaruaba; Lu Zhao; Evans Atoni; Samuel Karungu; Matilu Mwau; Dimitri Lavillette; Han Xia; Zhiming Yuan. Zika virus pathogenesis and current therapeutic advances. Pathogens and Global Health 2020, 115, 21 -39.

AMA Style

Caroline Mwaliko, Raphael Nyaruaba, Lu Zhao, Evans Atoni, Samuel Karungu, Matilu Mwau, Dimitri Lavillette, Han Xia, Zhiming Yuan. Zika virus pathogenesis and current therapeutic advances. Pathogens and Global Health. 2020; 115 (1):21-39.

Chicago/Turabian Style

Caroline Mwaliko; Raphael Nyaruaba; Lu Zhao; Evans Atoni; Samuel Karungu; Matilu Mwau; Dimitri Lavillette; Han Xia; Zhiming Yuan. 2020. "Zika virus pathogenesis and current therapeutic advances." Pathogens and Global Health 115, no. 1: 21-39.

Preprint content
Published: 14 November 2020
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The Coronavirus Disease of 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) threatens global public health and economy. Therapeutic options such as monoclonal antibodies (mAbs) against SARS-CoV-2 are in urgent need. We have identified potent monoclonal antibodies binding to SARS-CoV-2 Spike protein from COVID-19 convalescent patients and one of these antibodies, P4A1, interacts directly and covers the majority of the Receptor Binding Motif (RBM) of Spike receptor-binding domain (RBD), shown by high-resolution complex structure analysis. We further demonstrated P4A1 binding and neutralizing activities against wild type and mutant spike proteins. P4A1 was subsequently engineered to reduce the potential risk for antibody-dependent enhancement (ADE) of infection and to extend its half-life. The engineered mAb exhibits optimized pharmacokinetic and safety profile, and results in complete viral clearance in a rhesus monkey model of COVID-19 following a single injection.

ACS Style

Yu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Qian Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhi-Ming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. A SARS-CoV-2 neutralizing antibody with exceptional spike binding coverage and optimized therapeutic potentials. 2020, 1 .

AMA Style

Yu Guo, Lisu Huang, Guangshun Zhang, Yanfeng Yao, He Zhou, Shu Shen, Bingqing Shen, Bo Li, Xin Li, Mingjie Chen, Da Chen, Jia Wu, Dan Fu, Xinxin Zeng, Mingfang Feng, ChunJiang Pi, Yuan Wang, Xingdong Zhou, Minmin Lu, Yaohui Fang, Yun-Yueh Lu, Xue Hu, Shanshan Wang, Wanju Zhang, Qian Zhang, Ge Gao, Francisco Adrian, Qisheng Wang, Feng Yu, Yun Peng, Alexander Gabibov, Juan Min, Yuhui Wang, Heyu Huang, Alexey Stepanov, Wei Zhang, Yan Cai, Junwei Liu, Zhi-Ming Yuan, Chen Zhang, Zhiyong Lou, Fei Deng, Hongkai Zhang, Chao Shan, Liang Schweizer, Kun Sun, Zihe Rao. A SARS-CoV-2 neutralizing antibody with exceptional spike binding coverage and optimized therapeutic potentials. . 2020; ():1.

Chicago/Turabian Style

Yu Guo; Lisu Huang; Guangshun Zhang; Yanfeng Yao; He Zhou; Shu Shen; Bingqing Shen; Bo Li; Xin Li; Mingjie Chen; Da Chen; Jia Wu; Dan Fu; Xinxin Zeng; Mingfang Feng; ChunJiang Pi; Yuan Wang; Xingdong Zhou; Minmin Lu; Yaohui Fang; Yun-Yueh Lu; Xue Hu; Shanshan Wang; Wanju Zhang; Qian Zhang; Ge Gao; Francisco Adrian; Qisheng Wang; Feng Yu; Yun Peng; Alexander Gabibov; Juan Min; Yuhui Wang; Heyu Huang; Alexey Stepanov; Wei Zhang; Yan Cai; Junwei Liu; Zhi-Ming Yuan; Chen Zhang; Zhiyong Lou; Fei Deng; Hongkai Zhang; Chao Shan; Liang Schweizer; Kun Sun; Zihe Rao. 2020. "A SARS-CoV-2 neutralizing antibody with exceptional spike binding coverage and optimized therapeutic potentials." , no. : 1.

Journal article
Published: 13 November 2020 in Nature Communications
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Efficacious interventions are urgently needed for the treatment of COVID-19. Here, we report a monoclonal antibody (mAb), MW05, with SARS-CoV-2 neutralizing activity by disrupting the interaction of receptor binding domain (RBD) with angiotensin-converting enzyme 2 (ACE2) receptor. Crosslinking of Fc with FcγRIIB mediates antibody-dependent enhancement (ADE) activity by MW05. This activity is eliminated by introducing the LALA mutation to the Fc region (MW05/LALA). Potent prophylactic and therapeutic effects against SARS-CoV-2 are observed in rhesus monkeys. A single dose of MW05/LALA blocks infection of SARS-CoV-2 in prophylactic treatment and clears SARS-CoV-2 in three days in a therapeutic treatment setting. These results pave the way for the development of MW05/LALA as an antiviral strategy for COVID-19.

ACS Style

Shuang Wang; Yun Peng; Rongjuan Wang; Shasha Jiao; Min Wang; Weijin Huang; Chao Shan; Wen Jiang; Zepeng Li; Chunying Gu; Ben Chen; Xue Hu; Yanfeng Yao; Juan Min; Huajun Zhang; Ying Chen; Ge Gao; Peipei Tang; Gang Li; An Wang; Jinchao Zhang; Shuo Chen; Xun Gui; Zhiming Yuan; Datao Liu. Characterization of neutralizing antibody with prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus monkeys. Nature Communications 2020, 11, 1 -8.

AMA Style

Shuang Wang, Yun Peng, Rongjuan Wang, Shasha Jiao, Min Wang, Weijin Huang, Chao Shan, Wen Jiang, Zepeng Li, Chunying Gu, Ben Chen, Xue Hu, Yanfeng Yao, Juan Min, Huajun Zhang, Ying Chen, Ge Gao, Peipei Tang, Gang Li, An Wang, Jinchao Zhang, Shuo Chen, Xun Gui, Zhiming Yuan, Datao Liu. Characterization of neutralizing antibody with prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus monkeys. Nature Communications. 2020; 11 (1):1-8.

Chicago/Turabian Style

Shuang Wang; Yun Peng; Rongjuan Wang; Shasha Jiao; Min Wang; Weijin Huang; Chao Shan; Wen Jiang; Zepeng Li; Chunying Gu; Ben Chen; Xue Hu; Yanfeng Yao; Juan Min; Huajun Zhang; Ying Chen; Ge Gao; Peipei Tang; Gang Li; An Wang; Jinchao Zhang; Shuo Chen; Xun Gui; Zhiming Yuan; Datao Liu. 2020. "Characterization of neutralizing antibody with prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus monkeys." Nature Communications 11, no. 1: 1-8.

Author correction
Published: 04 November 2020 in Scientific Data
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An amendment to this paper has been published and can be accessed via a link at the top of the paper.

ACS Style

Evans Atoni; Lu Zhao; Cheng Hu; Nanjie Ren; Xiaoyu Wang; Mengying Liang; Caroline Mwaliko; Zhiming Yuan; Han Xia. Author Correction: A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China. Scientific Data 2020, 7, 1 -1.

AMA Style

Evans Atoni, Lu Zhao, Cheng Hu, Nanjie Ren, Xiaoyu Wang, Mengying Liang, Caroline Mwaliko, Zhiming Yuan, Han Xia. Author Correction: A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China. Scientific Data. 2020; 7 (1):1-1.

Chicago/Turabian Style

Evans Atoni; Lu Zhao; Cheng Hu; Nanjie Ren; Xiaoyu Wang; Mengying Liang; Caroline Mwaliko; Zhiming Yuan; Han Xia. 2020. "Author Correction: A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China." Scientific Data 7, no. 1: 1-1.

Data descriptor
Published: 13 October 2020 in Scientific Data
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Mosquito-borne viruses such as Zika virus, Japanese Encephalitis virus and Dengue virus present an increasing global health concern. However, in-depth knowledge of the distribution and diversity of mosquito-associated viruses and their related vectors remains limited, especially for China. To promote their understanding, we present the first comprehensive dataset of the distribution and diversity of these viruses and their related vectors in China (including Taiwan, Hong Kong and Macau). Data was drawn from peer-reviewed journal articles, conference papers and thesis publications in both English and Chinese. Geographical data on mosquito-associated viruses’ occurrence and related mosquito vector species was extracted, and quality-control processes employed. This dataset contains 2,428 accounts of mosquito-associated viruses’ and mosquito species geo-referenced occurrences at various administrative levels in China. The prevalent mosquito-associated virus includes Japanese encephalitis virus, Dengue virus, Banna virus and Culex flavivirus, whereas the abundant mosquito vectors are Culex tritaeryohynchus, Aedes albopictus and Culex pipiens pallens. This geographical dataset delivers a distribution and diversity outline of mosquito-associated viruses in China, and also applicable in various spatial and risk-assessment analysis.

ACS Style

Evans Atoni; Lu Zhao; Cheng Hu; Nanjie Ren; Xiaoyu Wang; Mengying Liang; Caroline Mwaliko; Zhiming Yuan; Han Xia. A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China. Scientific Data 2020, 7, 1 -7.

AMA Style

Evans Atoni, Lu Zhao, Cheng Hu, Nanjie Ren, Xiaoyu Wang, Mengying Liang, Caroline Mwaliko, Zhiming Yuan, Han Xia. A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China. Scientific Data. 2020; 7 (1):1-7.

Chicago/Turabian Style

Evans Atoni; Lu Zhao; Cheng Hu; Nanjie Ren; Xiaoyu Wang; Mengying Liang; Caroline Mwaliko; Zhiming Yuan; Han Xia. 2020. "A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China." Scientific Data 7, no. 1: 1-7.

Letter to the editor
Published: 25 August 2020 in Cell Research
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Zhou, P. et al. Nature 579, 270–273 (2020). CAS Article Google Scholar Sun, S. H. et al. Cell Host Microbe 28, 124–133 (2020). CAS Article Google Scholar Bao, L. et al. Nature 583, 830–833 (2020). CAS Article Google Scholar Jiang, R. D. et al. Cell 182, 50–58 (2020). CAS Article Google Scholar Sun, J. et al. Cell 182, 734–743 (2020). CAS Article Google Scholar Gu, H. et al. Science https://doi.org/10.1126/science.abc4730 (2020). Article PubMed PubMed Central Google Scholar Wang, J. et al. Protein Cell https://doi.org/10.1007/s13238-020-00767-x (2020). Article PubMed PubMed Central Google Scholar Dinnon, K. H. et al. bioRxiv https://doi.org/10.1101/2020.05.06.081497 (2020). Article PubMed PubMed Central Google Scholar Polo, J. M. et al. Proc. Natl. Acad. Sci. USA 96, 4598–4603 (1999). CAS Article Google Scholar Perri, S. et al. J. Virol. 77, 10394–10403 (2003). CAS Article Google Scholar Frolov, I. et al. Proc. Natl. Acad. Sci. USA 93, 11371–11377 (1996). CAS Article Google Scholar Shi, R. et al. Nature 584, 120–124 (2020). CAS Article Google Scholar Download references This work was supported by the National Key Research and Development Program of China (2018YFA0507201) and the National Natural Science Foundation of China (81702005). The experiments related to SARS-CoV-2 were completed at National Biosafety Laboratory, Wuhan, Chinese Academy of Sciences. We are particularly grateful to Tao Du and Lun Wang from Zhengdian Biosafety Level 3 Laboratory and the running team of the laboratory for their work. These authors contributed equally: Ya-Nan Zhang, Xiao-Dan Li, Zhe-Rui Zhang Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China Ya-Nan Zhang, Xiao-Dan Li, Zhe-Rui Zhang, Hong-Qing Zhang, Na Li, Jing Liu, Jia-Qi Li, Hua-Jun Zhang, Zheng-Li Shi, Hong-Ping Wei, Zhi-Ming Yuan, Han-Qing Ye & Bo Zhang Hunan Normal University, School of Medicine, Changsha, Hunan, 410081, China Xiao-Dan Li Wuhan Institute of Biological Products Co. Ltd., No. 1 Huangjin Industrial Park Road, Jiangxia District, Wuhan, Hubei, 420115, China Ze-Jun Wang & Shuo Shen You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar B.Z., and H.-Q.Y. conceived and designed the study; Y.-N.Z., X.-D.L., Z.-R.Z., H.-Q.Z., N.L., J.L., and J.-Q.L. performed the experiments; Y.-N.Z. and X.-D.L. collected and processed the data; H.-J.Z., Z.-L.S., Z.-J.W., S.S., H.-P.W., and Z.-M.Y. contributed materials and reagents for the experiments; B.Z., H.-Q.Y., Y.-N.Z., and X.-D.L. analyzed the data and wrote the manuscript. All the authors have read the manuscript and provided useful comments. Correspondence to Han-Qing Ye or Bo Zhang. The authors declare no competing interests. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Reprints and Permissions Zhang, Y., Li, X., Zhang, Z. et al. A mouse model for SARS-CoV-2 infection by exogenous delivery of hACE2 using alphavirus replicon particles. Cell Res (2020). https://doi.org/10.1038/s41422-020-00405-5 Download citation Received: 17 July 2020 Accepted: 14 August 2020 Published: 25 August 2020 DOI: https://doi.org/10.1038/s41422-020-00405-5

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Ya-Nan Zhang; Xiao-Dan Li; Zhe-Rui Zhang; Hong-Qing Zhang; Na Li; Jing Liu; Jia-Qi Li; Hua-Jun Zhang; Ze-Jun Wang; Shuo Shen; Zheng-Li Shi; Hong-Ping Wei; Zhi-Ming Yuan; Han-Qing Ye; Bo Zhang. A mouse model for SARS-CoV-2 infection by exogenous delivery of hACE2 using alphavirus replicon particles. Cell Research 2020, 30, 1046 -1048.

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Ya-Nan Zhang, Xiao-Dan Li, Zhe-Rui Zhang, Hong-Qing Zhang, Na Li, Jing Liu, Jia-Qi Li, Hua-Jun Zhang, Ze-Jun Wang, Shuo Shen, Zheng-Li Shi, Hong-Ping Wei, Zhi-Ming Yuan, Han-Qing Ye, Bo Zhang. A mouse model for SARS-CoV-2 infection by exogenous delivery of hACE2 using alphavirus replicon particles. Cell Research. 2020; 30 (11):1046-1048.

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Ya-Nan Zhang; Xiao-Dan Li; Zhe-Rui Zhang; Hong-Qing Zhang; Na Li; Jing Liu; Jia-Qi Li; Hua-Jun Zhang; Ze-Jun Wang; Shuo Shen; Zheng-Li Shi; Hong-Ping Wei; Zhi-Ming Yuan; Han-Qing Ye; Bo Zhang. 2020. "A mouse model for SARS-CoV-2 infection by exogenous delivery of hACE2 using alphavirus replicon particles." Cell Research 30, no. 11: 1046-1048.

Journal article
Published: 21 August 2020 in Nature Communications
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The rapid spread of coronavirus SARS-CoV-2 greatly threatens global public health but no prophylactic vaccine is available. Here, we report the generation of a replication-incompetent recombinant serotype 5 adenovirus, Ad5-S-nb2, carrying a codon-optimized gene encoding Spike protein (S). In mice and rhesus macaques, intramuscular injection with Ad5-S-nb2 elicits systemic S-specific antibody and cell-mediated immune (CMI) responses. Intranasal inoculation elicits both systemic and pulmonary antibody responses but weaker CMI response. At 30 days after a single vaccination with Ad5-S-nb2 either intramuscularly or intranasally, macaques are protected against SARS-CoV-2 challenge. A subsequent challenge reveals that macaques vaccinated with a 10-fold lower vaccine dosage (1 × 1010 viral particles) are also protected, demonstrating the effectiveness of Ad5-S-nb2 and the possibility of offering more vaccine dosages within a shorter timeframe. Thus, Ad5-S-nb2 is a promising candidate vaccine and warrants further clinical evaluation.

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Liqiang Feng; Qian Wang; Chao Shan; Chenchen Yang; Ying Feng; Jia Wu; Xiaolin Liu; Yiwu Zhou; Rendi Jiang; Peiyu Hu; Xinglong Liu; Fan Zhang; Pingchao Li; Xuefeng Niu; Yichu Liu; Xuehua Zheng; Jia Luo; Jing Sun; Yingying Gu; Bo Liu; Yongcun Xu; Chufang Li; Weiqi Pan; Jincun Zhao; Changwen Ke; Xinwen Chen; Tao Xu; Nanshan Zhong; Suhua Guan; Zhiming Yuan; Ling Chen. An adenovirus-vectored COVID-19 vaccine confers protection from SARS-COV-2 challenge in rhesus macaques. Nature Communications 2020, 11, 1 -11.

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Liqiang Feng, Qian Wang, Chao Shan, Chenchen Yang, Ying Feng, Jia Wu, Xiaolin Liu, Yiwu Zhou, Rendi Jiang, Peiyu Hu, Xinglong Liu, Fan Zhang, Pingchao Li, Xuefeng Niu, Yichu Liu, Xuehua Zheng, Jia Luo, Jing Sun, Yingying Gu, Bo Liu, Yongcun Xu, Chufang Li, Weiqi Pan, Jincun Zhao, Changwen Ke, Xinwen Chen, Tao Xu, Nanshan Zhong, Suhua Guan, Zhiming Yuan, Ling Chen. An adenovirus-vectored COVID-19 vaccine confers protection from SARS-COV-2 challenge in rhesus macaques. Nature Communications. 2020; 11 (1):1-11.

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Liqiang Feng; Qian Wang; Chao Shan; Chenchen Yang; Ying Feng; Jia Wu; Xiaolin Liu; Yiwu Zhou; Rendi Jiang; Peiyu Hu; Xinglong Liu; Fan Zhang; Pingchao Li; Xuefeng Niu; Yichu Liu; Xuehua Zheng; Jia Luo; Jing Sun; Yingying Gu; Bo Liu; Yongcun Xu; Chufang Li; Weiqi Pan; Jincun Zhao; Changwen Ke; Xinwen Chen; Tao Xu; Nanshan Zhong; Suhua Guan; Zhiming Yuan; Ling Chen. 2020. "An adenovirus-vectored COVID-19 vaccine confers protection from SARS-COV-2 challenge in rhesus macaques." Nature Communications 11, no. 1: 1-11.

Journal article
Published: 07 July 2020 in Cell Research
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The 2019 novel coronavirus (SARS-CoV-2) outbreak is a major challenge for public health. SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases, with a mortality rate of ~6.4% worldwide (based on World Health Organization daily situation report). However, the dynamics of viral infection, replication and shedding are poorly understood. Here, we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2. After intratracheal inoculation, the first peak of viral RNA was observed in oropharyngeal swabs one day post infection (1 d.p.i.), mainly from the input of the inoculation, while the second peak occurred at 5 d.p.i., which reflected on-site replication in the respiratory tract. Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals, characterized by hyperemia and edema, and infiltration of monocytes and lymphocytes in alveoli. We also identified SARS-CoV-2 RNA in respiratory tract tissues, including trachea, bronchus and lung; and viruses were also re-isolated from oropharyngeal swabs, bronchus and lung, respectively. Furthermore, we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2. The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics.

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Chao Shan; Yan-Feng Yao; Xing-Lou Yang; Yi-Wu Zhou; Ge Gao; Yun Peng; Lian Yang; Xue Hu; Jin Xiong; Ren-Di Jiang; Hua-Jun Zhang; Xiao-Xiao Gao; Cheng Peng; Juan Min; Ying Chen; Hao-Rui Si; Jia Wu; Peng Zhou; Yan-Yi Wang; Hong-Ping Wei; Wei Pang; Zheng-Fei Hu; Long-Bao Lv; Yong-Tang Zheng; Zheng-Li Shi; Zhi-Ming Yuan. Infection with novel coronavirus (SARS-CoV-2) causes pneumonia in Rhesus macaques. Cell Research 2020, 30, 670 -677.

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Chao Shan, Yan-Feng Yao, Xing-Lou Yang, Yi-Wu Zhou, Ge Gao, Yun Peng, Lian Yang, Xue Hu, Jin Xiong, Ren-Di Jiang, Hua-Jun Zhang, Xiao-Xiao Gao, Cheng Peng, Juan Min, Ying Chen, Hao-Rui Si, Jia Wu, Peng Zhou, Yan-Yi Wang, Hong-Ping Wei, Wei Pang, Zheng-Fei Hu, Long-Bao Lv, Yong-Tang Zheng, Zheng-Li Shi, Zhi-Ming Yuan. Infection with novel coronavirus (SARS-CoV-2) causes pneumonia in Rhesus macaques. Cell Research. 2020; 30 (8):670-677.

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Chao Shan; Yan-Feng Yao; Xing-Lou Yang; Yi-Wu Zhou; Ge Gao; Yun Peng; Lian Yang; Xue Hu; Jin Xiong; Ren-Di Jiang; Hua-Jun Zhang; Xiao-Xiao Gao; Cheng Peng; Juan Min; Ying Chen; Hao-Rui Si; Jia Wu; Peng Zhou; Yan-Yi Wang; Hong-Ping Wei; Wei Pang; Zheng-Fei Hu; Long-Bao Lv; Yong-Tang Zheng; Zheng-Li Shi; Zhi-Ming Yuan. 2020. "Infection with novel coronavirus (SARS-CoV-2) causes pneumonia in Rhesus macaques." Cell Research 30, no. 8: 670-677.

Letter
Published: 29 June 2020 in Virologica Sinica
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Chan JF-W, Yuan S, Kok K-H, To KK-W, Chu H, Yang J, Xing F, Liu J, Yip CC-Y, Poon RW-S, Tsoi H-W, Lo SK-F, Chan K-H, Poon VK-M, Chan W-M, Ip JD, Cai J-P, Cheng VC-C, Chen H, Hui CK-M, Yuen K-Y (2020) A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. The Lancet 395:514–523 CAS Article Google Scholar Donoghue M, Hsieh F, Baronas E, Godbout K, Gosselin M, Stagliano N, Donovan M, Woolf B, Robison K, Jeyaseelan R, Breitbart RE, Acton S (2000) A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Circ Res 87:E1–E9 CAS Article Google Scholar Ekbote U, Coates D, Isaac RE (1999) A mosquito (Anopheles stephensi) angiotensin I-converting enzyme (ACE) is induced by a blood meal and accumulates in the developing ovary. FEBS Lett 455:219–222 CAS Article Google Scholar Franz AW, Kantor AM, Passarelli AL, Clem RJ (2015) Tissue barriers to arbovirus infection in mosquitoes. Viruses 7:3741–3767 CAS Article Google Scholar Hamming I, Timens W, Bulthuis ML, Lely AT, Navis G, van Goor H (2004) Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol 203:631–637 CAS Article Google Scholar Huang YS, Higgs S, Vanlandingham DL (2019) Arbovirus-mosquito vector-host interactions and the impact on transmission and disease pathogenesis of arboviruses. Front Microbiol 10:22 Article Google Scholar Isaac RE, Schoofs L, Williams TA, Corvol P, Veelaert D, Sajid M, Coates D (1998) Toward a role for angiotensin-converting enzyme in insects. Ann N Y Acad Sci 839:288–292 CAS Article Google Scholar Jin YH, Cai L, Cheng ZS, Cheng H, Deng T, Fan YP, Fang C, Huang D, Huang LQ, Huang Q, Han Y, Hu B, Hu F, Li BH, Li YR, Liang K, Lin LK, Luo LS, Ma J, Ma LL, Peng ZY, Pan YB, Pan ZY, Ren XQ, Sun HM, Wang Y, Wang YY, Weng H, Wei CJ, Wu DF, Xia J, Xiong Y, Xu HB, Yao XM, Yuan YF, Ye TS, Zhang XC, Zhang YW, Zhang YG, Zhang HM, Zhao Y, Zhao MJ, Zi H, Zeng XT, Wang YY, Wang XH, For the Zhongnan Hospital of Wuhan University Novel Coronavirus Management and Research Team, Evidence-Based Medicine Chapter of China International Exchange and Promotive Association for Medical and Health Care (CPAM) (2020) A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version). Mil Med Res 7:4 CAS PubMed PubMed Central Google Scholar Kozuch O, Nosek J, Gresikova M, Labuda M, Sekeyova M, Chmela J (1979) Isolation of Tettnang virus from ticks, mosquitoes and small rodents. Acta Virol 23:86–88 CAS PubMed Google Scholar Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD, Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY, Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ, SARS Working Group (2003) A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 348:1953–1966 CAS Article Google Scholar Luby JP, Clinton R, Kurtz S (1999) Adaptation of human enteric coronavirus to growth in cell lines. J Clin Virol 12:43–51 CAS Article Google Scholar Wang J, Wang D, Chen GC, Tao XW, Zeng LK (2020) SARS-CoV-2 infection with gastrointestinal symptoms as the first manifestation in a neonate. Chin J Contemp Pediatr 22:211–214 (in Chinese) Google Scholar Xiao K, Zhai J, Feng Y, Zhou N, Zhang X, Zou JJ, Li N, Guo Y, Li X, Shen X, Zhang Z, Shu F, Huang W, Li Y, Zhang Z, Chen RA, Wu YJ, Peng SM, Huang M, Xie WJ, Cai QH, Hou FH, Chen W, Xiao L, Shen Y (2020) Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins. Nature. https://doi.org/10.1038/s41586-020-2313-x Article PubMed PubMed Central Google Scholar Zhang T, Wu Q, Zhang Z (2020) Probable pangolin origin of SARS-CoV-2 associated with the COVID-19 outbreak. Curr Biol 30(1346–1351):e1342 Google Scholar Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL (2020) A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579:270–273 CAS Article Google Scholar Download references The project is completed by National Biosafety Laboratory, Wuhan, Chinese Academy of Sciences. We are particularly grateful to the running team of the laboratory for their work. We sincerely thank Prof. Zhengli Shi of Wuhan Institute of virology, Chinese Academy of Sciences, for providing us a clinical isolate of SARS-CoV-2 and antibody for this study. This work was supported by the Ministry of Science and Technology of the People’s Republic of China (2020YFC08845600); the National Health Commission of the People’s Republic of China (2018ZX10711001-006). Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China Han Xia, Evans Atoni, Lu Zhao, Nanjie Ren, Doudou Huang, Rongjuan Pei, Zhen Chen, Jin Xiong, Raphael Nyaruaba, Shuqi Xiao, Bo Zhang & Zhiming Yuan University of Chinese Academy of Sciences, Beijing, 100049, China Han Xia, Evans Atoni, Lu Zhao, Nanjie Ren, Rongjuan Pei, Raphael Nyaruaba, Bo Zhang & Zhiming Yuan You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You...

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Han Xia; Evans Atoni; Lu Zhao; Nanjie Ren; Doudou Huang; Rongjuan Pei; Zhen Chen; Jin Xiong; Raphael Nyaruaba; Shuqi Xiao; Bo Zhang; Zhiming Yuan. SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan. Virologica Sinica 2020, 35, 355 -358.

AMA Style

Han Xia, Evans Atoni, Lu Zhao, Nanjie Ren, Doudou Huang, Rongjuan Pei, Zhen Chen, Jin Xiong, Raphael Nyaruaba, Shuqi Xiao, Bo Zhang, Zhiming Yuan. SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan. Virologica Sinica. 2020; 35 (3):355-358.

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Han Xia; Evans Atoni; Lu Zhao; Nanjie Ren; Doudou Huang; Rongjuan Pei; Zhen Chen; Jin Xiong; Raphael Nyaruaba; Shuqi Xiao; Bo Zhang; Zhiming Yuan. 2020. "SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan." Virologica Sinica 35, no. 3: 355-358.

Journal article
Published: 26 May 2020 in Nature
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An outbreak of the coronavirus disease 2019 (COVID-19)1–3, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)4 spread globally. Countermeasures are needed to treat and prevent further dissemination of the virus. In this study, we report the isolation of 2 specific human monoclonal antibodies (MAbs) from a convalescent COVID-19 patient. CA1 and CB6 demonstrated potent SARS-CoV-2-specific neutralization activity in vitro against SARS-CoV-2. In addition, CB6 inhibited SARS-CoV-2 infection in rhesus monkeys at both prophylactic and treatment settings. Further structural studies revealed that CB6 recognizes an epitope that overlaps with angiotensin converting enzyme 2 (ACE2)-binding sites in SARS-CoV-2 receptor binding domain (RBD), thereby interfering with the virus/receptor interactions by both steric hindrance and direct interface-residue competition. Our results suggest CB6 deserves further clinical translation.

ACS Style

Rui Shi; Chao Shan; Xiaomin Duan; Zhihai Chen; Peipei Liu; Jinwen Song; Tao Song; Xiaoshan Bi; Lianao Wu; Ge Gao; Xue Hu; Yanan Zhang; Zhou Tong; Weijin Huang; William Jun Liu; Guizhen Wu; Bo Zhang; Lan Wang; Jianxun Qi; Hui Feng; Fu-Sheng Wang; Qihui Wang; George Fu Gao; Zhiming Yuan; Jinghua Yan. A human neutralizing antibody targets the receptor-binding site of SARS-CoV-2. Nature 2020, 584, 120 -124.

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Rui Shi, Chao Shan, Xiaomin Duan, Zhihai Chen, Peipei Liu, Jinwen Song, Tao Song, Xiaoshan Bi, Lianao Wu, Ge Gao, Xue Hu, Yanan Zhang, Zhou Tong, Weijin Huang, William Jun Liu, Guizhen Wu, Bo Zhang, Lan Wang, Jianxun Qi, Hui Feng, Fu-Sheng Wang, Qihui Wang, George Fu Gao, Zhiming Yuan, Jinghua Yan. A human neutralizing antibody targets the receptor-binding site of SARS-CoV-2. Nature. 2020; 584 (7819):120-124.

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Rui Shi; Chao Shan; Xiaomin Duan; Zhihai Chen; Peipei Liu; Jinwen Song; Tao Song; Xiaoshan Bi; Lianao Wu; Ge Gao; Xue Hu; Yanan Zhang; Zhou Tong; Weijin Huang; William Jun Liu; Guizhen Wu; Bo Zhang; Lan Wang; Jianxun Qi; Hui Feng; Fu-Sheng Wang; Qihui Wang; George Fu Gao; Zhiming Yuan; Jinghua Yan. 2020. "A human neutralizing antibody targets the receptor-binding site of SARS-CoV-2." Nature 584, no. 7819: 120-124.

Journal article
Published: 28 February 2020 in Sustainability
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In this work, the impact of exogenous aerobic bacteria mixture (EABM) on municipal solid waste (MSW) is well evaluated in the following aspects: biogas production, leachate analysis, organic waste degradation, EABM population, and the composition of microbial communities. The study was designed and performed as follows: the control bioreactor (R1) was filled up with MSW and the culture medium of EABM and the experimental bioreactor (R2) was filled up with MSW and EABM. The data suggests that the composition of microbial communities (bacterial and methanogenic) in R1 and R2 were similar at day 0, while the addition of EABM in R2 led to a differential abundance of Bacillus cereus, Bacillus subtilis, Staphylococcus saprophyticus, Staphlyoccus xylosus, and Pantoea agglomerans in two bioreactors. The population of exogenous aerobic bacteria in R2 greatly increased during hydrolysis and acidogenesis stages, and subsequently increased the degradation of volatile solid (VS), protein, lipid, and lignin by 59.25%, 25.68%, 60.47%, and 197.62%, respectively, compared to R1. The duration of hydrolysis and acidogenesis in R2 was 33.33% shorter than that in R1. At the end of the study, the accumulative methane yield in R2 (494.4 L) was almost three times more than that in R1 (187.4 L). In addition, the abundance of acetoclasic methanogens increased at acetogenesis and methanogenesis stages in both bioreactors, which indicates that acetoclasic methanogens (especially Methanoseata) could contribute to methane production. This study demonstrates that EABM can accelerate organic waste degradation to promote MSW biodegradation and methane production. Moreover, the operational parameters helped EABM to generate 20.85% more in accumulative methane yield. With a better understanding of how EABM affects MSW and the composition of bacterial community, this study offers a potential practical approach to MSW disposal and cleaner energy generation worldwide.

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Sai Ge; Jun Ma; Lei Liu; Zhiming Yuan. The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing. Sustainability 2020, 12, 1815 .

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Sai Ge, Jun Ma, Lei Liu, Zhiming Yuan. The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing. Sustainability. 2020; 12 (5):1815.

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Sai Ge; Jun Ma; Lei Liu; Zhiming Yuan. 2020. "The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing." Sustainability 12, no. 5: 1815.

Letter
Published: 01 January 2020 in Emerging Microbes & Infections
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Ya-Nan Zhang; Qiu-Yan Zhang; Xiao-Dan Li; Jin Xiong; Shu-Qi Xiao; Zhen Wang; Zhe-Rui Zhang; Cheng-Lin Deng; Xing-Lou Yang; Hong-Ping Wei; Zhi-Ming Yuan; Han-Qing Ye; Bo Zhang. Gemcitabine, lycorine and oxysophoridine inhibit novel coronavirus (SARS-CoV-2) in cell culture. Emerging Microbes & Infections 2020, 9, 1170 -1173.

AMA Style

Ya-Nan Zhang, Qiu-Yan Zhang, Xiao-Dan Li, Jin Xiong, Shu-Qi Xiao, Zhen Wang, Zhe-Rui Zhang, Cheng-Lin Deng, Xing-Lou Yang, Hong-Ping Wei, Zhi-Ming Yuan, Han-Qing Ye, Bo Zhang. Gemcitabine, lycorine and oxysophoridine inhibit novel coronavirus (SARS-CoV-2) in cell culture. Emerging Microbes & Infections. 2020; 9 (1):1170-1173.

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Ya-Nan Zhang; Qiu-Yan Zhang; Xiao-Dan Li; Jin Xiong; Shu-Qi Xiao; Zhen Wang; Zhe-Rui Zhang; Cheng-Lin Deng; Xing-Lou Yang; Hong-Ping Wei; Zhi-Ming Yuan; Han-Qing Ye; Bo Zhang. 2020. "Gemcitabine, lycorine and oxysophoridine inhibit novel coronavirus (SARS-CoV-2) in cell culture." Emerging Microbes & Infections 9, no. 1: 1170-1173.

Journal article
Published: 07 September 2019 in Insects
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Propoxur-sel strains of Culex pipiens quinquefasciatus were derived from a lab-bred strain following 16 generations of propoxur exposure under sublethal concentrations of LC25 (lethal concentration of 25%) and LC50 (lethal concentration of 50%), respectively. This resulted in resistance development in F16 with ratios of 8.8× and 6.3×, respectively, compared with F0. The fecundity, longevity, sex ratio (F/M), and hatchability of the propoxur-exposed Cx. quinquefasciatus adult survivors and their offspring were decreased, with no effect on the emergence ratio and pupa survival rate. In addition, the intrinsic rates of increase (r), the net reproduction (R0), and the finite rate of increase (λ) of the Cx. quinquefasciatus offspring generations were also decreased significantly compared to F0. Correspondingly, the mean generation time (T) and the population double time (DT) in propoxur-sels were increased. Enhanced activities of cytochrome P450 monooxygenase and esterase were also observed in propoxur-sels, indicating that a detoxification mechanism might be responsible for resistance development in Cx. quinquefasciatus. Except for the three genes cyp4d42v1, cyp4c52v1, and cyp6aa9 which displayed a coincidence in some degree in different treatments, induction by different doses of propoxur and constitutive expression in different generations of propoxur-sel strains resulted in an inconsistent identification of the P450 genes probably related with resistance.

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Xiaolei Zhang; Samuel Karungu; Quanxin Cai; Zhiming Yuan; Xiaomin Hu. Effects of Propoxur Exposure on Insecticidal Susceptibility and Developmental Traits in Culex pipiens quinquefasciatus. Insects 2019, 10, 288 .

AMA Style

Xiaolei Zhang, Samuel Karungu, Quanxin Cai, Zhiming Yuan, Xiaomin Hu. Effects of Propoxur Exposure on Insecticidal Susceptibility and Developmental Traits in Culex pipiens quinquefasciatus. Insects. 2019; 10 (9):288.

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Xiaolei Zhang; Samuel Karungu; Quanxin Cai; Zhiming Yuan; Xiaomin Hu. 2019. "Effects of Propoxur Exposure on Insecticidal Susceptibility and Developmental Traits in Culex pipiens quinquefasciatus." Insects 10, no. 9: 288.