Medicinal and aromatic plants (MAPs) are able to synthesize a diverse group of secondary metabolites (SMs) such as terpenoids or terpenes, steroids, phenolics, and alkaloids with a broad range of therapeutic and pharmacological potentials. Extensive use of MAPs in various industries makes it important to re-evaluate their research, development, production, and use. In intensive agricultural systems, increasing plant productivity is highly dependent on the application of chemical inputs. Extreme use of chemical or synthetic fertilizers, especially higher doses of N fertilization, decrease the yield of bioactive compounds in MAPs. The plant–soil microbial interaction is an eco-friendly strategy to decrease the demand of chemical fertilizers. Arbuscular mycorrhizal fungi (AMF), belongs to phylum Glomeromycota, can form mutualistic symbiotic associations with more than 80% of plant species. The AMF–plant symbiotic association, in addition to increasing nutrient and water uptake, reprograms the metabolic pathways of plants and changes the concentration of primary and secondary metabolites of medicinal and aromatic plants. The major findings reported that inoculation of AMF with MAPs enhanced secondary metabolites directly by increasing nutrient and water uptake and also improving photosynthesis capacity or indirectly by stimulating SMs’ biosynthetic pathways through changes in phytohormonal concentrations and production of signaling molecules. Overall, the AMF-MAPs symbiotic association can be used as new eco-friendly technologies in sustainable agricultural systems for improving the quantity and quality of MAPs.

Drought stress is known as a major yield-limiting factor in crop production that threatens food security worldwide. Arbuscular mycorrhizal fungi (AMF) and titanium dioxide (TiO₂) have shown to alleviate the effects of drought stress on plants, but information regarding their co-addition to minimize the effects of drought stress on plants is scant. Here, a two-year field experiment was conducted in 2019 and 2020 to evaluate the influence of different irrigation regimes and fertilizer sources on the EO quantity and quality of sage (Salvia officinalis L.). The experiment was laid out as a split plot arranged in a randomized complete block design with three replicates. The irrigation treatments were 25, 50, and 75% maximum allowable depletion (MAD) percentage of the soil available water as non-stress (MAD₂₅), moderate (MAD₅₀), and severe (MAD₇₅) water stress, respectively. Subplots were four fertilizer sources including no-fertilizer control, TiO₂ nanoparticles (100 mg L⁻¹), AMF inoculation, and co-addition of TiO₂ and AMF (TiO₂ + AMF). Moderate and severe drought stress decreased sage dry matter yield (DMY) by 30 and 65%, respectively. In contrast, application of TiO₂ + AMF increased DMY and water use efficiency (WUE) by 35 and 35%, respectively, compared to the unfertilized treatment. The highest EO content (1.483%), yield (2.52 g m⁻²), and cis-thujone (35.84%, main EO constituent of sage) was obtained in MAD₅₀ fertilized with TiO₂ + AMF. In addition, the net income index increased by 44, 47, and 76% with application of TiO₂ nanoparticles, AMF, and co-addition of TiO₂ + AMF, respectively. Overall, the integrative application of the biofertilizer and nanoparticles (TiO₂ + AMF) can be recommended as a sustainable strategy for increasing net income and improving EO productivity and quality of sage plants in drought stress conditions. Future policy discussions should focus on incentivizing growers for replacing synthetic fertilizers with proven nano and biofertilizers to reduce environmental footprints and enhance the sustainability of sage production, especially in drought conditions.

Intercropping of medicinal plants/legumes along with bio-fertilizer application is a relatively new sustainable practice for improving the yield and secondary metabolites production. Here, a 2-years field experiment was performed to evaluate the effects of water deficit stress and arbuscular mycorrhizal fungi (AMF) application (as bio-fertilizer) on nutrients concentration, dry matter yield, essential oil quantity and quality of thyme in intercropping with soybean. Three irrigation levels, including (i) irrigation after depletion of 20% (I₂₀) as non-stressed, 50% (I₅₀) as moderate water deficit and 80% (I₈₀) available water as severe water deficit were applied as the main factor. The sub-factor was represented by different cropping patterns including thyme sole culture, replacement intercrop ratio of 50:50 and 66:34 (soybean: thyme) and the third factor was non-usage (control) and usage of AMF. According to our results, the thyme dry yield under moderate and severe water deficit stress decreased by 35 and 44% in the first year, and by 27 and 40% in the second year compared with non-stressed (I₂₀) plants, respectively. Also, the macro- and micro-nutrients of thyme leaves increased significantly in intercropping patterns after application of AMF. The maximum essential oil percentage of thyme was achieved in 50:50 intercropping ratio treated with AMF. Under moderate and severe water deficits, the major constituents of thyme essential oil including thymol, p-cymene and γ-terpinene were increased in intercropping patterns treated with AMF. Generally, AMF application in intercropping ratio of 50:50 may be proposed to farmers as an eco-friendly approach to achieve desirable essential oil quality and quantity in thyme under water deficit stress conditions.

Drought stress (DS) negatively affects plant growth, productivity, and quality in semi-arid and arid regions. Nowadays, application of biofertilizers and stress-modulating nanoparticles (NPs) improves plant performance under stressful conditions. The study evaluated the impacts of arbuscular mycorrhizal fungi (Myco-Root) and TiO₂ NPs on the nutrient uptake, dry yield, essential oil (EO) productivity, and EO quality of peppermint (Mentha piperita L.) under different irrigation regimes. The treatments included three irrigation regimes containing irrigation after 20% (I₂₀, well-watered), 40% (I₄₀, mild DS), and 60% (I₆₀, severe DS) maximum allowable depletion (MAD) percentage of the soil’s available water as well as four fertilizer sources contain no fertilization (control), Myco-Root biofertilizer, TiO₂ NPs, and an integrative application of Myco-Root + TiO₂ NPs. The results demonstrated that the highest (195.72 g m⁻²) and the lowest dry yield (78.76 g m⁻²) of peppermint was obtained in well-watered conditions with integrative application of Myco-Root + TiO₂ NPs and severe drought stress (I₆₀) without fertilization, respectively. The dry yield of peppermint was reduced by 27.7 and 53.4% in mild (I₄₀) and severe drought stress (I₆₀), respectively. The maximum EO content (1.49%) and EO yield (2.30 g m⁻²) was recorded in mild drought stress (I₄₀) treated with Myco-Root + TiO₂ NPs. Based on the GC-MS and GC-FID analysis, 29 constituents were identified in peppermint EO, with the major constituents being menthol (38.99–52%), menthone (12.72–20.13%), 1,8-cineole (6.55–7.84%), and neo-menthol (3.14–4.52%), respectively. The maximum content of menthol, 1,8-cineole, and neo-menthol was obtained under mild drought stress (I₄₀) fertilized with Myco-Root + TiO₂ NPs. The results indicate that the integrative application of Myco-Root + TiO₂ NPs could be used as an alternative method of using chemical fertilizers in sustainable agricultural systems for improving the EO quantity and quality of peppermint grown under drought stress conditions.