Gossypium hirsutum L. is a worldwide economical crop; however, premature leaf senescence reduces its production and quality which is regulated by stresses, hormones, and genes. DNA binding with the one zinc finger (Dof) transcription factors (TFs) participate widely in plant development and responses to biotic and abiotic stresses, but there have been few reports of these TFs in cotton. Here, we perform a genome-wide study of G. Hirsutum L. Dof (GhDof) genes and analyze their phylogeny, duplication, and expression. In total, 114 GhDof genes have been identified and classified into nine subgroups (A, B1, B2.2, B2.1, C1, C2.1, C2.2, D1, and D2) based on phylogenetic analysis. An MCScanX analysis showed that the GhDof genes expanded due to segmental duplications. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that GhDofD9.6 was not only differentially expressed between CCRI10 (with premature senescence) and Liao4086 (without premature senescence) but also responded to salinity stress; GhDofA5.7, GhDofA7.4, GhDofA8.2, GhDof11.1, GhDofD7.2, and GhDofD11.3 signfificantly responded to cold (4 °C) stress. This work lays the foundation for further analysis of the function of GhDof genes in G. hirsutum, which will be helpful for improving the production and quality of cotton.

University campuses feature spatially diverse environments where thermal performance varies seasonally and spatially. In this study, we integrate field measurements with ENVI-met simulations to evaluate how sky view factor (SVF) influences microclimate and outdoor thermal comfort-quantified via air temperature (Ta), mean radiant temperature (Tmrt), wind speed (WS), relative humidity (RH), physiologically equivalent temperature (PET), and the Universal Thermal Climate Index (UTCI)-within urban street and urban building spaces on a temperate Chinese campus. The results reveal contrasting thermal responses: in summer, low-SVF urban street spaces (SVF_avg 0.075) exhibit moderate heat stress (PET_avg 34.5–39.5 °C) due to radiative trapping and limited ventilation, whereas high-SVF urban building spaces (SVF_avg 0.159) face greater heat load and stronger thermal stress, with peak PET exceeding 49.9 °C. In winter, high-SVF urban building spaces benefit from solar gain, improving thermal comfort. Statistical analyses indicate non-linear threshold effects of SVF on comfort indices, with summer comfort positively correlated at SVF > 0.2, and winter comfort negatively associated at SVF ≤ 0.4. These findings identify SVF as a key geometric predictor of seasonal thermal comfort in distinct campus spatial types, provide quantitative thresholds to guide climate-resilient campus planning in warm temperate zone.

Libanotis buchtormensis (Fisch.) DC. is a traditional Chinese medicinal herb used for the treatment of rheumatism, articular pain, and symptoms of the common cold. To evaluate the possible medicinal value of the aerial parts of L. buchtormensis, the chemical composition and antibacterial activity were assessed on a tissue and growth specific basis. Results based on high performance liquid chromatography (HPLC) showed that the samples were divided into two main clusters, one associated with roots and the other associated with the aerial parts. Analysis of the bioactive chemical contents revealed that coumarin was the most abundant compound in leaves (0.53 to 0.82%), while osthole and isoimperatorin exhibited relatively high concentrations in roots. Two-year growth samples, which correspond with the harvest period of L. buchtormensis, contained the highest amounts of total coumarin, osthole, and isoimperatorin. Broth microdilution tests showed that Gram-positive bacterial strains (Staphylococcus aureus, S. epidermidis and Streptococcus agalactiae) were more sensitive to L. buchtormensis extracts than the Gram-negative bacterial strain (Escherichia coli). Staphylococcus aureus and S. epidermidis were more sensitive to the extracts, exhibiting significant correlation with osthole and isoimperatorin contents. Comparative analysis of the chemical profiles and antibacterial activity revealed significant differences between the root and the aerial parts, which implied that the clinical application of the aerial parts needed further study. In addition, the aerial parts could serve as a material source for osthole, having comparable content to that of the root.