The composition of gut microbiota is not a static state in humans but fluctuates in response to changes in environments, diet, and lifestyle factors. Here, we explored differences in gut microbiota between populations worked offshore and onshore and further studied microbiota-associated variables in offshore workers (OFWs). We investigated the gut microbiota of 168 healthy subjects (offshore: 145 and onshore: 23) using 16S rRNA sequencing. Our results indicated that the marine environment caused significant changes in intestinal microbial structure, which was mainly reflected in the increase in bacterial diversity, changes in composition, and the emergence of more specific bacteria in OFWs. In addition, characteristics of gut microbiota in OFWs were further explored, and the genus Holdemanella was considered a potential contributor to the stable state of health. Besides, some dietary factors, namely, duck, mutton, dairy products, and algae vegetables were identified as the gut microbial covariates in the OFWs cohort and were positively correlated with the genus Holdemanella. This suggests the positive intervention of diet on Holdemanella. Our data highlight, for the first time to our knowledge, that the marine geographical environment plays an important role in shaping the gut mycobiome composition. And diet could be considered as the targeted intervention that alters the composition of the microbiome to improve host health.

In order to verify the effect of the new green and low-carbon hydrogel dressing on promoting wound healing, this project applied a sodium alginate hydrogel dressing product containing Escherichia coli and taro toxin analgesic polypeptide (The specific ingredients of the dressing) to skin wounds in common rats. Effects of the hydrogel dressing on promoting skin wound healing was evaluated by observing the occurrence and frequency of behavioral changes in rats, observing wwhistological sections under a high-power microscope, changes in serum cytokine indicators, and Image J analysis of collagen fiber reconstruction ratios in tissue sections. Through comprehensive evaluation, it can be found that hydrogel dressing has analgesic, anti-inflammatory and anti-infection effects on rat wound surface, and acts on promoting wound healing, promoting the formation of new blood vessels in the damaged skin tissue area, promoting the growth of granulation tissue, and promoting the reconstruction of collagen fibers in wound tissue.