This study was conducted in order to study the effect of different concentrations of lead pollution on the photosynthetic characteristics and growth of Miscanthus floridulus, and to reveal its photosynthetic adaptability to lead stress. The differences of gas exchange parameters, chlorophyll fluorescence characteristics and photosynthetic pigment of two Miscanthus floridulus populations, one population from Boluo an uncontaminated site, and another population from Dabaoshan, a mine site, were compared and studied through nutrient solution culture experiments treated with heavy metal lead (Pb) in green house. The results showed that (1) under Pb stress, the net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), intercellular carbon dioxide concentration (Ci), and chlorophyll content (Chl) of the leaves of the two populations decreased in different amplitude. Under moderate and severe Pb stress (80 mg·L⁻¹, 120 mg·L⁻¹, 240 mg·L⁻¹), the plant biomass of non-mining population and mining population plants were 54.5%, 39.7%, 29.4% and 70.4%, 54.7%, 50.9% of the control, respectively. (2) Stomatal restriction was the main factor for the Pn’s decrease in the leaves of the non-mine population under light Pb stress, while the non-stomatal restriction was the main factor for Pn’s decrease in the leaves of the non-mine population under middle and high Pb stresses. (3) Under Pb stress, the maximum photochemical efficiency (Fv/Fm) and potential activity (Fv/Fo) of PS II reaction centers in the two populations of M. floridulus decreased. However, Fv/Fm and Fv/Fo showed a smaller decrease, but the capability to utilize light and the potential to activate PSII of the mine population remained higher than that of the non-mine population. The changes of photochemical quenching coefficient (qP) and non-photochemical quenching coefficient (NPQ) of PSⅡ showed that qP value decreased and NPQ value increased in the two populations under Pb stress. On the whole, the resistance mining area population had a low qP reduction and a large increase in NPQ. Electronic transfer rate (ETR) and PSII actual fluorescence efficiency (ΦPSII) of the mine population changed slightly under Pb stress. These results indicated that when under Pb stress, the electron transport activity and photosynthetic apparatus were damaged less in the mine population with high resistance than in the non-mine population with low resistance. Conclusion: the mining population of M. floridulus has strong tolerance to Pb, which is suitable for the pioneer species of gramineae in vegetation restoration construction in metal mining area.

Through field investigation and experimental analysis, the forms, contents and distribution of heavy metals (Zn, Pb, Cu, Cd, Ni, Cr) in rhizosphere and non-rhizosphere soils of Miscanthus floridulus growing everywhere in Tielongwei mine pond (sample plot 1), Caoduikeng tailings pond (sample plot 2), Donghua tailings pond (sample plot 3) and Small tailings pond (sample plot 4) in Dabaoshan, Guangdong Province were studied. The results showed that the main forms and distributions of heavy metals in rhizosphere and non-rhizosphere soils are basically the same, which shows that the mineral content accounts for most of the total amount of heavy metals, while the exchange content is low. Compared with non-rhizosphere soil, the proportion of exchangeable and organic heavy metals in rhizosphere soil increased significantly, in which the proportion of organic-bound Cu increased by 53.25%, the proportion of organic-bound Cd and Pb increased by more than 17%, and the proportion of Zn increased by 5.67%. At the same time, the contents of carbonate-bound and iron manganese oxide-bound decreased. Statistical analyses showed that the morphological distribution of Zn, Pb, Cu, Cd, Ni and Cr in rhizosphere soil was closely related to soil pH value, organic matter content, plant growth and other factors. The results of this study provided a basis for the restoration of heavy metal-contaminated sites by Miscanthus.

The activation of peracetic acid (PAA) to generate highly reactive species has emerged as a promising advanced oxidation process (AOP) for the degradation of refractory organic pollutants. This review systematically summarizes the recent advancements in PAA-based AOPs, encompassing various activation strategies, underlying reaction mechanisms, and applications across different environmental matrices. The activation methods are critically discussed, including direct energy activation, homogeneous catalysis, and heterogeneous catalysis. The generation process of diverse reactive species, like hydroxyl radicals (HO·), organic radicals (CH₃C(O)O·, CH₃C(O)OO·), and singlet oxygen (¹O₂), was introduced, and their oxidation selectivity and anti-interference ability were compared. Furthermore, the practical applications of PAA-based AOPs in treating wastewater, groundwater, and contaminated soil/sediments are reviewed. Finally, this review outlines critical challenges, including potential toxic byproduct formation, catalyst stability, and economic feasibility, and proposes future research directions to facilitate the transition of PAA-based AOPs from laboratory-scale research to full-scale implementation. This review provides insights for developing efficient, selective, and sustainable oxidation technologies, thereby contributing to the mitigation of emerging contaminant threats and the advancement of environmental remediation practices.

The disordered mining of Dabaoshan lead-zinc mineral resources in Shaoguan has brought serious harm to the regional ecological environment. In order to investigate the heavy metal pollution status and microbial characteristics of soil plant system in mining area, The distribution of heavy metals in the soil, the activity of soil microorganisms and the accumulation characteristics of heavy metals in the dominant plant Miscanthus floridulus were studied. The results indicated that metal element contents of Miscanthus floridulus in sequence were: Zn>Pb>Cu> Cd. This study demonstrated that the elemental content of the Miscanthus floridulus plant showed Zn>Pb>Cu>Cd, with Zn being the most significantly correlated with soil elements, followed by Pb. Compared with the control group, the Miscanthus floridulus-soil system possessed obviously different soil microbial features: intensiver in microbial basal respiration strength, and higher microbial eco-physiological parameters Cmic/Corg and qCO₂, but lower in soil microbial biomass. The results showed the soil enzymatic activities decreased significantly with increase of contamination of heavy metals, especially dehydrogenase and urease activities. With the increase of the content of heavy metals in the mining area soil, the intensity of soil biochemical action in the mining area (Q1, Q2) soil decreased significantly, and the biochemical action showed a significant negative correlation with the content of heavy metals in the soil. Compared with the non mining area (Q8) soil, the intensity of soil ammonification, nitrification, N fixation and cellulose decomposition decreased by 43.2%~71.1%, 70.1%~92.1%, 58.7%~87.8% and 55.3%~79.8% respectively. The decrease of soil microbial activity weakened the circulation rate and energy flow of C and N nutrients in the soil of the mining area.

Miscanthus floridulus is a plant with a high biomass and heavy metal tolerance, which is a good candidate for phytoremediation. Pot experiments were conducted to compare the growth response, Pb enrichment ability, and the effect on Pb speciation of two ecotypes of M. floridulus from the Dabaoshan Mining Area and the non-mining area of Boluo County, Huizhou, in soils with different Pb contents. The results showed that two ecotypes of M. floridulus had different growth responses to Pb concentrations in soil. Under a low concentration of Pb (100 mg·kg⁻¹) treatment, the aboveground biomass of the non-mining area plant ecotype was significantly affected, while the plants with the mining area ecotype were not significantly affected. When the concentration of Pb increased, the aboveground biomass of the non-mining ecotype was 30.2–41.1% of the control, while that of the mining ecotype was 57.8–65.0% of the control. The root biomass of the non-mining ecotype decreased with the increase of treatment concentration, accounting for 57.8–64.2% of the control, while that of the mining ecotype increased significantly, accounting for 119.5–138.6% of the control. The Pb content in the shoots and roots of the mining ecotype M. floridulus increased rapidly with the increase of the Pb treatment concentration in the soil, and the increase in speed was obviously faster than that of the non-mining ecotype. The total amount of Pb accumulated in the roots of the ecotype from the mining area was much greater than that of the ecotype from the non-mining area, and increased significantly with the increase of Pb concentration in the soil (p < 0.05). With the aggravation of Pb stress, the transfer coefficient and tolerance index of the two ecotypes decreased by different degrees. The transfer coefficient and tolerance index of the mining ecotype were significantly higher than those of the non-mining ecotype. Pearson correlation analysis showed that root biomass was positively correlated with shoot biomass, and shoot biomass was negatively correlated with Pb content in both root and shoot, indicating that Pb accumulation in root and shoot was toxic to plants and inhibited the growth of M. floridulus. The mining ecotypes showed stronger tolerance to and enrichment of Pb.

To reveal the similarities and differences in the resistance mechanisms of different ecotypes to Cu stress, a pot experiment was used to systematically compare the physiological responses of non-mining ecotype Miscanthus floridulus (collected from Boluo County, Huizhou City) and mining ecotype Miscanthus floridulus (collected from Dabaoshan mining area) under different Cu concentrations. The results showed that chlorophyll a, chlorophyll b and total chlorophyll in the leaves of the two ecotypes of M. floridulus were negatively correlated with Cu stress concentration (p < 0.01), but the extent of decrease for the ecotypes in the mining area was lower than that for the ecotypes in the non-mining area. The values of chlorophyll a/b for both ecotypes increased with increasing Cu treatment concentration, indicating that Cu is more harmful to chlorophyll b than to chlorophyll a for M. floridulus. Cu stress can lead to the accumulation of malondialdehyde (MDA) in the leaves of M. floridulus with the amount of MDA accumulation observed being greater in the non-mining ecotype than in the mining ecotype (p < 0.05). The content of antioxidant substances (ascorbic acid and reduced glutathione) in the mining ecotype M. floridulus was significantly higher than that in the non-mining ecotype. The activity of SOD in the leaves of non-mining ecotypes was inhibited by Cu stress and the activity of POD was increased by Cu stress. However, the increase in POD in the mining ecotypes was greater than that in the non-mining ecotypes and the activities of the two enzymes in the mining ecotypes were significantly higher than those in the non-mining ecotypes at the highest concentration of Cu. Cu had different effects on PPO activity in the leaves of the two ecotypes of M. floridulus. The plant leaves of the non-mining ecotype at 400 and 800 mg·kg⁻¹ were significantly fewer than those of the control group (p < 0.05), which were 87.1% and 65.2% of the control group, respectively. The PPO activity in the plant leaves of the mining ecotype was higher than that in the leaves of the non-mining ecotype and was significantly higher at 400 and 800 mg·kg⁻¹ than that of the control group (p < 0.05), at 226.5% and 268.1% of the control group, respectively. These results indicate that the mining ecotype M. floridulus is more resistant to copper stress, that resistant ecotypes have been formed, and that small-molecule antioxidant substances play an important role in increasing resistance levels.