Alessandra Melo de Aguiar graduated in Biological Sciences Medical Modality and earned a master's degree in Morphological Sciences from the Federal University of Rio de Janeiro, where she developed knowledge in cell biology, cell differentiation, and stem cells in disease models. She was responsible for the Cell Culture Sector (INCQS-FIOCRUZ) for two years. She has been developing research on human stem cells and finished a doctorate in Cellular and Molecular Biology at the Federal University of Paraná. She works in innovation and technological development, focusing on cytotoxicity assays and new alternative methods for the use of animals with stem cells. She is a professor in the graduate program in Biosciences and Biotechnology at the Carlos Chagas Institute, FIOCRUZ-PR, in the Cellular and Molecular Biology Program at the Federal University of Paraná, and also at the Federal Technological University of Paraná.