To study the effect of wood creep on the long-term performance of prestressed glulam continuous beams, a 180-day test was carried out on beams configured with different numbers of steel wires (2, 4, 6) and with different prestress values (0, 7, 14 kN). By investigating the stress loss of the steel wires in the beam and the change in the mid-span deflection over time, the factors influencing the creep of the continuous beam were analyzed. Three models were selected to fit the creep process of the test beams. Moreover, the creep deformation coefficient θ was introduced to reflect the influence of glulam creep on the deflection change in the test beams and to predict the total deflection of the beam within 50 years. The results showed that with increasing the number of steel wires and the prestress value on the beams, the total stress of the steel wires declined more and faster. Increasing the number of steel wires or decreasing the prestress force value could effectively restrain the change speed of the mid-span long-term deflection of the beam. Three models were compared, and the power-law equation was the most accurate. At familiar steel wire quantities and force levels, the θ value of the test beams within the design service life of 50 years was determined to be 1.28–2.29.