Microbial enzymes are increasingly finding applications as therapeutics due to their targeted activity and minimal side effects. Serratiopeptidase, also known as a miracle enzyme, has already proved its potential as an anti-inflammatory, mucolytic, fibrinolytic, analgesic in many studies. A cost effective, bioreactor level production process has been described here comprising of the fed-batch fermentation to produce recombinant serratiopeptidase protein expressed as a fusion construct. High yield of cell mass as well as protein was obtained by the optimization of bioreactor parameters. The downstream solubilization and purification processes were also optimized to achieve maximum yield of pure, active serratiopeptidase protein. A final yield of 2.5 ± 0.764 g L⁻¹ of protein was obtained, having 8382 ± 291 U mg⁻¹ of specific caseinolytic activity. Additionally, a novel, unexpected self-proteolytic activity of the enzyme that cleaves the N-terminal 6× His-SUMO fusion tag along with the enzyme propeptide, thus yielding a mature serratiopeptidase, was also found.

Removal of synthetic textile dye poses a challenge to the textile industry and a threat to environment flora and fauna. These dyes are marginally degradable and recalcitrant, hence alternatives to physical and chemical techniques like various bioremediation studies involving plants, plant roots, single or consortium of microbes have been used as environment friendly methods for the removal of textile dye. In the present study potent bacteria for dye decolourization were isolated from rhizospheric soil collected from Kamothe, Navi Mumbai, India. Of the 20 isolates obtained after enrichment, seven isolates were used for further screening of efficient decolourization ability in MBM media containing 10% glucose, 2.5 % trace metal solution and 0.1% of MO dye concentration. Physiological parameters to optimize the decolourization of dye at optimum pH, temperature and incubation time was studied for all the seven isolates. UV-vis and Fourier Transform Infrared spectroscopy were used to investigate dye decolorization. The seven isolates were characterized morphologically, biochemically, and molecular identification of these strains were performed by 16S rRNA sequence analysis. The isolates were identified as Bacillus paramycoides, Pseudomonas taiwanensis, Citrobacter murliniae, Acinetobacter pitti, Exiguobacterium acetylicum, Psychrobacter celer , and Aeromonas taiwanensis . Out of these Aeromonas taiwanensis has shown exceptional capacity by 100% decolorization of azo dye in minimum time. 1. Graphical Highlights of the study: Collection of soil samples from rhizospheric and non-rhizospheric region of the mangroves plant. Enrichment of soil sample in media containing azo dye for dye decolourization. Screening and isolation of potential dye decolourizers. Optimize the condition for dye decolourization. FTIR analysis and application based studies. Identification of microbes using 16SrRNA.

Removal of synthetic textile dye poses a challenge to the textile industry and a threat to environment flora and fauna. These dyes are marginally degradable and recalcitrant, hence alternatives to physical and chemical techniques like various bioremediation studies involving plants, plant roots, single or consortium of microbes have been used as environment friendly methods for the removal of textile dye. In the present study potent bacteria for dye decolourization were isolated from rhizospheric soil collected from Kamothe, Navi Mumbai, India. Of the 20 isolates obtained after enrichment, seven isolates were used for further screening of efficient decolourization ability in MBM media containing 10% glucose, 2.5% trace metal solution and 0.1% of MO dye concentration. Physiological parameters to optimize the decolourization of dye at optimum pH, temperature and incubation time was studied for all the seven isolates. UV-vis and Fourier Transform Infrared spectroscopy were used to investigate dye decolorization. The seven isolates were characterized morphologically, biochemically, and molecular identification of these strains were performed by 16S rRNA sequence analysis. The isolates were identified as Bacillus paramycoides, Pseudomonas taiwanensis, Citrobacter murliniae, Acinetobacter pitti, Exiguobacterium acetylicum, Psychrobacter celer, and Aeromonas taiwanensis. Out of these Aeromonas taiwanensis has shown exceptional capacity by 100% decolorization of azo dye in minimum time.